Method for producing A by using huperzia serrata prothallium

A technology of huperzine A and Huperzia serrata, which is applied in the field of plant biology, can solve the problems of small reserves and low content of Huperzia serrata, and limit the regeneration of wild resources of Huperzia serrata, and achieve large-scale production and content differences. small effect

Inactive Publication Date: 2012-07-18
NORTHWEST UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of huperzine A in Huperzia serrata is very low, only about 0.007%, and most of the existing plants of this type grow slowly. After germination, it belongs to the subterranean gametophyte, which takes 6-15 y

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Induction of prothallus

[0023] Soak the sporangia of Hupermus serrata in a clean bench first in 70% ethanol solution for 30s; then place them in 0.1% HgCl 2 Soak in the solution for 8 minutes, then rinse with sterile water for 3 times, and use the sporangia or the spores released from the sporangia after the sporangia rupture as explants induced by prothallus; inoculate the obtained explants in conventional MS medium , cultured for 12 weeks under conditions of a culture temperature of 22±2°C, a light time of 12h / d, and a light intensity of 1000Lx, and green prothallus tissues grew from the explants;

[0024] (2) Culture of prothallus

[0025] Cut the obtained prothallus tissue into 0.3 cm 2 Then transferred to the production medium containing 2mg / L iron trichloride huperzine A to continue culturing, the culture conditions were the same as in step (1), that is, the culture temperature was 22±2°C, the light time was 12h / d, and the light The strength is 1000Lx, an...

Embodiment 2

[0029] (1) Induction of prothallus

[0030] Soak the sporangia of Hupermus serrata in a clean bench first in 70% ethanol solution for 30s; then place them in 0.1% HgCl 2 Soak in the solution for 8 minutes, then rinse with sterile water for 3 times, and use the sporangia or the spores released from the sporangia after the sporangia rupture as explants induced by prothallus; inoculate the obtained explants in conventional MS medium , cultured for 12 weeks under conditions of a culture temperature of 22±2°C, a light time of 12 / d, and a light intensity of 1000Lx, and green prothallus tissues grew from the explants;

[0031] (2) Culture of prothallus

[0032] Cut the obtained prothallus tissue into 0.3 cm 2 Then transfer to the production medium containing 10mg / L iron trichloride huperzine A to continue culturing. The culture conditions are the same as in step (1), that is, the culture temperature is 22±2°C, the light time is 12h / d, and the light time is 12h / d. The strength is 1...

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Abstract

The invention discloses a method for producing huperzine A by using huperzia serrata prothallium. The huperzine A is produced from in vitro huperzia serrata prothallium by mass culture. The method is simple and easy to operate, provides new ways for sources of the huperzine A, and protects resource of wild huperzia serrata simultaneously.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for producing huperzine A by utilizing the prothallus of Huperz serrata serrata. Background technique [0002] Huperzia serrata (Thunb.) Trev., also known as Melaleuca pagoda, Sanzhi and snake foot grass, etc., is a perennial herbaceous fern of the Huperoaceae Huperoaceae Huperzia Bernh. The whole herb is mainly used in Chinese folk. It is used to treat diseases such as bruises, congestion, swelling and pain, and schizophrenia. Since my country's scientific and technological workers first reported that huperzine A isolated from this plant has a striated muscle relaxation effect in 1972, a large number of studies have been carried out on it, and it has been found that huperzine A is a highly efficient, low-toxic, reversible and highly selective It is a potent acetylcholinesterase inhibitor and has obvious curative effect on the treatment of Alzheimer's disea...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 郭斌尉亚辉包日双徐玲玲
Owner NORTHWEST UNIV
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