Breeding method for performing physical mutagenesis on coreopsis tinctoria

A technology of chicken chrysanthemum and physics, which is applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of small crown width, reduced medicinal value, small leaf area, etc., and achieves improved germination rate and easy operation. , well-structured effect

Inactive Publication Date: 2019-03-22
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, no research related to breeding of Coreopsis bicolor, especially mutation breeding has been found so far
[0004] From a breeding point of view, the breeding of Coreopsis bicolor is more suitable for the environment in the plains, because the natural environment in the plains is not as harsh as on the plateau, but there are air and soil pollution in the plains, which may

Method used

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  • Breeding method for performing physical mutagenesis on coreopsis tinctoria
  • Breeding method for performing physical mutagenesis on coreopsis tinctoria
  • Breeding method for performing physical mutagenesis on coreopsis tinctoria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] 1 Materials and methods

[0038] 1.1 Materials

[0039] The test material was the seeds of ‘Kunlun Two-color Coreopsis’ from Kunlun Mountains (purchased from Luoyang Gengyuan Agricultural Development Co., Ltd.).

[0040] 2 methods

[0041] Exploration of the imbibition time of seed germination: firstly, through previous experiments, it was known that the seeds were first imbibed in distilled water and then irradiated with ARTP, and the treatment effect was better. Therefore, before using the atmospheric pressure room temperature plasma breeding instrument to irradiate the two-color coreopsis seeds with distilled water for 0h, 6h, 12h, 18h, 24h, 36h and 48h imbibition and water absorption treatment, the number of seeds for each treatment is 20 Then take pictures and count the germination rate on the 4th day after the imbibition treatment, to determine the best imbibition time for seed germination. As a result, it was found that the germination rate was the highest in ...

Embodiment 2

[0067] Carried out in the same manner as in Example 1, the difference is that 50 seeds were used each time, irradiated for 22.50 minutes, and the experiment was repeated 5 times independently, and the same method was used to measure germination potential, germination rate, leaf area, flower diameter and T t Mutagenesis rate, the results are shown in Table 5 below. As can be seen from the results of Table 5, in five repeated tests, the germination rate can be stabilized at more than 80%, and the leaf area is at 170mm 2 Above, the crown width is above 45mm, T t Mutation rate is not less than 8%.

[0068] Table 5. Effect of ARTP on germination rate, leaf area, crown width and T after 22.50 minutes of irradiation t The effect of mutagenesis

[0069] frequency

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Abstract

The invention relates to a breeding method for performing physical mutagenesis on coreopsis tinctoria. The method is characterized by comprising the following steps: (1) imbibing coreopsis tinctoria seeds in water so as to obtain imbibed seeds; (2) performing plasma irradiation on the imbibed seeds so as to obtain irradiated seeds; (3) germinating and/or growing the irradiated seeds; (4) selectinga strain meeting the breeding objective according to characters shown by germination and/or growth of the seeds. The method disclosed by the invention has the advantages that diversified coreopsis tinctoria mutation types can be obtained; the operation is simple and convenient; the breeding method does not cause environmental pollution and is safe to the operator; the germination rate and/or germination potential of the coreopsis tinctoria seeds can be improved; growth of the leaves can be promoted, the crown breadth of plants is improved, and the quantity of the plant leaves is increased, sothat the leaves grow and/or widen, the leaf area is enlarged, the leaf stalks are lengthened, and the flower shape and/or flower color and the like can be changed.

Description

technical field [0001] The invention belongs to the field of plant mutation breeding, and in particular relates to a method for physical mutation breeding of Coreopsis bicolor. Background technique [0002] Two-color Coreopsis (Coreopsis tinctoria Nutt.) is Compositae (Comppositae) Coreopsis (Coreopsis L.) (Institute of Botany, Chinese Academy of Sciences. "Flora of China" Volume 75 [M]. Beijing: Science Press, 1979 :364.), also known as 'Kunlun snow chrysanthemum', is an annual herb that grows naturally in the alpine mountainous area above 3 200m above sea level in the north of the Karakorum Mountains. Rich in bioactive components such as flavonoids and polyphenols, it is a natural plant integrating health care, health care and medicinal materials. Coreopsis bicolor has the effects of clearing away heat and detoxification, promoting blood circulation and removing blood stasis, harmonizing the stomach and strengthening the spleen (Hu Zhengmei, Ma Qinghe. Kunlun Research pro...

Claims

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Application Information

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IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 赵喜亭许言吕萌宋灵雨马孟丹马明倩李明军
Owner HENAN NORMAL UNIV
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