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Complex breeding method of high-chlorophyll multi-tiller barley

A technology of chlorophyll content, barley, applied in the field of agriculture

Active Publication Date: 2014-03-19
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The screening of barley breeding mostly focuses on the growth and maturity stage, and the screening of shoot biomass and chlorophyll content at the seedling stage has not been reported yet.

Method used

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  • Complex breeding method of high-chlorophyll multi-tiller barley

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Example 1: Parental Screening

[0096] According to the visual observation of the barley materials grown in the field, 200 materials with dark green leaves, vigorous growth at the seedling stage, and a large number of tillers were selected, and the seeds were harvested by individual plants. Take 100 seeds, soak the surface with 75% ethanol for 30 seconds, sterilize with 1% sodium hypochlorite for 15 minutes, rinse with distilled water, put them on wet gauze and germinate overnight in the dark at room temperature. 30 seedlings, wrap the seeds with sponge strips, place them in the round holes of the floating board (the floating board can be drilled with a foam board at a distance of 5 cm, and the diameter is about 1.5 cm), and cultivate them in the nutrient solution. The nutrient solution adopts the reported standard barley hydroponic nutrient solution (improved Hoagland solution, the formula is as follows:

[0097] NH 4 NO 3 (0.2mM);KNO 3 (5mM); CaNO 3 .2(2mM); MgSO...

Embodiment 2

[0101] Embodiment 2: screening parent planting

[0102] Select materials with one or more of the above excellent traits (high chlorophyll, multiple tillers, rapid growth) as parent materials for hybridization, and sow in the field that year. Select seeds with uniform size and full grains to sow in the field with a spacing of 3 cm and a row spacing of 30 cm. The soil contains 34.0g / kg of organic matter, 2.41g / kg of total nitrogen, and 37.30mg / kg of available nitrogen. Apply 20kg / mu of basal fertilizer before sowing, and 10kg / mu of urea at the tillering stage. During the period, samples can be taken to detect the leaf color and aboveground biological yield. .

Embodiment 3

[0103] Example 3: Hybridization

[0104] Two copies of the materials carrying different target traits (high chlorophyll or multi-tiller) are crossed forward and reverse. The specific method is to cut off the tassel glume of the female plant when the pollen is mature, and pollinate the floret pollen of the male plant, and carry out listing at the same time. Mark, and harvest hybrid seeds individually when mature.

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Abstract

The invention provides a complex breeding method of barley with high chlorophyll content and / or large tiller number, a method for preparing barley green products and a method for improving the chlorophyll content and / or the tiller number of the barley. The method provided by the invention comprises the following steps: selecting spikelets with barley microspores in development single-nuclear early stage and / or middle stage, and performing treatment at the temperature of 2-10 DEG C for 10-30 days; harvesting the microspores, culturing the microspores and regenerating to obtain a homozygous doubled haploid; culturing the homozygous doubled haploid; and further screening to obtain the barley with the high chlorophyll content and / or the large tiller number, and preparing the barley into the required barley green products. According to the method, a plurality of excellent traits are polymerized through a hybridization technology, a microspore culture technology is utilized to perform fast homozygous stabilization on target traits, multi-index screening is performed under an artificial controllable environment, and a barley germplasm material with high chlorophyll content and multiple tillers is finally obtained. The material can be used as the excellent germplasm material for producing the barley green and other nutritional and health care products in a field planting or water culture way.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and in particular relates to a method for screening and cultivating barley materials with high chlorophyll content and multiple stems. Background technique [0002] The seedlings of wheat crops are a kind of traditional Chinese herbal medicine. The medicinal value of wheat seedlings was recorded in "Compendium of Materia Medica" and "Treatise on Febrile Diseases" in my country. In 1969, Dr. Ogihara Yoshihide, a Japanese scholar, published the article "Research on Green Leaf Components of Grass Plants and As Stable Medicines and Foods" and devoted himself to commercial development. In 1991, he used the technology of "ultra-low temperature rapid spray drying" to concentrate the juice of young barley seedlings into a powder, which was called "Barely Green", and promoted it to the international market. The barley chlorophyll products currently on the market are made from the tender leaves (20-...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘成洪黄剑华王亦菲陈志伟陆瑞菊杜志钊何婷高润红徐红卫郭桂梅
Owner SHANGHAI ACAD OF AGRI SCI
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