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Compositions and Methods to Promote Implantation and Engrafment of Stem Cells

a technology of stem cells and compositions, applied in the field of compositions and methods to promote tissue repair, can solve the problems of more damage than actual repair of acl, inability to achieve clinical care, and limited practical success of procedures in health care settings, so as to facilitate tissue repair and/or replacement

Inactive Publication Date: 2011-08-18
REGENERATIVE SCI LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The present invention provides repair compositions for tissue repair and replacement in patients in need. The compositions include a cell growth enhancing composition and an inflammation inducing agent. The cell growth enhancing composition can include autologous or non-autologous materials, such as platelet rich fibrin solutions or recombinant growth factors. The inflammation inducing agents can include osmolar agents, inflammatory cytokines, or sclerosing agents. The repair compositions can also include essential nutrients and stem cells for further tissue growth signaling and repair. The methods involve harvesting and preparing the repair composition, administering it to the repair site, and optionally administering stem cells or other repair-like cells to enhance the repair process. The pharmaceutical compositions can be used to treat patients with injuries or degenerative diseases."

Problems solved by technology

However, these studies utilized a surgical approach to include a gross tissue injury which was shown to signal MSCs to the injury site, this approach is, however, likely impractical for clinical care (for example cutting portions of an ACL ligament to signal MSC homing to the ACL site could result in more damage than actual repair to the ACL).
However, these procedures have had limited practical success in the health care setting.
Unfortunately, mere implantation of stem cells to a site in this manner has proven mostly ineffective.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Therapeutic use of Embodiments of the Present Invention

[0055]Approximately 20 ml of whole bone marrow was extracted from two patients, CD (40 year old, white male) and JV (28 year old, white male). CD held a pre-op diagnosis of severe osteoarthritis of the knee with evidence of myxoid degeneration of the medial>lateral meniscus and JV held the pre-op diagnosis of a chronic bucket handle tear of the posterior horn of the medial meniscus.

[0056]Each patient was then placed prone on an OR table and the area to be harvested was numbed with 1% Lidocaine, and a sterile disposable trocar was used to draw 10 cc of marrow blood from the right PSIS area and 10 cc from the left PSIS area.

[0057]Whole marrow was centrifuged at 100 g for 4-6 minutes to separate the plasma from the RBCs. The plasma was removed, placed in a separate tube, and centrifuged at 1000 g for 10 minutes to pellet the nucleated cell fraction. The nucleated cells were washed once in PBS, counted, and then resuspended in DMEM+...

example 2

MSCs Expand in Presence of Dextrose

[0064]To ensure that the various growth factors commonly found in platelet lysate (TGF-beta, FGF, IGF, PDGF) could be exposed to a hypertonic environment and still function to support mesenchymal stem cell growth, the following experiment was carried out with culture expanded human MSCs:

Method:

[0065]To 0.8 mL of 10% platelet lysate was added 0.2 mL of 50% Dextrose. In a separate condition, to 0.8 ml of 10% platelet lysate (PL) we added 0.2 mL of Phosphate Buffered Saline. We allowed the two samples to incubate 1 hr at 37 C in a 5% CO2 environment. 1 mL of each suspension was then removed and added to 9 mis of basic alpha mem media to get final ratio of 10% PL and 1% Dextrose. Each well of a 6 well plate was then seeded with 100,000 cells in each suspension. After 48 hr incubation all of the cells appeared morphologically normal.

Results:

[0066]

StdPL+PL−AverageDevCV2.50E+051.90E+052.20E+053000013.636PL+ = with DextrosePL− = control

The example shows th...

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PUM

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Abstract

Tissue repair in-vivo depends on acute inflammation, but in many clinical situations the other major components of healing such as blood supply, anabolic hormones, growth factors, and stem cells are lacking. This invention includes compositions consisting of an agent which induces an inflammatory healing response combined with an autologous platelet lysate at a specific concentration which may have demonstrated in-vitro abilities to expand autologous tissue repair cells.

Description

TECHNICAL FIELD[0001]The invention generally relates to compositions and methods for induction of tissue repair in a patient in need thereof. More particularly, the invention relates to compositions and methods for inducing diffuse micro-tissue injury and enhanced cell growth at a site to facilitate in-vivo tissue repair and / or replacement in a patient in need thereof.BACKGROUND OF THE INVENTION[0002]Mesenchymal stem cells (MSCs) are pluripotent blast or embryonic-like cells located in blood, bone marrow, dermis and periosteum. In general these cells are capable of renewing themselves over extended periods of time as well as, under various environmental conditions, differentiating into cartilage, bone and other connective tissue. Recently, various investigators have researched the potential for using these cells to repair or regenerate target tissues, e.g., bone, cartilage, cardiac muscle, etc. In this manner MSCs have been reported to have regenerative capabilities in a number of a...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/14A61K9/00A61K38/18A61P17/02A61P29/00A61P43/00A61K35/16A61K35/19
CPCA61K35/16A61K35/19A61K38/19A61K38/363A61K45/06A61K31/7004A61K31/05A61K2300/00A61P17/02A61P29/00A61P43/00
Inventor CENTENO, CHRISTOPHER J.
Owner REGENERATIVE SCI LLC
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