Method for restoring new germplasm by rapidly creating male sterility of processing type hot pepper

Abstract

The invention discloses a method for restoring a new germplasm by rapidly creating male sterility of processing type hot pepper. The method comprises the following steps: placing a processing type hot pepper donor parent plant at the temperature of 26.4+ / -0.4 DEG C; performing anther culture and selecting small microspores which are in a mid-late uninucleate stage for inducing the generation of embryoid bodies; taking flower buds before 9 am every day, and performing low-temperature pre-treament on the flower buds for 1 to 3 days in a refrigerator at 4 DEG C; soaking in alcohol under an aseptic condition, then sterilizing, washing with sterile water, and taking anther out from the flower buds and inoculating to an induced culture medium by using tweezers; inoculating weight flower buds in a culture vessel; after the anther is taken out from the flower buds under the sterile condition, uniformly dispersing the anther on the induced culture medium; transferring the induced embryoid bodies to an MS culture medium; when the root length reaches 4 to 5 cm, transplanting, wherein seedling hardening is required to be performed before transplanting. According to the method, the anther culture work is carried out more effectively and conveniently; morphological indexes of sampling flower buds are mastered; operation links are reduced; the anther culture induction efficiency is ensured.

Description

technical field [0001] The invention belongs to the technical field of anther culture, and in particular relates to a method for quickly creating a new germplasm of processed pepper male sterility restoration. Background technique [0002] At present, anther culture has become the main technology of capsicum breeding. In the past 20 years, some fine pepper cultivars have been bred, such as Haihua No. 3 (Li Chunling et al., 1990), Haifeng No. 12 (Xing Yongping et al., 2002), Haifeng No. 26 (Xing Yongping et al., 2003), Haifeng No. 14 (Zhang Shugen et al., 2003), Haifeng No. 25 (Zhang Shugen et al., 2008), Haifeng No. 16 (Xing Yongping et al., 2014), Haifeng No. 1052 (Zhang Shugen et al., 2015) and Haifeng 391 (Zhang Shugen et al., 2015) and so on. Homozygous diploid materials can be obtained in 1 to 2 years through anther culture technology, which can speed up the breeding process and shorten the time required for breeding (Chen Xiao et al., 2003; Yuan Li et al., 2011). Mo...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for quickly creating a new germplasm for restoring male sterility of processed peppers, aiming at solving the problem that there are few excellent restorer lines of CMS in processed peppers in breeding practice, and the CMS restoration of processed peppers is difficult in traditional breeding. Time-consuming and labor-intensive problem

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