Stem cells preparation for repairing wound surface and preparation method thereof
A stem cell preparation and stem cell technology, applied in the field of biological preparations, can solve the problems of animal-derived safety, hidden dangers, and people who are not suitable for antibiotic allergies, etc., and achieve the effects of promoting epidermal cell growth, shortening wound healing time, and prolonging cell survival time
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Embodiment 1
[0034] Embodiment 1 Preparation of stem cell preparation of the present invention
[0035] Preparation of cell suspension: use DMEM medium (complete medium) containing 10% fetal bovine serum at 37°C, 5% CO 2 Rabbit umbilical cord mesenchymal stem cells were routinely cultured under the above conditions, and when the cell confluence reached about 90%, they were digested with 0.25% trypsin, and then stopped with the above-mentioned complete medium, and the resulting cell suspension was centrifuged and discarded. Supernatant, and then use serum-free DMEM medium to suspend the cell pellet, and adjust the content of rabbit umbilical cord mesenchymal stem cells in the cell suspension to contain 1.5×10 7 Rabbit umbilical cord mesenchymal stem cells, prepared into a uniform cell suspension for use;
[0036] Gel preparation: Weigh 10 g of sodium alginate, autoclave at 121°C for 15 minutes, and then add it to 100 ml of serum-free medium containing 10% DMSO and 5% propylene glycol to pr...
Embodiment 2
[0039] Embodiment 2 Preparation of stem cell preparation of the present invention
[0040] Preparation of cell suspension: use DMEM medium (complete medium) containing 10% fetal bovine serum at 37°C, 5% CO 2 Rabbit umbilical cord mesenchymal stem cells were routinely cultured under certain conditions. When the cell confluence reached about 90%, they were digested with 0.25% trypsin, and then stopped with the above-mentioned complete medium, and the resulting cell suspension was centrifuged. Discard the supernatant, then suspend the cell pellet with serum-free DMEM medium, adjust the content of rabbit umbilical cord mesenchymal stem cells in the cell suspension to 3×106 Rabbit umbilical cord mesenchymal stem cells, prepared into a uniform cell suspension for use;
[0041] Gel preparation: Weigh 10 g of sodium alginate, autoclave at 121°C for 15 minutes, and then add it to 100 ml of serum-free medium containing 10% DMSO and 5% propylene glycol to prepare a uniform sodium alginat...
Embodiment 3
[0044] Embodiment 3 Preparation of stem cell preparation of the present invention
[0045] Preparation of cell suspension: use DMEM medium (complete medium) containing 10% fetal bovine serum at 37°C, 5% CO 2 Rabbit umbilical cord mesenchymal stem cells were routinely cultured under certain conditions. When the cell confluence reached about 90%, they were digested with 0.25% trypsin, and then stopped with the above-mentioned complete medium, and the resulting cell suspension was centrifuged. Discard the supernatant, then suspend the cell pellet with serum-free DMEM medium, adjust the content of rabbit umbilical cord mesenchymal stem cells in the cell suspension to 7.5×10 7 Rabbit umbilical cord mesenchymal stem cells, prepared into a uniform cell suspension for use;
[0046] Gel preparation: Weigh 5g of sodium alginate, autoclave at 121°C for 15 minutes, and then add it to 100ml of serum-free medium containing 10% DMSO and 5% glycerol to prepare a uniform sodium alginate gel s...
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