Culture medium prescription of regenerated plant from isolated microspore of brassica

A medium formula, a technology for regenerating plants, applied in plant regeneration, botanical equipment and methods, plant cells, etc., can solve the problems of unreasonable hormone concentration, low induced differentiation rate, high inorganic salt concentration, and shorten the breeding time. The effect of broad application prospect and high embryo rate

Inactive Publication Date: 2009-05-20
刘祥军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The traditional culture of microspores adopts MS and F medium, and MS and F medium have high inorganic salt concentration and high cost; and the concentration of hormones is unreasonable and the types are not complete, so the induced differentiation rate is low

Method used

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  • Culture medium prescription of regenerated plant from isolated microspore of brassica
  • Culture medium prescription of regenerated plant from isolated microspore of brassica

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The specific formula is shown in Table 1:

[0014] Table 1 The medium formula and sterilization method of free microspores of Brassica

[0015]

Embodiment 2

[0017] The specific formula is shown in Table 2:

[0018] Table 2 The medium formula and sterilization method of Brassica free microspore culture

[0019]

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PUM

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Abstract

The invention provides a culture medium formulation for regenerated plantlets of Brassica isolated microspores, which relates to improvement of a culture medium formulation for regenerated plantlets of Brassica microspores as horticultural crops, and belongs to the field of bioengineering. The invention provides the culture medium formulation for regenerated plantlets of Brassica isolated microspores, which is high in induced embryo formation rate and differentiation rate. A culture medium of the invention comprises an embryoid induction culture medium, a differentiation culture medium and a rooting culture medium, wherein the embryoid induction culture medium, the differentiation culture medium and the rooting culture medium all comprise macroelements, trace elements and organic elements. The formulation has the advantages of providing a large number of new genotype pure lines for breeding and greatly enriching breeding resources of Brassica.

Description

technical field [0001] The invention relates to the improvement of the medium formula of the microspore regeneration plant of the horticultural crop Brassica, and belongs to the field of bioengineering. Background technique [0002] Traditional microspore culture uses MS and F medium. MS and F medium have high inorganic salt concentration and high cost; and the concentration of hormones is unreasonable and the types are not complete, so the induced differentiation rate is low. Contents of the invention [0003] The present invention aims at the above problems and provides a medium formula for regenerating plants of Brassica genus free microspores with high embryogenesis rate and high differentiation rate. [0004] In order to achieve the above object, the present invention adopts the following technical scheme, the culture medium of the present invention includes embryoid body induction medium, differentiation medium and rooting medium, and described embryoid body inductio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 刘祥军
Owner 刘祥军
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