sgRNA sequence for knocking out human CYP2E1, construction method of deficiency cell strain of CYP2E1 and application thereof
A technology of gene deletion and construction method, applied in the field of genetic engineering, can solve problems such as inability to gene, incomplete gene expression silencing, and inability to construct CYP2E1 gene-deficient cell lines, etc., to achieve silencing, improve incomplete silencing or inability to silence The effect of gene expression
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[0029] The following description is a preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered Be the protection scope of the present invention.
[0030] Unless otherwise specified in the examples of the present invention, all reagents and consumables used are commercially available.
[0031] The technical solution of the present invention can be realized through the following embodiments:
[0032] (1) sgRNA design:
[0033] The sgRNA sequences were designed for the second, third and seventh exons (Exon2, Exon3, Exon7) of CYP2E1, respectively.
[0034] The specific grouping and naming of the three groups of designed and synthesized sgRNA sequences targeting the second, third and seventh exons of CYP2E1 are shown in Table 1:
[0035] Table 1 CYP2E1 sg...
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