Method for cultivating and producing detoxic seedling on strawberry stemp apex

A technology of virus-free seedlings and strawberry stolons, which is applied in the field of agricultural seedlings, can solve problems such as variation, strawberry seedlings cannot maintain seed properties, and do not meet production needs, and achieve the effects of reduced virus particles, low probability of mutation, and improved quality

Inactive Publication Date: 2006-03-22
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

The disadvantage of this technology is that the use of leaves to establish an asexual reproduction system must go through the stage of callus, which will inevitably produce a large number of variations. Although the regeneration rate is high, the cultivated strawberry seedlings cannot maintain their species, which is not in line with Production needs

Method used

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Embodiment Construction

[0013] Provide following embodiment in conjunction with content of the present invention, concrete implementation is as follows:

[0014] 1. Sterilization of materials: Select the 5cm-long terminal buds from the top of the stolons, which are well grown and the leaflets are still unfolded, from the strong strawberry mother plant in the field. After washing with tap water for 2 hours, they are sterilized on an ultra-clean bench. Remove the large outer leaves of the stolons with tweezers, then quickly treat them with 70% alcohol for 30 seconds, soak them in saturated bleaching semen for 15 minutes, and then rinse them three times with sterile water.

[0015] 2. Shoot tip culture Peel off the young leaves layer by layer from outside to inside under a binocular dissecting microscope until the top meristem of the shiny hemisphere is fully exposed, cut a 0.2mm size shoot tip and inoculate it into the pre-prepared MS+BA0 .5mg / L culture medium, the culture container is a 100ml glass bo...

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Abstract

The present invention belongs to the field of agricultural seedling culturing technology. The strawberry stem apex culturing process to produce detoxicated seedling includes the following steps: 1. taking strawberry terminal bud in spring or autumn and sterilizing; 2. taking stem apex of 0.2 mm size under binocular anatomical lens and culturing in compounded 0.5 mg / L concentration MS+BA culture medium to grow cespitose bud; 3. inoculating cespitose bud to 0.2 mg / L concentration MS+BA culture medium for culturing; 4. cutting down giant strawberry seedling from the cespitose bud after proliferation culture, and transferring to 1 / 2MS culture medium for rooting while culturing the rest in the 0.2 mg / L concentration MS+BA culture medium; and 5. domesticating test tube seedling via transplanting the rooted seedling to seedling bed. The said process has 30 % raised detoxicated seedling producing efficiency and obviously improved quality compared with convenient seedling culturing process.

Description

technical field [0001] The invention relates to a method in the technical field of agricultural seedling cultivation, in particular to a method for producing virus-free seedlings by cultivating strawberry stem tips. Background technique [0002] Strawberry (Fragaria ananassa Ducde) is a perennial evergreen herbaceous plant of the genus Fragaria in the family Rosaceae. It is a crop that reproduces asexually by stolons. The accumulation of viruses in the body increases year by year, and serious viral diseases occur. Strawberries infected with the virus grow slowly, with shrunken leaves and smaller, deformed, and poor-quality fruits year after year, generally reducing production by 30%-80%, and increasing year by year. Strawberry virus disease that has caused loss in my country mainly contains four kinds such as strawberry mottle virus (SmoV), strawberry mild yellow edge virus (SMYEV), strawberry vein virus (SVBV), strawberry shrunken virus (SCrV). It is reported that the tota...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 何欢乐蔡润潘俊松
Owner SHANGHAI JIAO TONG UNIV
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