Method for extracting and separating huperzine A and huperzine B from huperzia serrata

A technology of huperzine A and huperzine B, which is applied in the field of alkaloid extraction, can solve problems such as waste of effective resources, and achieve the effect of improving economic benefits

Inactive Publication Date: 2012-05-02
重庆市秀山红星中药材开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current melaleuca extract is mainly concentrated in huperzine A, while huperzine B i...

Method used

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  • Method for extracting and separating huperzine A and huperzine B from huperzia serrata
  • Method for extracting and separating huperzine A and huperzine B from huperzia serrata
  • Method for extracting and separating huperzine A and huperzine B from huperzia serrata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Cleanly select 100kg of melaleuca medicinal materials (raw material content of huperzine A 0.17‰, huperzine B 0.036‰), pulverize, the particle size is 10-20 mesh, and sieve; then the melaleuca powder is washed with 0.8% hydrochloric acid solution Soak in 2200L for 60min, then extract with 600W ultrasonic wave for 15min, then soak for 60min, then centrifuge the extract and take the supernatant; then soak the filter residue in 2000L of 0.8% hydrochloric acid solution for 45min, then extract with 600W ultrasonic wave for 10min , and then soak for 45min, then centrifuge the extract, get the supernatant, and finally combine the two supernatants.

[0023] Add 3 kg of activated carbon to the combined solution, stir and decolorize for 4 hours, filter the activated carbon, and leave the filtrate; absorb the cation exchange resin on the obtained filtrate, and after adsorption for 30 minutes, wash the resin with 4200L of distilled water, then use 4200L of 1% hydrochloric acid to el...

Embodiment 2

[0027] Cleanly select 100kg of melaleuca medicinal materials (raw material content of huperzine A 0.17‰, huperzine B 0.036‰), pulverize, the particle size is 10-20 mesh, and sieve; then the melaleuca powder is washed with 0.8% hydrochloric acid solution Soak in 2500L for 60min, then extract with 600W power ultrasonic wave for 15min, then soak for 60min, then centrifuge the extract, and take the supernatant; then soak the filter residue in 2200L of 0.8% hydrochloric acid solution for 45min, and then extract with 600W power ultrasonic wave for 10min , and then soak for 45min, then centrifuge the extract, get the supernatant, and finally combine the two supernatants.

[0028] Add 3 kg of activated carbon to the combined solution, stir and decolorize for 4 hours, filter the activated carbon, and leave the filtrate; absorb the cation exchange resin on the resulting filtrate, and after adsorption for 30 minutes, wash the resin with 4700L of distilled water, then use 4700L of 1% hydro...

Embodiment 3

[0031] Cleanly select 100kg of melaleuca medicinal materials (raw material content of huperzine A 0.17‰, huperzine B 0.036‰), pulverize, the particle size is 10-20 mesh, and sieve; then use 0.8% hydrochloric acid solution for melaleuca powder Soak in 2000L for 60min, then extract with 600W power ultrasonic wave for 15min, then soak for 60min, then centrifuge the extract, and take the supernatant; then soak the filter residue in 1800L of 0.8% hydrochloric acid solution for 45min, and then extract with 600W power ultrasonic wave for 10min , and then soak for 45min, then centrifuge the extract, get the supernatant, and finally combine the two supernatants.

[0032]Add 3 kg of activated carbon to the combined liquid, stir and decolorize for 4 hours, filter the activated carbon, and leave the filtrate; adsorb the cation exchange resin on the resulting filtrate, and after adsorption for 30 minutes, wash the resin with 3800L of distilled water, then elute with 3800L of 1% hydrochloric...

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Abstract

The invention relates to a method for extracting and separating huperzine A and huperzine B from huperzia serrata, which comprises total huperzine extraction and purification, and huperzine A/huperzine B separation and crystallization. The invention has the following advantages: acid soaking and ultrasonic repeated extraction can sufficiently precipitate total huperzines in the huperzia serrata; activated carbon and cation exchange resin are utilized to remove impurities; reversion phase chromatography columns are utilized to separate huperzine A and huperzine B; and concentration crystallization and recrystallization are respectively utilized to enhance the purity of the huperzine A and huperzine B. The invention effectively utilizes natural resources, enhances the recovery rate and extract content, and improves the economic benefit.

Description

technical field [0001] The invention relates to a method for extracting alkaloids, in particular to a method for extracting and separating huperzine A and huperzine B from a Melaleuca tower. Background technique [0002] The Melaleuca Pagoda is a fern of the genus Huperzia in the family Huperzaceae, also known as Huperzia serrata and King of Help. It is mainly distributed in the Northeast, the Yangtze River Basin and Fujian, Guangdong, Guangxi, Yunnan, Guizhou and other provinces. Folks often use the whole herb as medicine, which is poisonous and used for reducing fever, hemostasis, reducing swelling and detoxification, and traumatic injuries. The alkaloids contained in Melaleuca, especially huperzine A (HupA) and huperzine B (HupB) are acetylcholinesterase inhibitors with the advantages of low toxicity, high efficiency, reversibility and high selectivity. Myasthenia, middle-aged and elderly dementia, and improving memory have good curative effects, and have a certain effe...

Claims

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Application Information

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IPC IPC(8): C07D221/22C07D471/08
Inventor 赵勇彪祝利杨再江向廷军何全慧
Owner 重庆市秀山红星中药材开发有限公司
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