Growth-limiting preservation method for potato test-tube plantlets

A technology for limiting growth and preservation and test-tube seedlings, which is applied in the field of limiting growth and preservation of potato test-tube seedlings, can solve problems such as stunted plant growth and abnormal growth, and achieve the effects of restoring good growth, low consumption, and avoiding the loss or destruction of germplasm.

Active Publication Date: 2021-12-28
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that adding growth inhibitors to the medium can prolong the storage time for 12 months, but long-term use of growth inhibitors may cause stunted plant growth and abnormal growth, and may also lead to material resistance or tolerance to growth inhibitors choice of force

Method used

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  • Growth-limiting preservation method for potato test-tube plantlets
  • Growth-limiting preservation method for potato test-tube plantlets
  • Growth-limiting preservation method for potato test-tube plantlets

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A kind of potato test-tube seedling restriction growth preservation method, concrete steps are as follows:

[0026] (1) Tissue culture: select respectively 1 week, 2 weeks and 3 weeks of potato sterile test-tube plantlets, and place the above-mentioned potato sterile test-tube plantlets at a culture temperature of 20-25°C, light intensity of 3000-4000lx, and photoperiod Under the condition of 16h / darkness for 8h, insert into MS+30g / L sucrose+8g / L agar (pH5.8) medium and culture for 1 week respectively;

[0027] (2) Low-temperature exercise: In step (1), the aseptic test-tube plantlets of vigorously grown potatoes were randomly and equally divided into 3 groups according to the same seedling age, a total of 9 groups; respectively inserted into special storage bags, and the low-temperature exercise medium was 1 / 2MS+30g / L sucrose+8g / L agar (pH5.8), each seedling-age potato sterile test-tube plantlets were respectively cultured according to grouping conditions at 20°C for ...

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Abstract

The invention relates to the technical field of in-vitro test-tube plantlet preservation, and discloses a growth-limiting preservation method for potato test-tube plantlets, which comprises the following steps: (1) tissue culture: inoculating a culture medium with potato sterile test-tube plantlets for culture for 1 week under the conditions that the culture temperature is 20-25 DEG C, the illumination intensity is 3000-4000 lx, and the photoperiod is 16 h of illumination / 8 h of darkness; (2) low-temperature exercise: selecting the potato sterile test-tube plantlets which grow robustly in the step (1), inoculating special preservation bags with the potato sterile test-tube plantlets, and performing low-temperature exercise for 1-2 weeks under the conditions that the culture temperature is 20 DEG C, illumination is performed for 8 h , and darkness is performed for 16 h at 4 DEG C; and (3) growth limiting: selecting the potato sterile test-tube plantlets which grow robustly in the step (2), inoculating special preservation bags with the potato sterile test-tube plantlets, and preserving the potato sterile test-tube plantlets at 4 DEG C without illumination; The preservation method is based on a tissue culture technology, the preservation period of the potato test-tube plantlets can reach 2 years, and the technology which is low in maintenance, low in consumption and longer and is suitable for medium and long-term preservation of potatoes is formed.

Description

technical field [0001] The invention relates to the technical field of preservation of isolated test-tube plantlets, in particular to a method for limiting growth and preservation of potato test-tube plantlets. Background technique [0002] Potato (Solanum tuberosum L.) is an annual dicotyledonous herbaceous plant of Solanaceae, Solanaceae. There are more than 2,000 species in total. Originated in the Andes plateau region of countries such as Peru and Bolivia in Latin America, it is the crop that is grown and eaten in the most countries in the world, and it is also the fourth largest food crop in the global economy after rice, wheat and corn. In the 16th century, Spanish and British explorers brought potatoes from Latin America back to their home countries for planting, and then spread them to Italy and various parts of Europe. Potato has a history of more than 400 years of cultivation in my country, but the germplasm resources of potato in China are relatively limited. Po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 张金梅陈晓玲辛霞卢新雄尹广鹍何娟娟
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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