Stem cells preparation for repairing wound surface and preparation method thereof

A stem cell preparation and stem cell technology, applied in the field of biological preparations, can solve the problems of animal-derived safety, hidden dangers, and people who are not suitable for antibiotic allergies, etc., and achieve the effects of promoting epidermal cell growth, shortening wound healing time, and prolonging cell survival time

Inactive Publication Date: 2012-09-19
BEIJING HEALTH & BIOTECH STEM CELL INST CO LTD
View PDF4 Cites 34 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult for a simple cell suspension to adhere to the wound surface and continue to function, and the number of living cells in the simple cell suspension decreases with the prolongation of the medication time, which cannot guarantee enough living cells to play a therapeutic role.
[0005] The invention patent with the application number 200810136415.6 discloses a preparation method of a human umbilical cord mesenchymal stem cell wound varnish using methylcellulose as a cell matrix, but the invention contains the antibiotic gentamicin, which is not suitable for people allergic to antibiotics; Moreover, the rapid preparation of the smear contains fetal bovine serum, an animal-derived ingredient, which may cause allergies and have animal-derived safety hazards; the rapid preparation has many steps and complicated processes, which brings the risk of microbial contamination, and cannot be completed in accordance with the requirements of the Pharmacopoeia before transportation. 2 Weekly sterility testing, product quality cannot be guaranteed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Stem cells preparation for repairing wound surface and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Preparation of stem cell preparation of the present invention

[0035] Preparation of cell suspension: use DMEM medium (complete medium) containing 10% fetal bovine serum at 37°C, 5% CO 2 Rabbit umbilical cord mesenchymal stem cells were routinely cultured under the above conditions, and when the cell confluence reached about 90%, they were digested with 0.25% trypsin, and then stopped with the above-mentioned complete medium, and the resulting cell suspension was centrifuged and discarded. Supernatant, and then use serum-free DMEM medium to suspend the cell pellet, and adjust the content of rabbit umbilical cord mesenchymal stem cells in the cell suspension to contain 1.5×10 7 Rabbit umbilical cord mesenchymal stem cells, prepared into a uniform cell suspension for use;

[0036] Gel preparation: Weigh 10 g of sodium alginate, autoclave at 121°C for 15 minutes, and then add it to 100 ml of serum-free medium containing 10% DMSO and 5% propylene glycol to pr...

Embodiment 2

[0039] Embodiment 2 Preparation of stem cell preparation of the present invention

[0040] Preparation of cell suspension: use DMEM medium (complete medium) containing 10% fetal bovine serum at 37°C, 5% CO 2 Rabbit umbilical cord mesenchymal stem cells were routinely cultured under certain conditions. When the cell confluence reached about 90%, they were digested with 0.25% trypsin, and then stopped with the above-mentioned complete medium, and the resulting cell suspension was centrifuged. Discard the supernatant, then suspend the cell pellet with serum-free DMEM medium, adjust the content of rabbit umbilical cord mesenchymal stem cells in the cell suspension to 3×106 Rabbit umbilical cord mesenchymal stem cells, prepared into a uniform cell suspension for use;

[0041] Gel preparation: Weigh 10 g of sodium alginate, autoclave at 121°C for 15 minutes, and then add it to 100 ml of serum-free medium containing 10% DMSO and 5% propylene glycol to prepare a uniform sodium alginat...

Embodiment 3

[0044] Embodiment 3 Preparation of stem cell preparation of the present invention

[0045] Preparation of cell suspension: use DMEM medium (complete medium) containing 10% fetal bovine serum at 37°C, 5% CO 2 Rabbit umbilical cord mesenchymal stem cells were routinely cultured under certain conditions. When the cell confluence reached about 90%, they were digested with 0.25% trypsin, and then stopped with the above-mentioned complete medium, and the resulting cell suspension was centrifuged. Discard the supernatant, then suspend the cell pellet with serum-free DMEM medium, adjust the content of rabbit umbilical cord mesenchymal stem cells in the cell suspension to 7.5×10 7 Rabbit umbilical cord mesenchymal stem cells, prepared into a uniform cell suspension for use;

[0046] Gel preparation: Weigh 5g of sodium alginate, autoclave at 121°C for 15 minutes, and then add it to 100ml of serum-free medium containing 10% DMSO and 5% glycerol to prepare a uniform sodium alginate gel s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a stem cells preparation for repairing a wound surface and preparation method thereof, wherein the stem cells preparation uses umbilical cords, placentae or mesenchymal stem cells between amniotic membranes as living cells, and is added with high polymer material sodium alginate with excellent biocompatibility, sodium hyaluronate, chitosan and hydroxyethyl starch as cell stabilizer. The stem cells preparation for repairing the wound surface has higher cell viability and is durable in action, and breaks through technical limitations of effectiveness of the mesenchymal stem cells for treating.

Description

technical field [0001] The invention relates to the field of biological preparations, in particular to a stem cell preparation for wound repair and a preparation method thereof. Background technique [0002] It has been reported that wound coverage after severe trauma, extensive burns, and plastic surgery has become a very important issue. In addition to autologous skin grafts, people have used allogeneic skin grafts and other related repair materials to cover wounds, but Both autologous and allogeneic skin transplantation are limited by donor source and immune rejection. It is urgent to find an ideal natural medicine without rejection, promoting skin damage repair, and promoting skin regeneration. [0003] Mesenchymal stem cells (MSCs) are a group of heterogeneous cell populations derived from stroma, which can be obtained from most tissues of the human body. A large number of experimental studies have shown that mesenchymal stem cells have the potential to differentiate ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/44A61K35/50A61K9/00A61K47/36A61P17/02A61K35/51
Inventor 韩忠朝耿洁梁璐
Owner BEIJING HEALTH & BIOTECH STEM CELL INST CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap