The invention relates to a health-care wine with nourishing yin, tonifying yang, and regulating immune function. The health-care wine with the alcohol content of 9-15 % is prepared by mixing millet, glutinous rice, rice, preserved rhizoma polygonati, rehmannia glutinosa, radix rehmanniae, cistanche deseuticola ma, polygonum multiflorum thumb, lucid ganoderma, wolfberry, cuscuta chinensis, fructuspsoraleae, fructus corni, longan, cynomorium songaricum, common curculigo rhizome, epimeddium, radix achyranthis bidentatae, and semen coicis according to a certain proportion, and carrying out digestion, fermentation and filtering. According to the health preserving theory of Taoism, and by combining the principle of mutual generation and restriction of traditional Chinese medicines, the medicines are efficiently combined to cause the efficiency of the medicines to generate synergistic effects, the beneficial components of the medicines are released completely by digestion and fermentation, the medicines go to work with the wine properties, and the wine go to work with the principles of the medicines, so that the medicines and the wine go to work in health care effects of nourishing liver, tonifying kidney, regulating spleen and stomach, tonifying Yin and Yang, improving immunity, slowing down ageing, maintaining beauty, removing beverage, relaxing the muscles, stimulating the blood circulation and the like together. Simultaneously, the invention has the advantages of simple process and low cost and is favorable to popularization.