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Fast tissue culture propagation process for azalea and camellia

A rhododendron red camellia, tissue culture and rapid propagation technology, applied in the field of plant propagation, to meet the needs of large-scale production and protect the mother plant

Inactive Publication Date: 2007-06-27
江苏春辉生态农林股份有限公司
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Problems solved by technology

And the method of tissue culture rapid propagation of Rhododendron red camellia has not been reported yet

Method used

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Embodiment Construction

[0018] The present invention relates to a kind of rhododendron red camellia tissue culture rapid propagation seedling raising method, and it comprises explant material taking and processing, primary culture, subculture, rooting culture process steps, and its specific steps are as follows:

[0019] Step 1. Selection of explants

[0020] On continuous sunny days from April to May, cut the stems of Rhododendron Camellia from the mother plant just after the tender shoots have emerged.

[0021] Step two, explant treatment

[0022] Shake and wash the stem segment selected in step 1 in 1% washing powder water for 6-8 minutes, then rinse it under the tap water for 30 minutes, blot the water with clean gauze, and then work in sterile ultra-clean Soak and disinfect Taichung in 70% alcohol solution for 30 seconds, rinse with sterile water 2 to 3 times, drain the water, and then transfer to 0.1% HgCl 2 Disinfect in the solution for 8 to 10 minutes, rinse with sterile water 5 to 6 times,...

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Abstract

The fast tissue culture propagation process for azalea and camellia includes the following steps: cutting tender stem segment from the maternal plant in April or May; soaking in alcohol solution, sterilizing in HgCl2 solution and cutting the stem tip; inoculating the stem tip in primary culture medium to grow cespitose bud and cutting single bud seedling after the bud grows to 2-3cm length; culturing the single bud seedling in secondary culture medium inside a sealed container to proliferate test tube seedling repeatedly; culturing the test tube seedling in rooting culture medium inside sealed container to grow new root to 0.3-0.5cm long, and transplanting. The process can provide seedling of azalea and camellia continuously.

Description

technical field [0001] The invention relates to a method for rapid propagation of camellia tissue culture. In particular, it relates to a method for tissue culture and rapid propagation of Rhododendron camellia. It belongs to the technical field of plant propagation. Background technique [0002] Rhododendron red camellia is native to China. It was discovered in Ehuangzhang Provincial Nature Reserve, Yangchun County, Guangdong Province, China in 1986. It is a national first-class protected plant. Camellia belongs to the subgroup plant of Red Camellia Group Guangguohong Camellia. [0003] Rhododendron red camellia is a very rare woody flower. It blooms in four seasons. The leaves are smoother than ordinary camellias without sawtooth. Even at a temperature of 38°C, the tree is still full of red flowers, which has high ornamental value. And as a germplasm resource utilization value. Due to its extremely high requirements on the ecological conditions for survival, the natura...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G7/00
Inventor 吴有光刘唤英方建民
Owner 江苏春辉生态农林股份有限公司
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