10 results about "Trypsin activity" patented technology

A composition for suppressing trypsin activity is disclosed. The composition contains a bacterium belonging to a genus Paraprevotella as an active ingredient.

The present disclosure provides methods of large-scale preparation of alpha-1 antitrypsin (AAT) activity modulators useful for treating alpha-1 antitrypsin deficiency (AATD), such as 4-(5-(4-fluorophenyl)-6-(tetrahydro-2H-pyran-4-yl)-1,5-dihydropyrrolo[2,3-f]indazol-7-yl)benzoic acid (Compound 1), 3-[5-(4-fluorophenyl)-6-isopropyl-1H-pyrrolo[2,3-f]indazol-7-yl]propanoic acid (Compound 2), or any pharmaceutically acceptable salt of any of the foregoing.

The application discloses an amino-functionalized magnetic mesoporous nanomaterial and a preparation method and application thereof, and the nanomaterial is Fe3O4@nSiO2@TiO2@mSiO2-NH2; from inside to outside, the nanomaterial comprises a magnetite magnetic core, a dense silica layer, a titanium dioxide layer and an amino-functionalized mesoporous silica layer. The magnetic core is synthesized by a solvothermal method, the dense silica layer and the titanium dioxide layer are coated by adopting a sol-gel method in sequence, and finally the amino-functionalized mesoporous silica layer is modified by a co-condensation method. The material can protonate the surface amino group to form a local alkaline microenvironment under an acidic condition, can maintain trypsin activity, realizes synchronous performance of rapid enzymolysis of proteins and in-situ enrichment of phosphorylated peptides, and significantly shortens the enzymolysis time. The application has excellent enrichment selectivity for the phosphorylated peptides, the sensitivity reaches 10 fmol / μL, the enrichment capacity is about 100 mg / g, and is suitable for high-throughput quantitative analysis of phosphorylated proteins in complex biological samples.

The present application belongs to the field of biomedical detection technology, and particularly relates to a reaction solution for detecting trypsin-like transpeptidase activity and application thereof. The present application uses BAPNA as a specific substrate, which can produce a detectable signal change in the transpeptidation reaction, and a high-concentration amino group-glycine amide is added as a receptor molecule to enhance the transpeptidation reaction efficiency. Therefore, the present application solves the technical problem that the enzyme activity cannot be detected by the immunization method, has high reaction efficiency, stable and reliable detection process, shortened operation time (within 15 minutes), simple operation, strong practicability, can be used for evaluating the severity of acute allergic reaction, providing scientific guidance for the diagnosis of mast cell-related airway inflammation and mast cell activation syndrome, monitoring the effect of allergic treatment, and has clinical value in airway inflammation typing, differential diagnosis and efficacy monitoring through induced sputum or BAL fluid trypsin activity determination.

This disclosure provides large-scale processes for preparing a modulator of alpha-1 antitrypsin (AAT) activity that may be useful for treating alpha-1 antitrypsin deficiency (AATD), such as 4-(5-(4-fluorophenyl)-6-(tetrahydro-2H-pyran-4-yl)-1,5-dihydropyrrolo[2,3-f]indazol-7-yl)benzoic acid (Compound 1), 3-[5-(4-fluorophenyl)-6-isopropyl-1H-pyrrolo[2,3-f]indazol-7-yl]propanoic acid (Compound 2), or a pharmaceutically acceptable salt of any of the foregoing.

This invention provides a method for screening, separating, and identifying trypsin inhibitors, belonging to the field of biomedical technology. The invention first uses colorimetric sensor technology to rapidly identify samples with trypsin-inhibiting activity. Then, using affinity adsorption technology combined with liquid chromatography-mass spectrometry (LC-MS), specific components in the samples with trypsin-inhibiting activity can be identified. Specifically, trypsin is immobilized on Tp-Pa to form a TRY@Tp-Pa affinity chromatography carrier material. The extract to be screened is incubated with the affinity chromatography carrier material, and after removing the unadsorbed liquid, methanol is used to elute the eluent, which contains the bioactive compounds retained on TRY@Tp-Pa. These compounds are the potential active ingredients, and LC-MS is then used to identify the components. The method provided by this invention can efficiently and accurately detect trypsin activity and screen inhibitors, with high screening efficiency and a low false positive rate.

The invention belongs to the technical field of biomedical detection, and particularly relates to reaction liquid for detecting trypsin transpeptidase activity and application of the reaction liquid. BAPNA is adopted as a specific substrate, detectable signal change can be generated in the transpeptidation reaction, then high-concentration amino group-glycinamide is added as an acceptor molecule, and the transpeptidation reaction efficiency is enhanced. Therefore, the technical problem that the enzyme activity cannot be detected by an immunoassay is solved, the reaction efficiency is high, the detection process is stable and reliable, the operation time is shortened to be within 15 minutes, the operation is simple and convenient, and the practicability is high; the kit can be used for evaluating the severity of acute anaphylaxis, provides scientific guidance for diagnosis of mast cell related airway inflammation and mast cell activation syndrome, monitors the anaphylaxis treatment effect, and has clinical value in the aspects of airway inflammation typing, differential diagnosis and curative effect monitoring by inducing phlegm or BAL liquid trypsin activity determination.

The invention provides a trypsin inhibitor and application thereof, and relates to the technical field of molecular biology. According to the invention, it is found for the first time that zinc pyrithione, ketoconazole and / or selenium disulfide have an inhibition effect on trypsin activity, and new functional components which are convenient to obtain are provided for the trypsin inhibitor. The invention provides three compositions containing zinc hyaluronate, the zinc hyaluronate is respectively compounded with zinc pyrithione, ketoconazole or selenium disulfide according to a specific mass ratio, and it is found for the first time that the zinc hyaluronate and the zinc pyrithione, ketoconazole or selenium disulfide show the activity of synergistically inhibiting trypsin. The composition provided by the invention is simple to obtain, can be continuously compounded with other functional active components or auxiliary material components to obtain commercial products, and has a wide application prospect.

This invention discloses a composite hydrogel based on sodium alginate and citric acid, its preparation method, and its applications, belonging to the field of biomedical materials and polymer hydrogels. The composite hydrogel is formed by mixing two components, A and B, to form a gel. Component A contains an aqueous solution of sodium alginate and citric acid, while component B is a crosslinking agent solution containing soluble polyvalent cations. The polyvalent cations are selected from one or more combinations of calcium ions, barium ions, and strontium ions. This invention utilizes citric acid to competitively chelate metal cations with sodium alginate, effectively slowing down the gelation rate and optimizing the three-dimensional density of the gel, significantly improving the in-situ adhesion and sealing stability of the material. Simultaneously, the local slow-release of citric acid lowers the pH of the microenvironment to a slightly acidic range of 6.0-6.5, achieving non-competitive inhibition of trypsin activity.