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47 results about "Trypsin activity" patented technology

In 1876, trypsin was first named by Kuhne who described the proteolytic activity of this pancreatic enzyme. He compared trypsin and pepsin, discovering the differentiating factor to be the optimal pH. In 1931, Northrop and Kunitz purified trypsin by crystallization shortly after first purifying pepsin in 1930.

Egg white powder with high foamability and preparation method thereof

The invention provides egg white powder with high foamability and a preparation method thereof and relates to reconstruction of whey protein structure and functional properties, belonging to the technical field of biological processing of foodstuffs. According to the invention, on the basis of preliminary work, enzymatic hydrolysis of lipase in advance and cooperative enzymatic hydrolysis of composite protease are utilized for treatment of egg white; total usage amount of lipase and composite protease is less than usage amount of individually used lipase or protease, and however, foamability and foam stability of egg white powder obtained by combined utilization of lipase and composite protease are higher than those of egg white powder obtained by individual utilization of lipase or composite protease; the egg white powder obtained in the invention can meet demands for high-grade products on the market, and the advantages of a simple process and high cost performance are achieved in the invention. According to the invention, the ratio of active usage amount of Aspergillus oryzae protease, papain and trypsin is determined to be 1:1:1; the usage amount and other technological parameters cooperatively allow egg white powder with high foamability to be obtained; egg white powder with high foamability provided in the invention enables the additional output value of eggs to be increased, lays a technical foundation for development and industrial production of special-purpose egg white powder products and increases economic benefits for enterprises.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Device for simulation of digestion in small intestine and use method thereof

The invention discloses a device for simulation of digestion in small intestine and a use method thereof. The device comprises a small intestine reactor, a stirrer, a water-bath control device, a computer monitoring system and a small intestine evacuation pump. A small intestinal fluid is added into the small intestine reactor, a solution or a dispersion liquid to be detected is added into the small intestine reactor, a simulator simulates small intestine peristalsis, the water-bath control device controls a temperature in the small intestine reactor, the computer monitoring system monitors an internal temperature, a pH value and digestion time of the small intestine reactor, the small intestine evacuation pump simulates small intestine evacuation, the digested product is collected, pancreatin and trypsin activity is eliminated, the digestion simulation process is repeated three times, and a digestion case of the material to be detected is detected. The device realizes first simulation of specific and detailed digestion in small intestine, realizes observation of digestion of various active materials or nutrients in the human small intestine, carries out simulation in a simulation environment designed according to a human physiological environment, has high conformity to the small intestine digestion and has high accuracy in simulation environment control.
Owner:NANCHANG UNIV

Pharmaceutical Compositions and Methods for Reducing Body Fat

InactiveUS20070298025A1Lowering whole body fat contentReduce contentBiocidePeptide/protein ingredientsSerine Protease InhibitorsEnteropeptidase
The invention concerns a method of reducing body fat content of a subject in need thereof, the method comprising administering to the subject an agent capable of down-regulating activity and / or expression of at least one component participating in protein digestion and / or absorption. Such agents may be (i) an oligonucleotide directed to an endogenous nucleic acid sequence expressing said at least one component participating in said protein digestion and / or absorption or (ii) a protease inhibitor directed to said at least one component participating in protein digestion and / or absorption. The invention is particularly directed to a method of reducing body fat content of a subject in need thereof, the method comprising administering to the subject serine protease inhibitor inhibiting both enteropeptidase and trypsin activity.
Owner:OBE THERAPY BIOTECH

Method for detecting trypsinase activity

The invention belongs to the field of biological, and particularly relates to a method for detecting trypsinase activity. A simple and convenient method for detecting the trypsinase activity is builtby using the mutual action differences of polypeptides before and after the trypsinase enzyme digestion and graphene oxide loaded with electrochemical active molecule thionine. The graphene oxide haslarger specific surface area, and can load a great amount of thionine; the effects of amplifying electrochemical signals and improving the detection sensitivity are achieved. The gold electrode surface is modified by gold sulfhydryl bond covalent formed by gold and sulfhydryl of tail end cysteine via substrate polypeptides; the sulfhydryl-containing polypeptides are subjected to covalent modification onto the gold surface; then, 6-mercapto-l-hexanol is used for space occupation; non-specific adsorption polypeptides are removed; the electrode surface blank position is sealed; the N tail end ofthe substrate polypeptide sequence is provided with histidine; the histidine has positive charges; graphene oxide containing rich carboxyls have negative charges after dissociation; stronger static electricity mutual action exists between the histidine and the graphene oxide, so that a prepared compound is adsorbed to the final modified electrode product.
Owner:CHANGZHOU AMANTE CHEM CO LTD

Supermolecular assembly for trypsin based on label-free fluorescence detection and preparation method of supermolecular assembly

The invention relates to a supermolecular assembly for trypsin based on label-free fluorescence detection and a preparation method of the supermolecular assembly and belongs to the technical field of interdisciplinary science of materials, biology and analytical chemistry. The supermolecular assembly is characterized by being compound aqueous solution formed by a quaternary ammonium cationic gemini surfactant and heparin sodium under the electrostatic action. The preparation method comprises the following steps: firstly determining critical micelle concentration of the gemini surfactant, forming the supermolecular assembly by the gemini surfactant and the heparin sodium at the concentration, and enabling a hydrophobic dye Nile red to enter the inner cavity of the assembly, so that fluorescence emission is enhanced; then adding protamine which has specific interaction with the heparin sodium, and disassembling, so that the fluorescence emission intensity is reduced with increase of the concentration of the protamine; and then adding trypsin to hydrolyze the protamine and restore the assembly, so that the fluorescence emission intensity is increased with increase of trypsin concentration, and trypsin activity detection is realized. The supermolecular assembly provided by the invention is simple to prepare and low in cost and has good salt resistance and a broad application prospect.
Owner:TAIYUAN UNIV OF TECH

Egg white powder with high foamability and preparation method thereof

The invention provides egg white powder with high foamability and a preparation method thereof and relates to reconstruction of whey protein structure and functional properties, belonging to the technical field of biological processing of foodstuffs. According to the invention, on the basis of preliminary work, enzymatic hydrolysis of lipase in advance and cooperative enzymatic hydrolysis of composite protease are utilized for treatment of egg white; total usage amount of lipase and composite protease is less than usage amount of individually used lipase or protease, and however, foamability and foam stability of egg white powder obtained by combined utilization of lipase and composite protease are higher than those of egg white powder obtained by individual utilization of lipase or composite protease; the egg white powder obtained in the invention can meet demands for high-grade products on the market, and the advantages of a simple process and high cost performance are achieved in the invention. According to the invention, the ratio of active usage amount of Aspergillus oryzae protease, papain and trypsin is determined to be 1:1:1; the usage amount and other technological parameters cooperatively allow egg white powder with high foamability to be obtained; egg white powder with high foamability provided in the invention enables the additional output value of eggs to be increased, lays a technical foundation for development and industrial production of special-purpose egg white powder products and increases economic benefits for enterprises.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
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