Method for cryopreservation and plant regeneration of Dianthus caryophyllus shoot tip

Abstract

The invention provides a method for cryopreservation and plant regeneration of Dianthus caryophyllus shoot tips. Growth-restored regenerated Dianthus caryophyllus plants are obtained through preculture, loading, dehydration, freezing, thawing, restoration culture and the like. By adopting the method provided by the invention, the regeneration rate of Dianthus caryophyllus can reach 52.4 percent, the device for the vitrification cryopreservation of the Dianthus caryophyllus shoot tips is simple, the process is simplified, the operation is easy, the freezing effect is good, the restoration growth of the preserved Dianthus caryophyllus is good, the hereditary variation probability is small and the method is an effective way for the in vitro preservation of Dianthus caryophyllus germ plasm resources.

Description

technical field

[0001] The invention relates to a method for preserving plant germplasm resources, in particular to a method for ultra-low temperature preservation of carnation stem tip vitrification, and belongs to the technical field of plant cell engineering. Background technique

[0002] Carnation (Dianthus caryophyllus), also known as carnation, is a perennial herbaceous plant of the genus Caryophyllum in the family Caryophyllaceae. It is one of the world-renowned fresh cut flowers and one of the most important bulk export flowers in Yunnan Province.

[0003] At present, the preservation of carnation germplasm resources mainly adopts the field preservation method and the tissue culture preservation method. The field preservation method is vulnerable to the invasion of diseases and insect pests and the threat of harsh environment. Avoidance of changes in certain genetic traits.

[0004] Cryopreservation is an ideal way to preserve plant germplasm. Since Nag and Street f...