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Hormone-autotrophic huperzia serrata in-vitro thallus cloning line for producing huperzine-a

A technology of Huperzia serrata and huperzine A, which is applied in the field of hormone autotrophic Huperzia serrata isolated fronds cloning lines

Inactive Publication Date: 2014-08-13
JIANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in plant tissue culture, it is generally necessary to add exogenous plant growth substances so that the culture can proliferate and grow, and the residue of exogenous plant growth substances in the culture will affect the application.

Method used

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  • Hormone-autotrophic huperzia serrata in-vitro thallus cloning line for producing huperzine-a
  • Hormone-autotrophic huperzia serrata in-vitro thallus cloning line for producing huperzine-a
  • Hormone-autotrophic huperzia serrata in-vitro thallus cloning line for producing huperzine-a

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] . Choose healthy and pest-free wild Huperzia serrata plants, and take new branches about 3 cm long that were drawn in the same year.

[0026] , wash off the dust on the branches and leaves with tap water, then soak them in detergent liquid for 6-10 minutes, then brush the branches and leaves with a soft brush, rinse them with tap water, absorb the water droplets with filter paper, and put them into sterile containers Carry out surface sterilization treatment; transfer to an ultra-clean workbench, then disinfect with 75% ethanol for 0.5-1 min, wash with sterile water, blot the water, and then sterilize with 4.5% H 2 o 2 Disinfect for 5-9 minutes, then use 0.1%-0.5% HgCl 2 Add 1 drop of Tween 80 to soak the disinfection material for 6-10 minutes, pour off the liquid medicine, and rinse with sterile water for 6-8 times. Then, under sterile conditions, cut the new shoots of Hupercia serrata into small stems with a length of about 0.5-1 cm, and inoculate them on the prepar...

Embodiment 2

[0037] Select healthy and pest-free wild Huperzia serrata plants, take new shoots about 3cm long that were pumped out in the same year, and after cleaning and disinfecting the explants, under aseptic conditions, cut the new shoots of Huperzia serrata into small stems with a length of about 0.5-1cm , inoculated on the prepared thallus differentiation initiation medium for culture. (1) Phalloid differentiation initiation medium: 1 / 2(6,7–V) + NAA 0.1-1.0 mg / L (units the same below); (2) Phalloid subculture proliferation medium: 1 / 4MS Or add 20 ~ 30g / L sucrose+NAA0.5 in 1 / 2MS Others are the same as embodiment 1.

Embodiment 3

[0039] (1) Phalloid differentiation initiation medium: 1 / 2(6,7–V) + NAA 0.1-1.0 mg / L, sucrose dosage 20-30g / L; (2) Phalloid subculture proliferation medium: Add 20 ~ 30g / L sucrose+NAA1.0 in 1 / 4 MS or 1 / 2 MS, other is the same as embodiment 1.

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PUM

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Abstract

The invention discloses a hormone-autotrophic huperzia serrata in-vitro thallus cloning line for producing huperzine-a. A method comprises the steps are of (1.1) preparing materials; (1.2) preparing a germfree material from an explant and; (1.3) inoculating the germfree material to a prepared thallus differentiation starting culture medium for culture; (1.4) partitioning a thallus tissue subjected to inducible proliferation into small blocks, transferring the small blocks into a thallus subculture multiplication culture medium for directional culture; (1.5) subculturing the hormone-autotrophic huperzia serrata in-vitro thallus cloning line for producing the huperzine-a. Thallus of the hormone-autotrophic huperzia serrata in-vitro thallus cloning line is directly harvested for preparing tea or serving as an additive of tea, so that a novel health-protection product for resisting aging and enhancing memory can be developed; the medical component, namely huperzine-a, can be extracted from the thallus, so that extremely high economical and social values can be achieved.

Description

technical field [0001] The present invention relates to the plant resource and its biotechnology for creating the medicinal ingredient huperzine A, in particular to a hormone autotrophic huperzine seropods isolated frond cloning line producing huperzine A. Background technique [0002] Huperzia serrata (Thunb.ex Murray) Trev. , also known as Melaleuca Pagoda, Serpentine Lycopodium, Serpentine Grass, etc., is a fern of the genus Hupernia in the family Huperzaceae, and the whole plant is used as medicine. Since it was first reported in China in 1972 that the alkaloid in this plant - hupenine A (Hup-A) has the effect of relaxing striated muscles, further in-depth research has found that the alkaloid is a potent, reversible and highly selective acetylcholine Esterase inhibitors. Under low doses of Hup-A, it has a strong inhibitory effect on acetylcholinesterase (Ach E), which significantly increases the content of acetylcholine (Ach) in the synaptic gap in the distribution are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 涂艺声徐贤柱杨萍蔡险峰陈曼吉枝单
Owner JIANGXI NORMAL UNIV
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