A kind of potato plantlet growth restriction preservation method

A technology for limiting growth and preservation and test-tube seedlings, which is applied to the field of limiting growth and preservation of potato test-tube seedlings, can solve problems such as abnormal growth and poor plant development, and achieves the effects of restoring good growth, low consumption, and reducing the frequency of subgenerations.

Active Publication Date: 2022-06-17
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that adding growth inhibitors to the medium can prolong the storage time for 12 months, but long-term use of growth inhibitors may cause stunted plant growth and abnormal growth, and may also lead to material resistance or tolerance to growth inhibitors choice of force

Method used

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  • A kind of potato plantlet growth restriction preservation method
  • A kind of potato plantlet growth restriction preservation method
  • A kind of potato plantlet growth restriction preservation method

Examples

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Embodiment 1

[0025] A method for limiting growth and preservation of potato test-tube seedlings, the specific steps are as follows:

[0026] (1) Tissue culture: select aseptic test-tube potato seedlings with seedling ages of 1 week, 2 weeks and 3 weeks respectively, and culture the above-mentioned sterile test-tube potato seedlings at a culture temperature of 20-25°C, a light intensity of 3000-4000 lx, and a photoperiod of light. Under the conditions of 16h / darkness for 8h, the cells were cultured in MS+30g / L sucrose+8g / L agar (pH5.8) medium for 1 week respectively;

[0027] (2) Low temperature exercise: in step (1), the robust potato sterile test tube seedlings were randomly and equally divided into 3 groups according to the same seedling age, totaling 9 groups; respectively connected to special storage bags, and the low temperature exercise medium was 1 / 2MS+30g / L sucrose + 8g / L agar (pH 5.8), each seedling-age potato aseptic test-tube plantlets were cultured at 20°C for 8h (light intens...

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Abstract

The invention relates to the technical field of isolated test-tube seedling preservation, and discloses a method for limiting growth and preservation of potato test-tube seedlings. ~4000lx, photoperiod is under the condition of light 16h / darkness 8h, insert in the culture medium and culture respectively for 1 week; (2) low temperature exercise: select the healthy and robust potato plantlets in the selection step (1) and insert them into the special storage bag, The culture temperature and light time are 20°C for 8 hours of light and 4°C for 16 hours of darkness for 1 to 2 weeks of low-temperature exercise; (3) Restricted growth: Select step (2) to grow robust potato sterile test-tube plantlets into a special storage bag, Store at 4°C without light; this storage method is based on tissue culture technology, and the storage period of potato plantlets in test tubes can reach 2 years, forming a low-maintenance, low-consumption, and longer-term technology suitable for medium and long-term storage of potatoes.

Description

technical field [0001] The invention relates to the technical field of in vitro test-tube seedling preservation, in particular to a method for limiting growth and preservation of potato test-tube seedlings. Background technique [0002] Potato (Solanum tuberosum L.) is an annual dicotyledonous herb of the Solanaceae family. Originating in the Andes highlands of countries such as Peru and Bolivia in Latin America, it is the most grown and consumed crop in the world and the fourth largest food crop in the global economy after rice, wheat and corn. In the 16th century, Spanish and British explorers brought potatoes back to their own countries from Latin America, and then spread to Italy and Europe. Potato has a cultivation history of more than 400 years in China, but my country's potato germplasm resources are relatively limited. Potato likes warm and cool, likes light, and is resistant to drought. Due to its short growth cycle, high yield per unit area, rich tuber nutrition,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 张金梅陈晓玲辛霞卢新雄尹广鹍何娟娟
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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