A kind of co-cultivation method of freshwater rotifers and chlorella

A technology of co-cultivation and chlorella, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve the problem of rare rotifer isolation and control research, inapplicable rotifer safety control research, and great impact major issues

Active Publication Date: 2011-12-14
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And for O 3 The isolation and control of rotifers in the /BAC advanced treatment process is relatively rare
In addition, traditional culture methods are mostly used in actual aquaculture fisheries, which cann

Method used

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  • A kind of co-cultivation method of freshwater rotifers and chlorella
  • A kind of co-cultivation method of freshwater rotifers and chlorella
  • A kind of co-cultivation method of freshwater rotifers and chlorella

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Embodiment 1

[0026] O in this example 3 The dominant rotifer in the / BAC advanced treatment process - the process of indoor perennial cultivation of rotifers. A method for co-cultivating freshwater rotifers and chlorella, the steps of which are:

[0027] 1. O 3 Determination of dominant rotifers in / BAC advanced treatment process:

[0028] From March 2010 to April 2011, Shenzhen used O for a water plant in the south 3 / BAC advanced water treatment process The zooplankton population structure of each process flow in the water plant is investigated for a period of one year, and samples are taken in the middle of each month for quantitative and qualitative analysis. According to the process flow of the water plant, the sampling points are set up with factory water, activated carbon tank filtered water, activated carbon tank, sand filter tank, sedimentation tank, flocculation tank and raw water. Surface water samples and water samples at a depth of 2m are collected respectively.

[0029] Q...

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Abstract

The invention discloses a method for separating dominant rotifers and co-culturing the dominant rotifer and chlorella vulgaris in an O3/BAC (biological activated carbon) advanced treatment process, which comprises the following steps of: a. determining the rotifers in the O3/BAC advanced treatment process: researching population structures of planktonic animals in various process flows of waterplant ozone activated carbon advanced wastewater treatment, sampling and respectively quantitatively and qualitatively analyzing; b. separating the dominant rotifers in the O3/BAC advanced treatment process: acclimating in dark at room temperature over night, picking up health and lively adults by using a dropper for further culturing; c. culturing the chlorella vulgaris: grafting purified single chlorella vulgaris in a Blue-Green Medium culture solution; d. carrying out adaptability on the rotifers: directly grafting the rotifers with the initial grafting density of 10-30/L in the cultured chlorella vulgaris solution; e. co-culturing: co-culturing according to the initial grafting density of the rotifers; and f. subcultring: if the rotifers in a culturing system is dead, transiting the rotifers for subcultring. Co-culturing the dominant rotifers indoors for a longer time in the O3/BAC advanced treatment process is realized, and the density of the rotifers is maintained at 400-600 ind/ml. Particularly, experimental material is provided for safety control and risk assessment research of the rotifers in life drinking water.

Description

technical field [0001] The invention relates to the technical field of zooplankton cultivation, more specifically to a water plant O 3 The co-cultivation method of dominant rotifers and chlorella in BAC advanced treatment process is applicable to the co-cultivation of freshwater rotifers and chlorella. Background technique [0002] Rotifers generally do not cause pollution to water bodies, and they are an integral part of the organic environment of water bodies. Rotifers are the group with the highest reproduction rate and the strongest dispersal ability among multicellular animals, so they tend to become the dominant group of plankton in water bodies. Some rotifers are used as model organisms in the study of population ecology, molecular biology and ecotoxicology indoors, and some freshwater rotifers play an important role as good indicator organisms of water bodies and drinking water quality. [0003] Because rotifers reproduce in large numbers in the water body and roti...

Claims

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Application Information

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IPC IPC(8): A01K67/033C12N1/12C12R1/89
Inventor 黎双飞周连宁胡章立
Owner SHENZHEN UNIV
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