Plant endophytic fungi of huperzia serrata and application thereof in preparing huperzine A

A technology of endophytic fungi and Huperzia serrata, which is applied in the direction of fungi, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of many reaction steps, low yield, harsh reaction conditions, etc., and achieve an increase in yield Large space, rapid growth and reproduction, and the effect of avoiding excessive excavation

Inactive Publication Date: 2014-03-26
ZHEJIANG PHARMA COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Huperzine A has a complex structure, many reaction steps, harsh reaction conditions, low yield, and high environmental pollution. The isomers cannot be separated, resulting in low activity of the compound. The chemical synthesis method cannot be applied to commercial production.

Method used

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  • Plant endophytic fungi of huperzia serrata and application thereof in preparing huperzine A
  • Plant endophytic fungi of huperzia serrata and application thereof in preparing huperzine A
  • Plant endophytic fungi of huperzia serrata and application thereof in preparing huperzine A

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Embodiment Construction

[0027] The present invention will be further described below in conjunction with the embodiments of the accompanying drawings.

[0028] Step 1: Isolation and screening of huperzine-producing Trichoderma sp. L44

[0029] Wild Hupercia serrata plants were collected from Tianmu Mountain, Hangzhou City, Zhejiang Province, and brought back to the laboratory for cultivation.

[0030] Choose fresh and healthy plant Huperzia serrata, wash with cleansing powder, and rinse with running water for 4 hours.

[0031] Separate stems, leaves, and roots on an ultra-clean workbench, sterilize the stems with 0.1% mercuric chloride solution for 15 minutes, soak the leaves and roots in 0.1% mercuric chloride solution for 10 minutes, take out the materials, and rinse them with sterile water 6 times.

[0032]Under sterile conditions, the rhizomes and leaves were cut into 0.5 cm lengths and inoculated on MS medium. The formula of MS medium is: potassium nitrate (KNO3) 1900 mg / L, ammonium nitrate (N...

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Abstract

The invention relates to plant endophytic fungi of huperzia serrata. The biological classification and molecular biological identification are (Trichodermasp.) L44. The bacterium has been already preserved in China Center for Type Culture Collection, with the preserving number of CCTCC NO: M2013198. The invention further provides an application of the plant endophytic fungi of huperzia serrata in preparing huperzine A. The invention provides a novel microorganism for source of the huperzine A. The microorganism provided by the invention has the advantages of quick growth and reproduction, no limitation on external environments such as seasons and climates and large yield promoting space and the like. The wild resource of the medicinal plant huperzia serrata can be effectively prevented from being excessively excavated.

Description

technical field [0001] The invention belongs to the microbial extraction and product cultivation of medicinal plants, in particular to the separation of endophytic fungi from Huperz serrata and the fermentation of endophytic fungi to produce huperzine A. Background technique [0002] A large number of epidemiological survey data show that the aging of the global population has become a world trend. Alzheimer's disease, also known as Alzheimer's disease senile dementia, generally referred to as Alzheimer's disease (Alzheimer's disease, AD), is the most common cause of dementia. The main types have undoubtedly become "common diseases" in modern society. According to studies by Ellis et al., the incidence of dementia in the elderly over 60 years old is 6-12%; as the age grows, the possibility of onset also increases significantly. For example, for the elderly over 85 years old, the incidence of dementia is as high as 20-40%. Although there are many types of dementia, more ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/12C12R1/885
Inventor 董丽辉凌庆枝
Owner ZHEJIANG PHARMA COLLEGE
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