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Muscodor endophytic fungi ZJLQ070 and application thereof and fungicide

A technology of plant endophytic fungi and fungicides, applied in the direction of plant growth regulators, fungicides, botanical equipment and methods, etc., can solve problems such as irrational drug use, high residues, and high pesticide residues

Active Publication Date: 2010-04-07
ZHEJIANG DAYANG BIOTECH GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemically synthesized agents has brought a series of serious consequences: 1) High residues: due to the carcinogenicity and toxicity of chemically synthesized agents, including various raw materials, it is not easy to get rid of them when entering animals and plants, and the environmental degradation ability is poor, resulting in pesticide residues High; 2) Destruction of ecological balance: long-term evolution and adaptation of organisms, the relative number of various organisms in nature has reached a dynamic balance, and the use of chemical agents has greatly reduced the number of natural enemies of harmful organisms; 3) The emergence of drug resistance: pathogenic bacteria and insects in nature The gene of the class has a strong ability to mutate, and irrational drug use and blind drug use promote the rapid emergence of drug resistance

Method used

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  • Muscodor endophytic fungi ZJLQ070 and application thereof and fungicide
  • Muscodor endophytic fungi ZJLQ070 and application thereof and fungicide
  • Muscodor endophytic fungi ZJLQ070 and application thereof and fungicide

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1, separation and purification of endophytic fungal strains

[0037] (1) Collection of plant tissue specimens: from Fengyang Mountain National Nature Reserve, Zhejiang, China (Fengyang Mountain Nature Reserve is located in Longquan City, southwest of Zhejiang Province, with a geographical location of 119°06′~119°15′E, 27 °46′~27°58′N, the climate is a typical mid-subtropical marine monsoon climate), plant specimens were collected at an altitude of 1200~1500m, including Chinese goosebeery and Camptotheca acuminata, and the collection parts included roots, Stems, leaves and fruits should be stored in fresh-keeping bags after collection, and the storage time at 4°C should not exceed 48 hours.

[0038] (2) Isolation and cultivation of endophytic fungi: the plant specimen tissue of step (1) is rinsed with tap water for the ash layer and attachments on the surface, dried the surface moisture, and sterilized by 75% absolute ethanol and 0.5% sodium hypochlorite. Roo...

Embodiment 2

[0039] Embodiment 2, endophytic fungus screening of producing volatile mixture VOCs

[0040] Adopt grid culture dish confrontation culture method, pour the melted PDA culture medium into grid plastic culture dish (90mm), cut the endophytic fungus bacterium cake with the stainless steel puncher of diameter 5mm, insert culture dish side, 25 After culturing at ℃ for 2d-5d, insert the pathogenic indicator bacteria on the other side of the PDA plate, and at the same time take only the pathogenic indicator bacteria as a control, and seal it with a parafilm sealing film. The growth and antibacterial conditions of the fungi on the colony of the indicator pathogenic bacteria were measured, and the diameter of the colony of the indicator bacteria was measured, and the endophytic fungi that could produce volatile mixture VOCs and inhibit the growth of plant pathogenic bacteria were screened out.

[0041] The 158 isolated and purified endophytic fungal strains were screened for their biol...

Embodiment 3

[0042] Embodiment 3, the test of Muscodor sp. bacterial strain ZJLQ070 antibacterial activity spectrum

[0043] The endophytic fungi Muscodor sp. strain ZJLQ070 and Muscodor albus (as disclosed in patent 200480025237.X) were grown and activated on PDA medium for 10-15 days for later use (the culture temperature was 25° C.). Botrytis cinerea, Rhizoctonia solani, Alternaria tengula, neat small sclerotium, Pythium ultima, Fusarium oxysporum cucumber-specialized type, Neptonia rotundum, Verticillium dahliae, Sclerotinia sclerotiorum , Polychaete persimmona, Pyricularia oryzae, Penicillium digitatum, Botrytis faba, Paecilomyces spp., Aspergillus, Paecilomyces lilacinus were activated on PDA medium, and transferred to PDA fresh On the culture medium, use it after growing for 3-7 days at 25°C; Streak Saccharomyces cerevisiae on the YEPD solid medium, pick a single colony, inoculate in the YEPD liquid culture medium and shake the bacteria (200r / min, 30°C), after 48h Use: Streak Esche...

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Abstract

The invention discloses endophytic fungi Muscodor sp. The preservation name of the strain is ZJLQ070, the preservation unit is China General Microbiological Culture Collection Center, the preservation date is January 8, 2009 and the preservation number is CGMCC 2864. The endophytic fungi can be prepared into living preparations and can be used for suppressing or killing pathogenic microorganisms or musty microorganisms. The invention simultaneously discloses a fungicide comprising 2-methyl-methyl propionate, 2-methylpropionate and the like, especially comprising alpha-phellandrene, beta-phellandrene, trans1-methyl-4-(1-methyl ethyl)-2-cyclohexene-1-alcohol, cis1- methyl-4-(1-methyl ethyl)-2-cyclohexene-1-alcohol, trans3-methyl-6-(1-methyl ethyl)-2-cyclohexene-1-alcohol and cis3-methyl-6-(1-methyl ethyl)-2-cyclohexene-1-alcohol.

Description

technical field [0001] The present invention relates to the fields of microbiology and fungicides, in particular, the present invention relates to a new Muscodor plant endophytic fungus and volatile mixtures based on or derived from it and their uses. Background technique [0002] Fungi are a huge family of microorganisms. Fungi are closely related to humans. Fungi can be used to manufacture important products in industry and agriculture such as medicine, food, and chemical industry. The famous penicillins and cephalosporins are one of the most precious drugs brought to the medical world by fungi so far, and they are also the most important antibiotics at present. They are widely used in the treatment of bacterial infections and have saved thousands of human lives. life. [0003] Plant endophytic fungi (endophytic fungi) is an important group of fungi, according to Dreyfuss and Chapel estimated that the total number of endophytic fungi is 1 million species. A few species h...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P1/00A01P3/00A01N37/02A01N31/06A01N27/00C12R1/645
Inventor 章初龙王国平林福呈毛黎娟周转忠
Owner ZHEJIANG DAYANG BIOTECH GROUP
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