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Endophytic fungi for improving salviae miltiorrhizae yield and content of effective components and application thereof

An endophytic fungus and Salvia miltiorrhiza technology is applied in the field of microorganisms to achieve the effects of promoting biosynthesis, increasing the area of ​​a single leaf, and increasing biomass

Active Publication Date: 2017-06-06
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report on the isolation of endophytic fungi that can simultaneously increase the components of tanshinone and salvianolic acids from Danshen, and there is no report of Chaetomium globosa that can increase the biomass and active ingredient content of Danshen plants

Method used

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  • Endophytic fungi for improving salviae miltiorrhizae yield and content of effective components and application thereof
  • Endophytic fungi for improving salviae miltiorrhizae yield and content of effective components and application thereof
  • Endophytic fungi for improving salviae miltiorrhizae yield and content of effective components and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Isolation and purification of endophytic fungi from Salvia miltiorrhiza

[0056] The endophytic fungus of Salvia miltiorrhiza of the present invention is a strain obtained from leaves of Danshen root in Ruicheng, Shanxi.

[0057] The endophytic fungus of Salvia miltiorrhiza of the present invention is separated and obtained according to the following steps: washing with tap water for 30 minutes to remove sediment, and then washing with deionized water for 3 times. Surface disinfection of leaves was carried out according to the following procedure: 75% ethanol, 30s→1.3M sodium hypochlorite (3-5% available chlorine), 1min→75% ethanol, 30s→sterile deionized water washing 3 times. After the filter paper was blotted to dry the residual moisture, the leaves were cut into 1cm×1cm tissue pieces with a sterilized scalpel, and placed in PDA containing 100mg / L penicillin (potato 200g / L, glucose 20g / L, agar, 15g / L) culture medium at 28°C. The samples were observed for...

Embodiment 2

[0060] Embodiment 2: the cultivation of the aseptic seedling of Salvia miltiorrhiza

[0061] The salvia miltiorrhiza seed of the present invention is purchased from Shanxi Shangluo Tianshili Danshen planting base.

[0062] The aseptic seedlings of Salvia miltiorrhiza of the present invention are cultivated according to the following steps: soak the seeds of Salvia miltiorrhiza in hot water at 40-50°C for 2 hours, wipe off the sticky matter on the surface of the seeds with sterile gauze; then carry out disinfection treatment by conventional disinfection method: 75% Ethanol 30s, 2% NaClO 3 min, 75% ethanol for 30 seconds, rinsed with sterile water for 10 times, and finally inoculated on MS medium at 25°C, 16h light / 8h dark culture, and transferred to new MS medium after seed germination to obtain sterile seedlings.

Embodiment 3

[0063] Example 3: Determination of the content of active components of tanshinones and salvianolic acids in the roots of Salvia miltiorrhiza

[0064] When preparing the seed root of the Salvia miltiorrhiza plant and harvesting, the soil on the root of the Salvia miltiorrhiza root was washed off, washed with distilled water for 3 times, and the residual water was blotted with filter paper. After drying at 50°C to constant weight, determine the content of active ingredients of tanshinone and salvianolic acid in the ingredients according to the following method.

[0065] High performance liquid chromatography (HPLC) was used to analyze the content of active ingredients of tanshinone and salvianolic acid, mainly to quantitatively detect the content of rosmarinic acid, salvianolic acid B, tanshinone I, tanshinone IIA, cryptotanshinone and dihydrotanshinone I . Specific steps are as follows:

[0066] Preparation of samples for HPLC injection of Salvia miltiorrhiza root: Precisely ...

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Abstract

The invention relates to the technical field of microorganisms, and in particular, relates to salviae miltiorrhizae endophytic fungi which are separated from labiatae salvia plant salviae miltiorrhizae plant living bodies by an endophytic fungi separation and purification technology, are classified and named as chaetomium globosum D68 and have the preservation number of CGMCC No.12622. The invention further discloses an application of the salviae miltiorrhizae endophytic fungi in improvement of the host salvia miltiorrhiza biomass and the content of tanshinones and phenolic acids. The salvia endophytic fungi can effectively improve the salvia miltiorrhiza root biomass through co-culture with salvia miltiorrhiza plants, significantly increase the plant crown breadth, height and single-leaf area, also can effectively promote biological synthesis of tanshinones, phenolic acids and other effective components in salvia miltiorrhiza, and have great application value.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to an endophytic fungus for increasing the yield of salvia miltiorrhiza and the content of active ingredients and its application. Background technique [0002] Salvia miltiorrhiza Bge. is a plant of the genus Salvia in the Labiatae family. Its dried roots and rhizomes are used as medicine with the same name. First recorded in "Shen Nong's Materia Medica", it is listed as the top grade. It is a traditional Chinese medicine commonly used in my country. It tastes bitter and is slightly cold in nature. Salvia miltiorrhiza is one of the most widely used and most important traditional Chinese medicine varieties in China. The active components of Danshen are mainly divided into two categories: water-soluble components - phenolic acid compounds, including protocatechualdehyde, danshensu, caffeic acid, rosmarinic acid, salvianolic acid, etc.; fat-soluble components - tanshinones Compound...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 郑承剑秦路平翟欣李秀清韩婷张巧艳蒋益萍贾敏
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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