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Method for extracting huperzine A from leaves of huperzia serrata

A technology of Huperzine serpentis and Huperzine A is applied in the field of extracting Huperzine A from Huperzine serpentis leaves, which can solve the problems of time-consuming, low yield, difficulty in large-scale production and the like, and achieve good decolorization effect and decolorization effect. Significant, yield-improving effect

Inactive Publication Date: 2017-01-25
安徽省鸿运生物医药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some people have used solvent extraction, silica gel column chromatography and other methods to separate, although a small amount of products can be obtained, but it is time-consuming (a production cycle is more than one month), the yield is low (less than one millionth), and it is difficult to scale up production, while synthetic products are difficult to obtain ideal curative effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Wash and dry the Huperus serpentine leaves, put them in a fermenter, add a surfactant and fermentation bacteria, and then carry out 24-hour fermentation treatment, wherein the surfactant is a mixture of fatty acid sorbitan and polysorbate, which is mixed with serpentine The specific gravity of fir leaves is the fatty acid sorbitan: polysorbate: huperzia serrata leaves = 2:1:100; then put the fermented huperzia serrata leaves into 75% ether, and at the same time add 10% 2,5- Dihydroxybenzoic acid, after soaking for 2 hours, the solution was centrifuged, and the obtained filter residue was put into 75% ether again, and 10% of 2,5-dihydroxybenzoic acid was added at the same time, and after soaking for 1 hour, it was centrifuged again. Centrifuge the supernatant and mix, add activated carbon to it, after 10 minutes of treatment, remove the activated carbon, heat the solution in a water bath to 70°C until the solution becomes viscous, stop heating, stir the solution evenly, t...

Embodiment 2

[0016] Wash and dry the Huperus serpentine leaves, put them in a fermenter, add a surfactant and fermentation bacteria, and then carry out 24-hour fermentation treatment, wherein the surfactant is a mixture of fatty acid sorbitan and polysorbate, which is mixed with serpentine The specific gravity of fir leaves is fatty acid sorbitan: polysorbate: huperzia serrata leaves = 2:1:150; then put the fermented huperzia serrata leaves into 80% ether, and add 20% 2,5- Dihydroxybenzoic acid, after soaking for 2 hours, the solution was centrifuged, and the obtained filter residue was put into 95% ether again, and 30% of 2,5-dihydroxybenzoic acid was added at the same time, and after soaking for 1 hour, it was centrifuged again. Centrifuge the supernatant and mix, add activated carbon to it, after 15 minutes of treatment, remove the activated carbon, heat the solution in a water bath to 80°C until the solution becomes viscous, stop heating, stir the solution evenly, then cool down to -4 ...

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PUM

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Abstract

The invention discloses an extraction method of huperzine A. The extraction method comprises the following steps: fermentation treatment of leaves of huperzia serrata, diethyl ether soaking, centrifugation, water bath evaporation and cooling crystallization. By virtue of the extraction method, the yield and extraction purity of huperzine A can be greatly increased, and furthermore, the production cost is low; and the extraction method is suitable for industrial production.

Description

[0001] technical background [0002] Huperzine A (Hup A for short), also known as Fordinine, is a natural alkaloid isolated from Huperzine genus Serpentine (melaleuca), which is a powerful and reversible alkaloid. Acetylcholinesterase (AChE) inhibitors have significant curative effects on the treatment of Alzheimer's disease, simple memory impairment and myasthenia gravis, and the drug effect lasts for a long time without serious adverse reactions. It is currently the most effective treatment for the above diseases at home and abroad. Drugs are of great concern. Due to its high therapeutic index and long duration of action, it has been listed as one of the second-generation cholinesterase inhibitors internationally. But Hup A exists in a very low amount in nature and contains a lot of impurities. So far, a large number of researchers at home and abroad are exploring to find an ideal separation method. Some people have used solvent extraction, silica gel column chromatography a...

Claims

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Application Information

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IPC IPC(8): C07D221/22
CPCC07D221/22
Inventor 黄华
Owner 安徽省鸿运生物医药股份有限公司
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