Tissue cultivation rapid breeding method of spring dendrobium stem
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A technology of tissue culture and spring dendrobium, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of low seedling rate and obvious quality difference
Active Publication Date: 2008-05-21
ZHEJIANG SENHE SEED
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Problems solved by technology
The traditional cutting propagation technology of Dendrobium dendrobii has a low seedling rate and obvious quality differences, so the propagation technology of Dendrobium dendrobium is facing huge pressure from factory cultivation
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[0025] The spring dendrobium tissue culture rapid propagation method is characterized in that comprising the following steps:
[0026] 1) Select spring dendrobium stem tips with good variety characteristics and robust growth as explants, and the length of the young stems is 3-5cm.
[0027] 2) Processing of explants: peel off the leaves on the outer nodes of the young stems, soak or scrub the young stems with leaves and leaf sheaths with 70-75% alcohol after cleaning, then peel off the lower leaves and leaf sheaths, and leave the package The 1-2 leaves of the stem tip are soaked in 0.1-0.15% mercury liter (add 2-3 drops of Tween 20) for 6-8 minutes, then soaked in 8-10% NaClo solution for 15-20 minutes, and finally use Wash with distilled water 4-5 times until there is no peculiar smell.
[0028] 3) Cut off the cut surface of the explant that has been in contact with the disinfectant. The blade must be kept sharp. In the sterile inoculation room, use the knife back extrusion m...
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Abstract
A fast propagation method of tissue culture of Dendrobium primulinum Lindl belongs to the technical field of plant fast propagation. The method is characterized by comprising the steps: 1) a young stem of the Dendrobium primulinum Lindl with a length of 3 to 5cm is chosen as an explant; 2) the treating of the explant; 3) inoculation and culture for 10 to15 days in an aseptic inoculation chamber; 4) a survived stem tip is transferred to a inducing medium for inducing an adventitious bud or a protocorm which, after 30 to 40 days, is transferred to a reproducing medium for reproducing of 2 to 3 months; 5) successive transfer and rooting culture; 6) a single plant is transplanted in a 128 plug-tray. The fast propagation method of tissue culture of the Dendrobium primulinum Lindl is not affected by seasons. Due to the easy controlled propagation environment, the Dendrobium primulinum Lindl adapts to various places of China. Therefore, the problems of hard-breeding, easy degeneration, quick variation and irregular seedling quality of the Dendrobium primulinum Lindl are resolved. Seedlings of the Dendrobium primulinum Lindl with good and uniform quality are produced and have wide market prospect.
Description
technical field [0001] The invention belongs to the technical field of rapid propagation methods for plants, in particular to a rapid propagation method for dendrobium dendrobium tissue culture. Background technique [0002] Spring Dendrobium (Dendrobium), orchidaceae (Orchidaceae) Dendrobium genus, perennial herb, epiphytic, stems clustered, noded, yellow-green, leaves nearly leathery, ovate-lanceolate, 9-12cm long, racemes. There are 76 native species in my country, mainly distributed in Yunnan, Guangdong, Guangxi, Taiwan, Hainan and other places. Dendrobium orchids in Japan, Thailand, Singapore and other countries are very mature in industrialization scale, and the breeding work is outstanding, and a wealth of hybrid varieties have been cultivated. The flower colors are red, white, yellow and various composite colors. Production of high-grade flowers. Dendrobium hybrids are mainly divided into three categories: Dendrobium nobile, Dendrobium formidible, and Dendrobium ph...
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