Application of endogenous fungus in cultivation of snow lotus seedling and snow lotus

A technology of endophytic fungi and symbiotic culture, applied in the field of bioengineering, can solve problems such as the shortage of medicinal resources

Inactive Publication Date: 2007-05-16
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Another object of the present invention is to promote the growth and development of snow lotus seedlings, improve the disease resistance of cultivated snow lotus, increase the co

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. The cultivation of DSE-1 fungus (Mycocentrospora sp.)

[0028]After the DSE-1 fungus was activated from the slant test tube strains preserved at low temperature, it was transferred to a PDA plate and incubated at a constant temperature of 25°C for 15 days. A φ5mm hole punch was used to punch holes on the edge of the colony in the plate to form bacterial flakes. The fragments were cultured in a liquid culture medium.

[0029] The medium composition of the DSE-1 liquid culture is: 2% of glucose, 0.3% of potassium dihydrogen phosphate, 0.1% of magnesium sulfate, 4% of natural wheat bran (boiled juice), and the above components are calculated by weight percentage. The pH of the medium is 5.6, 250mL of medium in a 500mL Erlenmeyer bottle, sterilized at 121°C for 30 minutes, and the above-mentioned DSE-1 (Mycocentrospora sp.) bacterial slices are inserted. The culture condition is that the shaking speed of the shaker is 120 rpm, and after 23 days of dark culture at 23°C, ...

Embodiment 2

[0038] 1. Cultivation of DSE-2 fungus (Leptodontidium sp.)

[0039] After the DSE-2 fungus was activated from the slant test tube strain preserved at low temperature, it was transferred to a PDA plate, and cultured at a constant temperature of 25°C for 20 days, and a φ5mm hole punch was used to punch holes on the edge of the colony in the plate to form a bacterial sheet. The fragments were cultured in a liquid culture medium.

[0040] The medium composition of the DSE-2 fungus liquid culture is: 3% of glucose, 0.2% of dipotassium hydrogen phosphate, 0.25% of magnesium sulfate, 2% of natural wheat bran (boiled juice), and the above components are calculated by weight percentage. The pH of the medium is 5.8, 200mL of the medium in a 500mL Erlenmeyer bottle, sterilized at 121°C for 30 minutes, and the above-mentioned DSE-2 (Leptodontidium sp.) bacterial slices are inserted. The culture conditions are shaker shaking speed 100 rev / min, 25° C. dark culture for 15 days, and it is th...

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PUM

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Abstract

The invention relates to a method for using endogenous fungus to cultivate saussurea sp, wherein two used endogenous fungus are DSE-1(Mycocentrospora sp.) and DSE-2(Leptodontidium sp.); and the method comprises that cultivating fungus and saussurea seed, then transplanting them into symbiotic substrate to be cultivated. The invention can avoid seed maintain process or shorten said process. And it can avoid hormone and chemical accelerator, to improve the quality and the content of active component.

Description

technical field [0001] The invention belongs to the technical field of bioengineering. Specifically, it relates to the application of two kinds of endophytic fungi in the cultivation of snow lotus seedlings and the cultivation of snow lotus and the growth-promoting effect on the snow lotus. Background technique [0002] Saussurea DC. is a plant of the genus Saussurea DC. in the family Asteraceae. It is a kind of rare and precious Chinese grass commonly used by the people in the alpine regions of my country. It is distributed in the alpine regions of Xinjiang, Qinghai, Gansu, Sichuan, Yunnan and Tibet. It is widely used by the people. It is used in the treatment of rheumatoid arthritis, cold pain in women's lower abdomen, amenorrhea, retained placenta, impermeable measles, lung-cold cough, impotence and other diseases. In recent years, the basic research and application development of snow lotus has been deepened, and it has developed into a new anti-inflammatory, analgesic a...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/00A01C1/00C12N1/14A01H4/00A01H3/00
Inventor 郭顺星武励勤陈晓梅王春兰孟志霞肖培根
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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