Paecilomyces lilacinus and application thereof

A technology of Paecilomyces lilacinus and inoculum agent, applied to Paecilomyces lilacinus and application fields, can solve the problems of lack of preparations, extensive process, poor soil applicability and the like

A technology of Paecilomyces lilacinus and inoculum agent, applied to Paecilomyces lilacinus and application fields, can solve the problems of lack of preparations, extensive process, poor soil applicability and the like

CN101100646AInactive Publication Date: 2008-01-09INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Paecilomyces lilacinus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1, Isolation and Preservation of Paecilomyces lilacinus (P.lilacinus) YES-2 CGMCC No.2012

[0059] Collect roots of tobacco root-knot nematode disease in Tadian, Eshan, Yunnan, wash the diseased roots with tap water for 3-5 minutes to remove the soil, then put the diseased roots under a dissecting microscope, pick out the oocysts with tweezers, and treat the oocysts with 1% sodium hypochlorite for 1 minute Disinfect the surface of oocysts and release nematode eggs, pass the sodium hypochlorite egg suspension through 200 μm and 30 μm mesh sieves respectively, rinse 3 times with sterilized distilled water to remove sodium hypochlorite, spread approximately every 100 eggs on a PDA plate, and observe the colonies every day The situation arises. Finally, a nematicidal fungal strain was isolated from the eggs of root-knot nematode tobacco, named YES-2, which was deposited in the China Microorganism Culture Collection Management Committee located in Datun Road, Chaoyan...

Embodiment 2

[0060] Embodiment 2, the cultivation of Paecilomyces lilacinus (P.lilacinus) YES-2 CGMCC No.2012 and the preparation of its bacterial agent

[0061] 1. Cultivation of Paecilomyces lilacinus (P.lilacinus) YES-2 CGMCC No.2012

[0062] 1. Test tube culture

[0063] Potato agar medium formula: 200g potato, 20g glucose, 16g agar, dilute to 1L with water, pH6-7.

[0064] Paecilomyces lilacinus YES-2 was inoculated on a potato agar medium in a test tube, and cultured at 25° C. for 72-96 hours to obtain activated Paecilomyces lilacinus YES-2.

[0065] 2. Expand training

[0066] Paecilomyces lilacinus (P.lilacinus) YES-2 CGMCC No.2012 special liquid medium formula: maltose (C source) 30g, yeast (N source) 6.12g, K 2 HPO 4 1g, MgSO 4 0.5g, KCl0.5g, FeSO 4 0.01g, ZnSO 4 ·7H 2 O 10mg, dilute to 1L with water, pH6-7.

[0067] Inoculate the Paecilomyces lilacinus YES-2 activated in step 1 into the special liquid medium for Paecilomyces lilacinus YES-2, and culture it at 25° C. and...

Embodiment 3

[0076] Embodiment 3, the cultivation of Paecilomyces lilacinus (P.lilacinus) YES-2 CGMCC No.2012 and the preparation of its bacterial agent

[0077] 1. The cultivation of Paecilomyces lilacinus YES-2

[0078] 1. Test tube culture

[0079] Potato agar medium formula: 200g potato, 20g glucose, 16g agar, dilute to 1L with water, pH6-7.

[0080] Paecilomyces lilacinus YES-2 was inoculated on a potato agar medium in a test tube, and cultured at 25° C. for 72 hours to obtain activated Paecilomyces lilacinus YES-2.

[0081] 2. Expand training

[0082] Formula of special liquid medium for Paecilomyces lilacinus YES-2: maltose 20g, yeast 5g, K 2 HPO 4 0.5g, MgSO 4 0.1g, KCl0.1g, FeSO 4 0.001g, ZnSO 4 ·7H 2 O5mg, dilute to 1L with water, pH6-7.

[0083] Inoculate the Paecilomyces lilacinus YES-2 activated in step 1 into the special liquid medium for Paecilomyces lilacinus YES-2, and culture it at 26° C. and 180 rpm for 150 h, and the Paecilomyces lilacinus YES-2 is expanded.

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Abstract

A (P.lilacinus)YES-2 CGMCC No. 2012, its bacterial agent and use are disclosed. The process is carried out by taking polymer sodium alginate as base skeleton, taking (P.lilacinus)YES-2 CGMCC No. 2012 mycelium and spore mixture as active ingredients, taking diatomite as carrier, forming sodium alginate and polyvalent positive ion into gel and sodium alginate-calcium chloride reacting to obtain final product. The prevention rate reaches to 50-60%. It's stable, cheap and convenient. It has no chemical residual and pollution for agricultural product and environment. It can be used to prevent plant root nematode.

Description

technical field [0001] The invention relates to a strain of Paecilomyces lilacinus and its application, in particular to a strain of Paecilomyces lilacinus and its agent and the application of the agent in preventing and treating plant root-knot nematode diseases. Background technique [0002] Among the four major pathogens that cause plant infectious diseases, plant parasitic nematodes are more harmful than bacteria and viruses, and are second only to fungal diseases. Plant parasitic nematodes cause about 100 billion US dollars of losses in world agricultural production every year, among which root-knot nematodes (Meloidogyne spp.) are the most harmful type of plant parasitic nematodes, and the economic losses caused by the world's important economic crops are as high as tens of millions of dollars each year. tens of billions of dollars. Within the genus Meloidogyne, M. incognita, M. arenaria, M. javanica and M. hapla The root-knot nematodes of the four species are the mo...

Claims

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Application Information

Patent Timeline
09 Jan 2008
Publication
CN101100646A
IPC
C12N1/14; C12N11/14; A01N63/04; A01P5/00; C12R1/79
Inventors
段维军; 刘杏忠