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Rice plant type relevant gene RL10 and use thereof

A rice and gene technology, applied in the field of genetics and breeding, to achieve the effects of stable growth, improved photosynthesis efficiency and compact plant shape

Inactive Publication Date: 2009-03-18
YANGZHOU UNIV
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  • Claims
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Problems solved by technology

But so far there is no report about the regulation of leaf polarity by F-box protein

Method used

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  • Rice plant type relevant gene RL10 and use thereof
  • Rice plant type relevant gene RL10 and use thereof
  • Rice plant type relevant gene RL10 and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 0. Acquisition of materials

[0024] In 1999, using isotopic 60 Co irradiated Zhonghua 11 mature seeds, and obtained 2 curl-leaf mutants in the M1 generation, named rl10-1 and rl10-2 respectively. Compared with the wild-type (WT) variety Zhonghua 11, this mutant has curled leaves, more grains per panicle, thicker stems, and straighter flag leaves ( figure 1 ). .

[0025] 1 Preliminary mapping of genes

[0026] F with rl10-1×Dular 2 group as the initial positioning group. Since in this F 2 There were some individuals between the parents in the population, so another symbolic trait of the mutant—dense panicle—was taken into account when sampling, that is, the selected single plant had both curly leaf and dense panicle as indicators. rl10-1×Dular F 2 There were a total of 200 plants in the population, and 27 individual plants with similar phenotypes to the mutants and accurate traits were collected, and these 27 recessive individuals formed a preliminary positionin...

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Abstract

The invention belongs to the field of genetics and breeding, in particular relates to a rice plant type related gene RL10 and application thereof. The rice gene RL10 has a sequence as shown in SEQ ID NO.1, and the rice gene RL10 codes F-box proteins and controls the polar growth of rice leaves and the morphogenesis of rice panicle traits. The gene can improve the plant types and the panicle types of the prior species, improve the yield, and has wide application prospect in the high-yield breeding of rice.

Description

technical field [0001] The invention belongs to the field of genetic breeding, and in particular relates to a rice plant type-related gene RL10 and an application thereof. Background technique [0002] The discovery of excellent rice genetic resources is the material basis for super-high-yield breeding, and the discovery of the functions of genes that control rice plant type and yield has an important role in promoting rice breeding. In this study, a material (rl10) with mutations in plant type and yield traits was screened in the mutant library created by ourselves, and the Rl10 gene was cloned using the map-based cloning strategy, and the coding sequence of the gene was obtained. The cloning and functional analysis of this gene is reported for the first time in rice. It not only plays an important role in rice development, but also has important utilization value in high-yield breeding of rice. [0003] F-box protein contains F-box motif (motif), which is a subunit of SCF...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415A01H1/00A01H5/00
Inventor 严长杰顾铭洪程祝宽严松
Owner YANGZHOU UNIV
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