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Serum-free medium

A technology of serum-free medium and high content, which is applied in the field of stem cells and bioengineering, can solve the problems of being not as convenient as serum-containing medium, achieve stable and controllable quality, reduce operating procedures, and promote cell growth and division

Inactive Publication Date: 2018-01-12
SHENZHEN HORNETCORN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the current commercial serum-free medium is not as convenient to use as the serum-containing medium

Method used

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  • Serum-free medium
  • Serum-free medium
  • Serum-free medium

Examples

Experimental program
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Effect test

Embodiment 1

[0075] Serum-free medium components are: DMEM / F12, combination 1 (5X), combination 2 (2X), combination 3 (5X), combination 4 (1X), combination 5 (5X), combination 6 (10X), combination 7 ( 10X). Use this recipe for cell isolation and culture.

Embodiment 2

[0077] Serum-free medium components are: DMEM / F12, combination 1 (3X), combination 2 (1X), combination 3 (3X), combination 4 (1X), combination 5 (3X), combination 6 (6X), combination 7 ( 6X). Use this recipe for cell isolation and culture.

Embodiment 3

[0079] Serum-free medium components are: DMEM / F12, combination 1 (1X), combination 2 (1X), combination 3 (1X), combination 4 (1X), combination 5 (1X), combination 6 (2X), combination 7 ( 2X). Use this recipe for cell isolation and culture.

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Abstract

The invention discloses a serum-free medium for stem cells. The serum-free medium can be used for culturing mesenchymal stem cells. The serum-free medium for stem cells comprises DMEM / F12, cell growthfactors, hormones, proteins, vitamins and reducing substances, adherence promoting substances, trypsin inhibitors and other components. The serum-free medium of the invention is definite in added components, stable and controllable quality and batch consistency, and high safety and stability. The stem cell medium can inhibit trypsin activity, promote cell adherence and maintain the characteristics and proliferation ability of mesenchymal stem cell for a long time, and is applicable as an alternative to a serum medium.

Description

technical field [0001] The invention relates to the field of stem cells and bioengineering, in particular to a serum-free medium mainly used for stem cell cultivation. Background technique [0002] Mesenchymal stem cells (MSCs) are a type of adult stem cells with multi-directional differentiation potential, derived from various tissues and organs of the human body, including umbilical cord, placenta, bone marrow, dental pulp, peripheral blood, umbilical cord blood, and corneal limbus Wait. More and more studies have shown that MSCs can repair damaged tissues and organs, and have therapeutic effects on many diseases. Diseases that MSC can treat and achieve certain curative effect include myocardial infarction, neurological disease, spinal injury, liver cirrhosis, lupus erythematosus, etc. MSC can promote wound healing, and also has the function of supporting hematopoiesis and immune regulation. Due to the therapeutic effect of MSC on many diseases, MSC has been widely conce...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 刘聪黄馨萍陈晨魏志璋罗晓玲
Owner SHENZHEN HORNETCORN BIOTECH