Chinese toon tissue-culture quick propagation technique and culture medium proportion

Abstract

The invention relates to Chinese toon tissue culture and rapid propagation technology. The technology comprises the following steps: clipping an annual semi-lignified branch with plump axillary buds from a growing plant, clipping the branch into a stem section containing 1 to 2 axillary buds after sterilization, and rapidly inoculating the stem section to a differentiation induction culture medium for differentiation culture; placing the plant obtained through the differentiation culture into a propagation culture medium for propagation and rapid propagation; and when a certain quantity is reached through the propagation, putting the plant into a rootage culture medium for sound seedling rootage culture, and hardening the seedling after the sound seedling takes root. The method carries out the asexual tissue rapid propagation and disinfection through the stem apex and stem section of Chinese toon. The propagated filial generation maintains the good seed nature of the original variety, ensures the seed purity and has no diseases and insects. The technology overcomes the defect of low propagation speed lying in the conventional seed and cut root propagation, enables the artificial control on the culture and growth conditions, is not limited by natural conditions, and has a small amount of drawn material. The technology can realize the industrialized seedling culture and high-efficiency production.

Description

technical field [0001] The invention relates to a tissue culture and rapid propagation technology of plants, specifically a tissue culture and rapid propagation technology of Chinese toon, and the proportion of medium used for breeding. Background technique [0002] Plant tissue culture refers to the process in which all organs, tissues and cells of a plant are placed in a medium containing plant nutrients and regulators under artificial control conditions to allow them to grow and differentiate to form a complete plant. The theoretical basis is the totipotency of plant cells. Plant tissue culture and rapid propagation technology has developed rapidly in recent years, and has been widely used in flowers, fruits, vegetables and crops, and has produced huge economic benefits. [0003] The culture medium is the key to the rapid propagation of plants. The ratio of the culture medium varies with different crops and with different growth and development stages of plants. Differe...

AI Technical Summary

Problems solved by technology

[0004] In the past, the conventional method of producing Chinese toon seedlings was: ① Propagate with root system, but due to too few roots, the amount of seedlings raised is low, and it can only be bred once a year. Slowness; ②The use of seeds to propagate seedlings will cause inconsistencies in the internal and external shapes and growth rates of individual plants, resulting in large differences in quality and nutrient content