The invention discloses a 
litopenaeus vannamei ecological breeding method based on 
live food and 
water quality bio-regulation. The method includes following steps: (1), treating water before breeding,and performing 
live food culture; (2), arranging larvae; (3), breeding, regulating 
water quality, and preventing and controlling pest and 
disease; (4), marking thickness, desalting, and transferring.An ecological breeding technique of 
chlamydomonas plus artemia plus probiotics plus compound Chinese herbal 
medicine is provided, and natural 
protein, trace elements and minerals are provided for theshrimp larvae, so that the 
shrimp larvae have strong physique and resistivity, and ecological 
shrimp larvae bred by the method are big in size, orderly in specification, quick in growth and high in 
survival rate. The breeding method is advanced in concept, scientific, reasonable, easy in implementation and suitable for large-scale application in high-health breeding of 
litopenaeus vannamei and has good social benefit and economic benefit.