Method for extracting anti-gastric cancer active component from water chestnut shells

A technology of active ingredient and rhombohedral shell is applied in the field of extracting anti-gastric cancer active ingredients in rhombohedral shell, which can solve the problems of no report, unreported application, discount of activity situation, etc., and achieves easy industrial production, good extraction effect, and easy control. Effect

Inactive Publication Date: 2010-10-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the reports on the extraction of anti-gastric cancer active ingredients from water chestnut shells use conventional plant compounds such as flavonoids and polysaccharides as index substances. Anti-gastric cancer activity of shell flavonoids
There has been a long history of studying the anti-gastric cancer effect of a single active ingredient, but there has been no major breakthrough in the anti-gastric cancer activity, and traditional Chinese medicine pays attention to compatibility, and usually the mixture has the strongest activity
Although in vitro experiments have proved that the above-mentioned single active substances have in vitro tumor suppressor activity, the activity is greatly reduced after being digested by the digestive tract, generally only about 30% of the original activity
[0004] At present, there is no report on the application of the semi-bionic extraction method of water chestnut plants, and there is no report on the use of an in vitro gastric cancer cell model as a screen for anti-gastric cancer activity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, a kind of method for extracting anti-gastric cancer active ingredient from water chestnut shell, carries out following steps successively:

[0025] 1) Using 1g of rhomboid shell powder as raw material, add 50ml of glycine-hydrochloric acid buffer solution with pH=2 and 0.01g of pepsin (activity: 3000-3500NF U / mg) to the raw material, and extract 0.5 h, and then all were transferred to a 50 mL centrifuge tube, centrifuged at 3000 rpm for 10 min, and the supernatant was removed to obtain a filter residue.

[0026] 2) Add 50 mL of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with pH=8 and 0.07 g of trypsin (trypsin activity ≥ 250NF U / mg) to the filter residue obtained in step 1), and place in a water bath at 37°C Static extraction for 70 minutes, then all transferred to a 50mL centrifuge tube, centrifuged at 3000 rpm for 10 minutes, and the supernatant was recovered, which was the primary extract with anti-gastric cancer activity. ...

Embodiment 2

[0030] Embodiment 2, a kind of method for extracting anti-gastric cancer active ingredient from water chestnut shell, carries out following steps successively:

[0031] 1) Using 3g of rhomboid shell powder as a raw material, add 30ml of glycine-hydrochloric acid buffer solution with pH=2 and 0.06g of pepsin (activity: 3000-3500NF U / mg) to the raw material, and extract in a water bath at 37°C for 1h , and then all were transferred to a 50mL centrifuge tube, centrifuged at 3000 rpm for 10min, and the supernatant was removed to obtain a filter residue.

[0032] 2) Add 30 mL of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with pH = 8 and 0.12 g of trypsin (trypsin activity ≥ 250NF U / mg) to the filter residue obtained in step 1), and place in a water bath at 37°C Static extraction for 70 min, then all transferred to a 50 mL centrifuge tube, centrifuged at 3000 rpm for 10 min, and the supernatant was recovered, which was the initial extract with anti-gastr...

Embodiment 3

[0036]Embodiment 3, a kind of method for extracting anti-gastric cancer active ingredient from water chestnut shell, carries out following steps successively:

[0037] 1), using 1g of rhomboid shell powder as raw material, add 40ml of glycine-hydrochloric acid buffer solution with pH=2 and 0.1g of pepsin (activity: 3000-3500NF U / mg) to the raw material, extract 1.5 h, and then all were transferred to a 50 mL centrifuge tube, centrifuged at 3000 rpm for 10 min, and the supernatant was removed to obtain a filter residue.

[0038] 2) Add 40 mL of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with pH=8 and 0.05 g of trypsin (trypsin activity ≥ 250NF U / mg) to the filter residue obtained in step 1), and place in a water bath at 37°C Extract statically for 1 hour, then transfer all to a 50 mL centrifuge tube, centrifuge at 3000 rpm for 10 minutes, and recover the supernatant, which is the primary extract with anti-gastric cancer activity.

[0039] 3) Put th...

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PUM

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Abstract

The invention discloses a method for extracting an anti-gastric cancer active component from water chestnut shells. The method sequentially includes the following steps that: (1) water chestnut shell powder is added with buffer solution with a pH value being equal to 2 and pepsin, kept still for extraction under 37DEG C of water bath and centrifugated; (2) filter residue obtained in step 1 is added with buffer solution with a pH value being equal to 8 and trypsin, kept still for extraction under 37DEG C of water bath and centrifugated; and (3) supernate obtained in step 2 is centrifugated at 12000 to 14000 rotations per minute for 25 to 35min, and after centrifugation, the obtained liquid is lyophilized, so that the anti-gastric cancer active component is obtained. The anti-gastric cancer active component extracted by adopting the method is characterized by high activity.

Description

technical field [0001] The invention relates to a method for extracting anti-gastric cancer active components in water chestnut shells. Background technique [0002] Water chestnut is native to the warm regions of Europe and Asia. It is an annual trailing aquatic herb and has been cultivated in my country for more than 3,000 years. At present, the planting area of ​​water chestnut in my country is about 40,000 hectares, and the annual output is about 240,000 tons. The shelling rate of water chestnut is about 30%. According to statistics, the fresh water chestnut shells produced every year can reach 71,000-72,000 tons. A large amount of water chestnut shells have not been effectively utilized, which not only pollutes the environment, but also causes great waste. In recent years, Chinese scholars have discovered through research that water chestnut shells contain functional components such as alkaloids, flavonoids, polysaccharides, and volatile oils. Studies have shown that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185C12Q1/02A61P35/00A61P1/00A61K131/00
Inventor 沈生荣宁颖于海宁
Owner ZHEJIANG UNIV
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