Trichoderma spore powder as well as preparation method and application thereof
A technology of spore powder and spore powder, which is applied in the field of Trichoderma spore powder and its preparation, can solve the problems of unreasonable application and maintenance of microbial activity, and achieve the effects of easy promotion, high density of Trichoderma spores, and significant antagonistic effect
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Embodiment 1
[0029] 1. Recovery and expansion of strains
[0030] (1) The refrigerated Trichoderma asperellum T1 (Trichoderma asperellum T 1 ) is planted on the PDA medium to make it recover;
[0031] (2) Adopting yeast extract sucrose medium, and setting the culture temperature as 30°C and humidity as 70-85%, the bacterial strain recovered in step (1) is subcultured for 48-72 hours on a flat plate until it grows A dark green colony with a diameter of about 5-7mm was produced;
[0032] (3) Wash the bacterium colonies formed by the plate subculture in step (2) with sterile water, and inoculate them in an eggplant-shaped bottle filled with high-temperature sterilized compost consisting of bran, sawdust, sucrose, vitamin B and water Carry out the spore expansion culture in the medium, set the culture temperature at 30°C, the humidity at 70-85%, and the culture time at 5-7 days. Turn and beat the eggplant-shaped bottle every day until white hyphae grow on the culture and grow on it. Green s...
Embodiment 2
[0039] The impact of changes in parameters such as air inlet temperature, feed amount, type of drying aid, and content of drying aid on the preparation of Trichoderma spore powder will be further described below.
[0040] The germination rate is used as an index to do single factor analysis on the air inlet temperature and feed amount of spray drying, and the influence of different inlet air temperature and feed amount on the prepared spore powder is measured by changing the inlet air temperature and feed amount during the preparation process. In order to compare and select the range of air inlet temperature and feed amount that is beneficial to ensure high spore germination rate and good drying effect. When feed rate is 5wt%, set 90 ℃, 100 ℃, five inlet air temperatures such as 110 ℃, 120 ℃ and 130 ℃, measure the germination rate of the prepared spore powder of each temperature and record drying effect; When the wind temperature is 120°C, set five feeding amounts such as 1wt%...
Embodiment 3
[0049] The L9 (3 4 ) Orthogonal test, with the germination rate, collection rate, pollen collection rate and live spore content of the product as evaluation indicators, to select a suitable preparation process. In this experiment, L9 (3 4 ) Orthogonal test, as shown in Table 3 and 4:
[0050] Table 3 Factor level table of orthogonal test for optimization of spore powder formula
[0051]
[0052] Table 4 Orthogonal test results of spore powder formulation process
[0053]
[0054]
[0055] Note:
[0056]
[0057] It can be seen from Table 4 that in A 1 B 1 C 1 D. 2 Under the formulation process, that is, 5wt% β-cyclodextrin, the inlet air temperature is 110°C, and the feed amount is 10wt%, the content of live spores in the product is the highest. By comparing the range (R) of the index, the primary and secondary order of the factors affecting the live spore content is A>C>D>B, that is, the type of drying agent is the main influencing factor, and the secon...
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