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Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii

A tissue culture and shoot tip technology, applied in the field of plant biology, can solve the problems of low rooting and strong seedling rate and transplanting survival rate, weak rhizome proliferation and bud differentiation, and difficulty in inducing rhizomes, etc., and achieves disinfection effect. Good, high commercial value, short disinfection time effect

Inactive Publication Date: 2010-09-08
ZHEJIANG UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is aimed at how to solve the problem of easy pollution and browning in the primary culture of the stem tip in the tissue culture and rapid propagation of Chunlan famous products, the difficulty and long time of starting and inducing rhizomes, the weak proliferation of rhizomes and the differentiation of buds, and the rooting and strong seedlings rate and low survival rate of transplanting, and propose a method that can solve the above-mentioned technical difficulties, reduce production costs, and realize the large-scale production of high-quality tissue culture seedlings of Chunlan famous brand, that is, a rapid tissue culture method for famous Chunlan stem tip tissue culture. Breeding method

Method used

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  • Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii
  • Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii
  • Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii

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Effect test

Embodiment 1

[0038] The rapid propagation method of the shoot tip tissue culture of Chunlan famous product "Songmei":

[0039] (1) New shoot cutting: at the end of January, the method is to remove the orchid plant from the pot, remove the soil around the new shoot, see the condition of the base of the shoot, and cut it off with a sharp scalpel close to the base of the new shoot, to ensure the integrity of the new shoot as much as possible , so as not to damage the growth point, the new shoots are about 2 cm high (see figure 1 ), and the orchid strains were planted back in the pot after 30 minutes in the air.

[0040] (2) Sprout disinfection and stem tip stripping: Rinse the surface of Songmei sprouts, wipe them with 70% medical alcohol cotton pads, put them in a beaker, add 200ml of tap water, add 2 to 3 drops of Tween-80 in the water, Shake evenly, cover the mouth of the cup with gauze, soak for 30 minutes, rinse with tap water for 2 to 4 hours, soak in distilled water for 5 minutes and ...

Embodiment 2

[0047] The cost-reducing method of replacing sucrose with white sugar in the shoot tip tissue culture rapid propagation of Chunlan famous product "Yuanhong":

[0048] The price of sucrose is about 10 times that of white sugar. If it can be replaced, it can effectively reduce production costs. Cultivate the survey data after 3 months, find out from table 1, table 2, table 3, use white sugar effect to be better than sucrose or similar, the result shows that rhizome proliferation can be selected white sugar 20g / l, and rhizome induces bud and root shape The stem directly induces rooting and strong seedlings, requiring higher carbohydrate nutrition, and 30g / l of white sugar can be used.

[0049] (1) Rhizome proliferation

[0050] The top of the rhizome with a length of 1 cm without bifurcation is selected as the material. From Table 1, the sugar concentration gradient has no significant effect on the proliferation number. The difference between sucrose is 17 bars, and the differen...

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Abstract

The invention provides a method for culturing and rapidly propagating the stem tip tissue of a rare cymbidium goeringii. By taking the stem tip of a bud of the rare cymbidium goeringii as an explants, the method comprises the following steps: inducing rhizome from the stem tip, performing successive multiplication on the rhizome, inducing the bud from the rhizome, rooting and strengthening the seedling, transplanting the test-tube plantlet and the like; an optimal culture medium is chosen at the stages of cutting and sterilizing the bud, stripping the stem tip, inducing the rhizome, multiplying, bud differentiation, rooting and strengthening and the like, so that quick multiplication of the seedling, high yield, good quality and low cost of the rare cymbidium goeringii can be realized to lay the foundation for the industrial production of the cymbidium goeringii. The method of the invention has high operability, well solves the problems of slow multiplication speed, easy degeneration and the like in a traditional division method of the cymbidium goeringii, can gradually realize the industrial production of the cymbidium goeringii species of high commodity value and has high application and popularization value.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and relates to an asexual propagation method through plant tissue culture technology, in particular to a rapid propagation method of stem tip tissue culture of famous and precious varieties of Chunlan. Background technique [0002] Chunlan (Cymbidium goeringii) belongs to the orchid of the genus Orchidaceae. It is a perennial evergreen herb plant. It has fragrant flowers and beautiful leaves. Both flowers and leaves are ornamental and very elegant. It is one of China's top ten famous one of the flowers. Chunlan is mainly distributed in the southeast and southwestern regions of my country. Zhejiang Province is the main producing area of ​​Chunlan. It has a long history of cultivation and has as many as 300 varieties. The plant method has a low reproduction coefficient. Some traditional famous products such as "Lvyun", "Songmei", "Dafugui", "Longzi", "Jiyuan" and so on, although they have been pa...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李方黄焱夏宜平柴明良陈昆松
Owner ZHEJIANG UNIV
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