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Effective huperzia serrata callus culture method

A Huperzia serrata and callus technology, applied in the agricultural field, can solve the problems of incapable of large-scale mining, limited growth rate, long resource renewal cycle, etc., and achieve the effect of solving the problem of endophytic fungus pollution

Inactive Publication Date: 2017-05-10
JIANGXI AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, due to the scattered resources of Huperzia serrata, and many relatively concentrated production areas are located in nature reserves and scenic areas, and Huperzia serrata is a perennial, slow-growing small herb, the annual net biomass growth rate is extremely limited , to grow into a seedling with a height of 12cm, it takes about 6 to 7 years under natural conditions, the resource renewal cycle is long, and large-scale exploitation is not possible. In the prior art, there is an urgent need for an effective Huperzia serrata callus culture method

Method used

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Embodiment Construction

[0014] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0015] 1.1 Materials

[0016] The experimental materials were collected from Lushan Mountain, Jiangxi Province, and introduced into the greenhouse of Jiangxi Agricultural University by Teacher Hu Songping in December 2014.

[0017] 1.2 Medium

[0018] 1.2.1 Culture medium type

[0019] Four media: (1) MS+0.1μM IBA+1.5μM KT+3mg / L glutamine; (2) 1 / 2MS+0.1μM IBA+1.5μM KT+3mg / L glutamine; (3) 1 / 2MS+3mg / L glutamine / hormone-free; (4)MS+3mg / L glutamine / hormone-free

[0020] 2.2.2 Medium sterilization

[0021] Autoclave, sterilization time is 15min, sterilization temperature is 121℃

[0022] 1.3 Method

[0023] 1.3.1 Selection and disinfection of explants

[0024] Two disinfection methods:

[0025] (1). Clean with detergent 2 times → wash 4 times with sterile water → sterilize with 0.15% mercury solution for 5’→ rinse with sterile water...

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Abstract

The invention discloses an effective huperzia serrata callus culture method. The method includes: adopting a detergent to wash a huperzia serrata stem tip twice, adopting sterile water to wash the same for 4 times, adopting 0.15% mercuric chloride liquid to sterilize for 5min, and adopting sterile water to wash for 4-6 times; taking down a micro stem tip of 0.1-0.2mm in length from the huperzia serrata stem tip under an anatomical lens, adopting an MS culture medium, adding 0.1uM of IBA, 1.5uM of KT and 3mg / L glutamine, and performing photoperiod culture at constant temperature of 25 DEG C for 14h. A method of repeated sterilization combined with AAS to inhibit endophyte is adopted, so that the problem of endophytic fungi pollution in the process of huperzia serrata callus culture is solved, and callus induction rate is higher than 25% generally.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to an effective method for culturing Huperzia serrata callus. Background technique [0002] Huperzia serrata (Thunb) Trev. is a fern that belongs to Huperiaceae and Huperzia Bernh. Folks are used to treat carbuncle, furuncle, swollen poison, bruises, etc. Distributed all over the country, also widely distributed in other parts of Asia, Oceania, Cuba, Mexico and other places in Central America [1] . It mostly grows in the dense forests of mountains or in the damp soil of valleys, with an altitude of 400m to 1600m. The upper tree species of the community mainly include Phyllostachys heterocyc la and Cephalotaxus fortunei, and the shrub species include Loropetalum chinense, Lonicera japonica, Rhododendronmariesii, Maesa japonica, Eurya muricata, herbaceous plants include fern, Polygonatum cyrtonema, Aster geratoides, Eupatorium ( Eupatorium japonicum) and Briggsia c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 胡颂平张琳郑敏毛伟伟杜鹃余建
Owner JIANGXI AGRICULTURAL UNIVERSITY
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