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Oriental lily tissue culture detoxicating method

A technology of tissue culture and lily, which is applied in the field of plant cultivation, can solve the problems of difficulty in ensuring no pollution, low efficiency, low survival rate, etc., and achieve the effect of significant detoxification effect and high efficiency

Inactive Publication Date: 2008-04-09
云南格桑花卉有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detoxification treatment of lily at home and abroad is to use stem tip, medicine or heat treatment alone. These methods have low efficiency and poor detoxification effect, and most of the detoxification rates are below 30%.
In recent years, there has been a method of cutting the shoot tip after treating the buds with hot water (50±1)°C for 40 minutes. Although a certain effect has been obtained, the tissue culture balls survived after being treated at a high temperature of 50°C for 40 minutes. At the same time, because the bulbils are not sterilized, it is difficult to ensure that they are not polluted after heat treatment and removal of the shoot tip.
So far, there are no method reports that can effectively overcome the above problems in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] In September, select a robust oriental lily plant, take out the bulbs, separate the scales from the bulbs, wash and disinfect them with clean water, and cut the scales into 3 to 5 small pieces under aseptic conditions. Put it on the growth medium for sterile culture, set the culture temperature at 23.5°C, and the light intensity at 2000-3000lx, and induce adventitious buds after 60 days of cultivation; transfer the adventitious buds to the growth medium, and place them in the artificial climate incubator Medium-variable temperature culture for 30 days, the temperature-variable culture is to cultivate 12 hours at a temperature of 37°C and a light intensity of 2000-3000lx every day, and cultivate at a temperature of 22°C and a dark environment for 12 hours, and the two are carried out alternately; after 30 days After the day-variable temperature treatment, under aseptic conditions, cut off the 0.8mm shoot tip, transplant it into the growth medium and continue the temperatu...

Embodiment 2

[0025] Repeat Example 1 with the following difference: the cut scales are placed at 22° C. for cultivation.

Embodiment 3

[0027] Repeat Example 1 with the following difference: the cut scales are placed at 25° C. for cultivation.

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PUM

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Abstract

The invention discloses an orient lily tissue culture detoxification method, and selects healthy lily and cut into squamas, induces adventitious buds; to transplant the adventitious buds to the growth substrate, after 30 days cultivation, to cut off the stem tip and transplant in the growth substrate for variable temperatures cultivation for 30-60 days again, and then the needed nontoxic plant series is obtained. The invention has obvious detoxification effect, high efficiency which can reach 90-100 percent commonly. With simple operation and low cost, and thereby the invention has bright application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a detoxification method for oriental lily tissue culture. Background technique [0002] Oriental lily (Lilium spp.cv.oriental hybrids) is a hybrid strain in the genus Lilium, and it is one of the most popular flower bulbs in the international market. Due to the huge market demand, my country imports a large number of oriental lily flower bulbs and planting female parents from abroad every year. Taking 2006 as an example, about 70 million flowering bulbs were imported and about 8 million female plants were planted. It not only consumes a lot of foreign exchange, but also increases the operating costs and risks of the enterprise. [0003] At present, the artificial cultivation of oriental lily seedlings adopts the conventional plant tissue culture method, that is, the scales are used as explants, the callus is first induced, and after the transfer, the callus...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 丁元明熊灿坤熊劲和莲彩寸东义杨红梅和捷
Owner 云南格桑花卉有限责任公司
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