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Method for screening anti-fusarium graminearum toxin of wheat crops

A technology of rubella and crops, applied in the field of crop genetics and breeding, can solve problems affecting human health, lack of control technology, and low efficiency of high-resistant materials

Active Publication Date: 2011-06-29
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wheat head blight can not only lead to a serious decline in yield, but more seriously, due to the accumulation of toxins such as deoxynivalenol produced by pathogenic bacteria in diseased wheat kernels, it affects human health
Due to the lack of effective control technology and the environmental pollution caused by chemical control, breeding resistant varieties is the most effective way to prevent wheat head blight
The practice of wheat head blight resistance breeding at home and abroad for many years shows that the efficiency of traditional hybrid breeding technology for breeding high-resistant materials is low, and a large number of hybrid progeny groups are needed to support

Method used

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  • Method for screening anti-fusarium graminearum toxin of wheat crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0145] Example 1: Gibberella virus-resistant treatment of barley anthers and microspore extraction

[0146] Select middle part floret microspore development to be in the tassel of uninucleate early stage, middle stage from Daejeon, put into refrigerator and refrigerate for 15 days. When inoculating, the tassels are sterilized with saturated bleach solution for 15 minutes, and rinsed with sterile water 3-4 times. 40 anthers were placed in each petri dish, with 1.5×10 -4 After the 0.3M mannitol extract of mol / L rubella toxin is pretreated in the dark for 3 days, pour 15ml of 0.3M mannitol extract into it, and use a high-speed disperser of 3000rpm for ultra-speed rotary cutting, filter with a 150-mesh sieve, and filter the filtrate with 800rpm low-speed centrifugation, repeated 3 times, to collect microspores.

Embodiment 2

[0147] Embodiment 2: the acquisition of gibberellin virus-resistant embryoid body

[0148] Induction medium with N 6 The culture medium is basic medium, which is added with gibberellum crude toxin 0.75×10 -4 mol / L, 2,4,5-T 0.5mg / L, KT 0.5mg / L, maltose 90g / L, pH 5.8. Before culturing, the microspores were washed once with the medium, and then the microspore density was adjusted to 1.0-1.2×10 5 / ml, take 1.5ml of microspore suspension and inoculate it on a petri dish (30×15mm), seal it with Parafilm, and incubate in dark at 25°C for 21 days.

Embodiment 3

[0149] Embodiment 3: Acquisition of regenerated plants resistant to gibberellin virus

[0150] After 21 days of induction culture, the embryoid bodies were transferred to the differentiation medium. The differentiation medium used 2 / 3MS as the basic medium, added NaCl 300mg / L, 6-BA 0.5mg / L, KT 1.5mg / L, NAA 0.05mg / L and maltose 30g / L, and the pH was 5.8. Under the conditions of 25±1° C. and 10-12 hours of light per day, the differentiation stress culture is carried out for 25-30 days until green regenerated plants are differentiated.

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Abstract

The invention provides a method for screening anti-fusarium graminearum crude toxin of wheat crops. The method comprises the steps of: giving the fusarium graminearum crude toxin to the anther before microspore is cultivated in a stressing way, separating out the microspore from the anther which is stressed by the crude toxin, putting the microspore into a fusarium graminearum crude toxin-adding inductive medium to cultivate, and putting the microspore into a NaCl-adding differential medium to cultivate after the embryoid is obtained to obtain a regeneration plant. The whole screening processof the method is performed under a laboratory controllable condition, so that a screening result is real and reliable; and the resistance genes carried by the screened regeneration plant is homozygous with the other genes for once, and the controllable genetic stability of the genes is high.

Description

technical field [0001] The invention belongs to the technical field of crop genetics and breeding, and relates to a method for improving the scab resistance traits of wheat crops with seeds as the propagation mode. Background technique [0002] Wheat is the main food of our people, and barley is the main raw material for making beer. Wheat head blight can not only lead to a serious decline in yield, but more seriously, toxins such as deoxynivalenol produced by pathogenic bacteria accumulate in diseased wheat kernels, affecting human health. Due to the lack of effective control technology and the environmental pollution caused by chemical control, breeding resistant varieties is the most effective way to prevent wheat head blight. The practice of wheat head blight resistance breeding at home and abroad for many years has shown that the efficiency of traditional hybrid breeding techniques for breeding high-resistant materials is low, and a large number of hybrid progeny group...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 黄剑华陆瑞菊王亦菲陈志伟何婷
Owner SHANGHAI ACAD OF AGRI SCI
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