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Cell Freezing Solution

A cryopreservation, cell technology, applied in the field of cell and bioengineering, can solve problems such as protein denaturation and cytotoxicity, and achieve the effects of small cell damage, consistent shape and good proliferation ability.

Active Publication Date: 2022-01-28
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the refrigerants currently used, dimethyl sulfoxide (DMSO) is the most commonly used refrigerant in cell preservation, but it also has certain toxicity to cells at the same time, and can denature intracellular proteins at room temperature. Therefore, these deficiencies limit its further application

Method used

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  • Cell Freezing Solution
  • Cell Freezing Solution
  • Cell Freezing Solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A cell cryopreservation solution, the basic component of which is RPMI-1640 medium, and the added components are as follows:

[0020] Glycerin (glycerinum) 10v / v%;

[0021] Fetal calf serum 10v / v%;

[0022] Sodium alginate 0.10w / v%.

Embodiment 2

[0024] A cell cryopreservation solution, the basic component of which is DMEM-F12 medium, and the added components are as follows:

[0025] Glycerin 10v / v%;

[0026] Fetal bovine serum 10v / v%;

[0027] Sodium Alginate 0.01w / v%.

Embodiment 3

[0029] A cell cryopreservation solution, the basic component of which is RPMI-1640 medium, and the added components are as follows:

[0030] Glycerin 10v / v%;

[0031] Fetal bovine serum 10v / v%;

[0032] Sodium hyaluronate (Hyaluronic acid sodium salt) 0.10w / v%.

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Abstract

The cell cryopreservation solution of the present invention contains basal medium, glycerin, fetal bovine serum and non-permeable protective agent, and the content of each component is: 80-94v / v% of basal medium, 5-10v / v% of glycerin, Fetal bovine serum 1~10v / v%, non-permeable protective agent 0.01~0.10w / v%; the basal medium is RPMI-1640, MEM, high-sugar DMEM, low-sugar DMEM or DMEM-F12; the non-permeable The osmotic protectant is sodium alginate, sodium hyaluronate or γ-polyglutamic acid; the present invention does not contain dimethyl sulfoxide, but is obtained through different combinations of non-osmotic protectant, glycerin and fetal bovine serum Good freezing effect, strong water molecule complexing ability, similar to dimethyl sulfoxide in freezing function, but can reduce the formation of ice crystals in cells during freezing; as a cell freezing solution, the added ingredients are clear and safe High stability, little damage to cells, consistent shape of cells before and after cryopreservation, high cell survival rate after recovery, and good proliferation ability.

Description

technical field [0001] The invention relates to the technical field of cells and bioengineering, in particular to a cell cryopreservation solution. Background technique [0002] Cell cryopreservation is one of the important links in biological research. It freezes artificially cultured cells, prevents cell degradation caused by cell subculture, prevents bacterial contamination in subculture, and solves a series of problems in the process of maintaining subculture. Questions matter. However, cryopreservation of cells can also damage cells to a certain extent. [0003] At present, in the field of cell cryopreservation, cryogens (English full name Cryo-Protectant Agent, CPA for short) are widely used. Described refrigerant comprises osmotic refrigerant and non-permeable refrigerant, and wherein, osmotic refrigerant mainly comprises dimethyl sulfoxide, glycerin, ethylene glycol, propylene glycol, acetamide and methyl alcohol; Its protection mechanism is: in Before the cell fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 蔡海波陈焕芸周乐豪谭文松
Owner EAST CHINA UNIV OF SCI & TECH
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