Cultivation method for producing hupenine A through in vitro induction proliferation of thallus of huperzia serrata

A technology of Huperzia serrata and Huperzine A, applied in the field of induction and proliferation of isolated Huperzia serrata fronds, can solve the problems of lack of natural Melaleuca pagoda resources, large supply gap of Hup-A, and high cost

Inactive Publication Date: 2012-07-11
JIANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Huperzine A Hup-A drug mainly comes from the whole herb of natural Huperzine serrata. Due to the special curative effect of Huperzine A, scholars at home and abroad have done a lot of work on its total synthesis, derivatives and analogs. Due to the special molecular structure of Hup-A, artificial synthesis is time-consuming, labor-intensive, and expensive. So far, almost no derivatives and analogs with better activity than natural Hup-A have been obtained. Therefore, artificially synthesized Hup-A and its analogs are far from commercialization. There is still a long way to go for production. With the arrival of an aging society, Alzheimer's disease (senile dementia) has become the

Method used

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  • Cultivation method for producing hupenine A through in vitro induction proliferation of thallus of huperzia serrata
  • Cultivation method for producing hupenine A through in vitro induction proliferation of thallus of huperzia serrata
  • Cultivation method for producing hupenine A through in vitro induction proliferation of thallus of huperzia serrata

Examples

Experimental program
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Embodiment 1

[0024] Select robust and pest-free wild Huperus serrata plants, and take new branches about 3cm long that were pumped out in the same year;

[0025] After washing off the dust on the branches and leaves with tap water, soak them in detergent liquid for 6-10 minutes, then brush the branches and leaves with a soft brush, rinse them in tap water, absorb the water droplets with filter paper, and put them into a sterile container for surface cleaning. Sterilization treatment: transfer to the ultra-clean workbench, then sterilize with 75% ethanol for 0.5min--1min, wash with sterile water, blot the water, and then sterilize with 0.1%-0.5% HgCl 2 Add 1 drop of Tween 80 to soak the disinfection material for 6-10 minutes, pour off the liquid medicine, and rinse with sterile water for 6-8 times. Then, under sterile conditions, the new shoots of Huperz serrata were cut into stem segments with a length of about 0.5-1 cm, and inoculated on the prepared thallus differentiation starting med...

Embodiment 2

[0035] Select healthy and pest-free wild Huperzia serrata plants, take the new shoots about 3cm long that were pumped out in the same year, and after cleaning and disinfecting the explants, under aseptic conditions, cut the new shoots of Huperzia serrata into small stems with a length of about 0.5-1cm. Inoculate on the prepared thallus differentiation priming medium and culture. (1) Phalloid differentiation initiation medium: 1 / 2 (6,7-V)+IAA0.5mg / L (units the same below); (2) Phalloid subculture proliferation medium: 6,7-V +NAA0.5, sucrose consumption 20g / L, other are identical with embodiment 1.

Embodiment 3

[0037](1) Phalloid differentiation initiation medium: 1 / 2 (6,7-V)+IAA1.0mg / L (the unit is the same below); (2) Phalloid subculture proliferation medium: 6,7-V +NAA1.0, sucrose consumption 25g / L, other are identical with embodiment 1.

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Abstract

The invention discloses a cultivation method for producing hupenine A through in vitro induction proliferation of thallus of huperzia serrata. The method comprises the following steps: selecting a wilding huperzia serrata plant which is healthy and has no plant disease or insect pests, and taking a new branch which grows in the current year and is 3 cm in length; producing thallus through induction, proliferating into a piece of thalline tissue block with the diameter of 3 cm, and using the two culture mediums as follows: a thalline differentiation launching culture medium and a thalline mulitiplication culture medium; and dividing and subculturing thalline. The cultivation method and a substrate formula provide essential environmental conditions to in vitro induction and proliferation ofthallus of huperzia serrata in production of hupenine A. When the method provided by the invention is used for in vitro culture of huperzia serrata, a great number of medicinal plant resources of thalline of huperzia serrata can be obtained. Significant economic values can be produced when the method is used for enlarging the scale of extracting produced medicine hupenine A (Hup-A) through in vitro culture of thalline.

Description

technical field [0001] The invention relates to a plant biotechnology, that is, a culture method for in vitro induction and proliferation, in particular to a culture method for in vitro induction and proliferation of huperzine A-producing huperzine serrata fronds. Background technique [0002] Huperzia serrata (Thunb.ex Murray) Trev., also known as Melaleuca pagoda, Serratia serrata, Snakefoot grass, etc., is a Huperzia fern in the family Huperzia, and the whole plant is used as medicine. Since it was first reported in China in 1972 that the alkaloid hupenine A (Hup-A) in this plant has the effect of relaxing striated muscles, further research has found that the alkaloid is a potent, reversible and highly selective acetylcholine. Esterase inhibitors. Under low doses of Hup-A, it has a strong inhibitory effect on acetylcholinesterase (Ach E), which significantly increases the content of acetylcholine (Ach) in the synaptic gap in the distribution area, thereby enhancing neuro...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 涂艺声丁明华
Owner JIANGXI NORMAL UNIV
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