A strain of porcine epidemic diarrhea virus and its culture method and application

A technology for porcine epidemic diarrhea and viruses, applied in the direction of antiviral agents, viruses/bacteriophages, and microbial-based methods, which can solve the problems of limited selection and lack of broad immunogenicity
CN103756974BActive Publication Date: 2016-08-10WENS FOODSTUFF GRP CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WENS FOODSTUFF GRP CO LTD
Publication Date
2016-08-10

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Abstract

The invention discloses a strain of porcine epidemic diarrhea virus and its cultivation method and application. The strain of porcine epidemic diarrhea virus is named Coronaviridae Coronaviridae Porcine Epidemic Diarrhea Virus GDSJ / 2012 (Porcine epidemic diarrhea virus). It was deposited in the China Center for Type Culture Collection on May 15, 2013, with the preservation number CCTCC NO:V201309. The culture of this strain requires the use of serum-free DMEM medium containing trypsin and magnesium chloride. It can be used to prepare PEDV diagnostic reagents and PEDV vaccines, has good immunogenicity, and can make up for the shortage of existing vaccines.
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Description

technical field

[0001] The invention relates to the field of microbial viruses, in particular to a new strain of porcine epidemic diarrhea virus, its cultivation method and application. Background technique

[0002] Porcine Epidemic Diarrhea Virus (PEDV) is the pathogen of porcine intestinal infectious disease that causes vomiting, diarrhea and dehydration as the main clinical symptoms of pigs (Yin Zhen, 1997; B. E. Straw, 2000), different age and Pigs of different breeds are susceptible to infection, but the hazard to suckling piglets is the most serious, with a mortality rate of up to 100%, causing huge economic losses to the pig industry. It is one of the key diseases in the current prevention and control of pig infectious diseases in my country.

[0003] At present, there is no specific drug for treating porcine epidemic diarrhea, and the effect of conventional treatment is not good. Therefore, vaccine prevention is still the main method, and the success of the virus stra...

Claims

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