Porcine epidemic diarrhea virus, and culture method and application thereof

A technology for porcine epidemic diarrhea and viruses, applied in the direction of antiviral agents, viruses/bacteriophages, and microbial-based methods, which can solve the problems of limited selection and lack of broad immunogenicity

Active Publication Date: 2014-04-30
WENS FOODSTUFF GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, because the vaccines produced by several domestic manufacturers are all PEDV CV777 strains isolated earlier (referred to as CV777 strains in this invention), they lack a wide range of immunogens nature, the range of choices in production practice is very limited

Method used

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  • Porcine epidemic diarrhea virus, and culture method and application thereof
  • Porcine epidemic diarrhea virus, and culture method and application thereof
  • Porcine epidemic diarrhea virus, and culture method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1 GDSJ / 2012 virus strain obtains

[0031] 1. Collection and processing of disease materials

[0032] Seven fresh feces of piglets with suspected viral diarrhea in a pig farm in Guangdong Province were collected, diluted with PBS (phosphate buffer saline) at a weight-to-volume ratio of 1:10 after numbering, shaken and mixed evenly, and repeatedly frozen and thawed 3 times , centrifuged at 8000r / min (rotation per minute) for 10 min (minutes), took the supernatant, sterilized it with a filter with a pore size of 0.22 μm (microns), and stored the resulting treated solution in a -70°C refrigerator for later use.

[0033] 2. RT-PCR (reverse transcription polymerase chain amplification reaction) detection of disease materials

[0034] Take 200 μL (microliter) of each of the above treatment solutions numbered 1-7, and use the AxyPrep Body Fluid Virus DNA / RNA Mini Kit produced by Shanghai Baisai Biotechnology Co., Ltd. to extract RNA. The extracted RNA was reverse-t...

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Abstract

The invention discloses a porcine epidemic diarrhea virus, and a culture method and an application thereof. The porcine epidemic diarrhea virus is called as coronaviridae coronavirus porcine epidemic diarrhea virus GDSJ / 2012, is preserved in China center for type culture collection on May 15th, 2013, and has the preservation number of CCTCC NO:V201309. The culture of the virus strain requires a serum-free DMEM culture solution containing trypsin and magnesium chloride. The virus strain can be used for preparation of a PEDV diagnostic kit and a PEDV vaccine, has good immunogenicity, and can make up for the deficiency of few conventional vaccine types.

Description

technical field [0001] The invention relates to the field of microbial viruses, in particular to a new strain of porcine epidemic diarrhea virus, its cultivation method and application. Background technique [0002] Porcine Epidemic Diarrhea Virus (PEDV) is the pathogen of porcine intestinal infectious disease that causes vomiting, diarrhea and dehydration as the main clinical symptoms of pigs (Yin Zhen, 1997; B. E. Straw, 2000), different age and Pigs of different breeds are susceptible to infection, but the hazard to suckling piglets is the most serious, with a mortality rate of up to 100%, causing huge economic losses to the pig industry. It is one of the key diseases in the current prevention and control of pig infectious diseases in my country. [0003] At present, there is no specific drug for treating porcine epidemic diarrhea, and the effect of conventional treatment is not good. Therefore, vaccine prevention is still the main method, and the success of the virus stra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00G01N33/569A61K39/215A61P31/14C12R1/93
Inventor 田小艳宋延华周庆丰潘永飞
Owner WENS FOODSTUFF GRP CO LTD
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