Method for preparing CYCLIC DIPEPTIDES compounds and use thereof

A technology of compound and cyclic dipeptide, which is applied in the field of marine microbiology, can solve the problems of unreachable and excessive pesticide residues, and achieve the effect of low production cost, no pollution, simple and feasible method

Inactive Publication Date: 2008-12-31
SHANDONG UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main reason why some products in our country are not high-quality and pollution-free is that the pesticide residues exceed the standard.

Method used

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  • Method for preparing CYCLIC DIPEPTIDES compounds and use thereof
  • Method for preparing CYCLIC DIPEPTIDES compounds and use thereof
  • Method for preparing CYCLIC DIPEPTIDES compounds and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Fermentation of the fungus Penicillium commune and preparation of rings (L-leu-L-isoleuci)

[0025] 1) Put the NHS35-04 strain on a solid medium: 150ml-200ml of potato juice, 1g-2g of yeast powder, 10g-15g of glucose, 15g-20g of NaCl, MgCl 2 ·6H 2 O1.1g-1.3g, KCl0.1g-0.2g, FePO 4 0.005g-0.01g, water 1000mL, agar 15g-17g, pH value 6-7, culture at 25°C for 5-7 days; then insert culture medium, potato juice 150ml-200ml, yeast powder 1g-2g, glucose 15g- 20g, NaCl10g-15g, MgCl 2 6H 2 O1.1g-1.3g, KCl 0.1g-0.2g, FePO 4 0.005g-0.01g, 1000mL of water, pH 6-7, 25°C loading volume 100mL / 250mL and culture for 12 days.

[0026] 2) Filtrate the fermentation broth with 30L of gauze to obtain mycelium and water layer, dry the mycelium and put it in a volumetric flask, extract it ultrasonically with acetone until there is no residue after the solvent is recovered under reduced pressure, and the dry extract is dissolved repeatedly in methanol , to obtain 10 g of methanol...

Embodiment 2

[0027] Embodiment 2: Cyclic (L bright-L-different bright) compound promotes the activity test of wheat root and bud growth

[0028] Soak dry and mature wheat seeds in the aqueous solution of ring (L-bright-L-isobright) and incubate in the dark at 25°C. After 48 hours, take out the wheat seeds, measure the length of the shoots and roots of the seeds, and compare them with the blank group to calculate the shoots and roots Growth rate, ring (L-bright-L-different bright) all showed the activity that promotes root system growth of wheat under almost all tested concentrations, up to about 30%, and the effect to bud is very weak or has no effect (see table 1).

[0029] Table 1 Effects of rings (L-bright-L-isobright) on the growth of wheat roots and shoots

[0030]

Embodiment 3

[0031] Embodiment 3: Cyclic (L-bright-L-different bright) compound is to the growth-promoting effect of cucumber

[0032]The seeds of the tested cucumber (Cucumis sativus L.) are "Lu 3" disease-resistant variety, ring (L-liang-L-isobright), with a content of 90%. Use distilled water to make 1.0, 3.0, 5.0, 8.0, 10.0mg / L solutions respectively for later use. For the determination of growth indicators, the root length refers to the distance between the lowest point of the root of the plant and the soil surface, and the plant height refers to the distance from the soil surface to the highest growth point. The length between them, the stem thickness refers to the thickness at 2cm below the cotyledon, and the dry weight of the underground part (including root system and underground stem) and aboveground part of the seedling is measured by the drying method at 80°C;

[0033] (1) Effect of ring (L-bright-L-isobright) treatment on germination of cucumber seeds

[0034] Soak cucumber...

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Abstract

The invention provides a method for preparing a cyclic dipeptides compound as well as an application thereof, the method is to obtain the cyclic dipeptides compound by fermenting, separating and purifying separated endophytic fungi with the Collection No. of CGMCC2094, the compound can effectively induce the system resistance of crop and obviously promote the growth of roots, stems and leaves of plants at different seedling stages, and can enhance the quality of seedlings and promote the formation of strong seedlings, and the method has advantages of convenient and feasible process, non environmental pollution, short period, low cost of materials, easy formation of large-scale batch production and low production cost.

Description

technical field [0001] The present invention belongs to the field of marine microbes, and specifically relates to the fermentation, cultivation and purification of endophytic fungi isolated from the intertidal zone mangrove plant Neptonia nigra, and also relates to the application of the compound as promoting plant growth and inducing plant disease resistance. The strain is preserved in the General Microbiology Center of China Committee for the Preservation of Microbial Strains, the address is No. 13, Zhongguancun Yiyi, Haidian District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, the preservation date is June 18, 2007, the preservation number is CGMCC2094, and the classification name is Penicillium penicillium commune. Background technique [0002] Cyclic dipeptides are found in humans, animals, plants, fungi, and bacteria. Cyclic dipeptides with different structures have different biological functions. The functions discovered so far include inhibiting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02A01N43/60A01P21/00C12R1/80
Inventor 陈靠山田黎裴月湖华会明张久明
Owner SHANDONG UNIV
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