Paecilomyces lilacinus and application thereof
A technology of Paecilomyces lilacinus and inoculum agent, applied to Paecilomyces lilacinus and application fields, can solve the problems of lack of preparations, extensive process, poor soil applicability and the like
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Embodiment 1
[0058] Example 1, Isolation and Preservation of Paecilomyces lilacinus (P.lilacinus) YES-2 CGMCC No.2012
[0059] Collect roots of tobacco root-knot nematode disease in Tadian, Eshan, Yunnan, wash the diseased roots with tap water for 3-5 minutes to remove the soil, then put the diseased roots under a dissecting microscope, pick out the oocysts with tweezers, and treat the oocysts with 1% sodium hypochlorite for 1 minute Disinfect the surface of oocysts and release nematode eggs, pass the sodium hypochlorite egg suspension through 200 μm and 30 μm mesh sieves respectively, rinse 3 times with sterilized distilled water to remove sodium hypochlorite, spread approximately every 100 eggs on a PDA plate, and observe the colonies every day The situation arises. Finally, a nematicidal fungal strain was isolated from the eggs of root-knot nematode tobacco, named YES-2, which was deposited in the China Microorganism Culture Collection Management Committee located in Datun Road, Chaoyan...
Embodiment 2
[0060] Embodiment 2, the cultivation of Paecilomyces lilacinus (P.lilacinus) YES-2 CGMCC No.2012 and the preparation of its bacterial agent
[0061] 1. Cultivation of Paecilomyces lilacinus (P.lilacinus) YES-2 CGMCC No.2012
[0062] 1. Test tube culture
[0063] Potato agar medium formula: 200g potato, 20g glucose, 16g agar, dilute to 1L with water, pH6-7.
[0064] Paecilomyces lilacinus YES-2 was inoculated on a potato agar medium in a test tube, and cultured at 25° C. for 72-96 hours to obtain activated Paecilomyces lilacinus YES-2.
[0065] 2. Expand training
[0066] Paecilomyces lilacinus (P.lilacinus) YES-2 CGMCC No.2012 special liquid medium formula: maltose (C source) 30g, yeast (N source) 6.12g, K 2 HPO 4 1g, MgSO 4 0.5g, KCl0.5g, FeSO 4 0.01g, ZnSO 4 ·7H 2 O 10mg, dilute to 1L with water, pH6-7.
[0067] Inoculate the Paecilomyces lilacinus YES-2 activated in step 1 into the special liquid medium for Paecilomyces lilacinus YES-2, and culture it at 25° C. and...
Embodiment 3
[0076] Embodiment 3, the cultivation of Paecilomyces lilacinus (P.lilacinus) YES-2 CGMCC No.2012 and the preparation of its bacterial agent
[0077] 1. The cultivation of Paecilomyces lilacinus YES-2
[0078] 1. Test tube culture
[0079] Potato agar medium formula: 200g potato, 20g glucose, 16g agar, dilute to 1L with water, pH6-7.
[0080] Paecilomyces lilacinus YES-2 was inoculated on a potato agar medium in a test tube, and cultured at 25° C. for 72 hours to obtain activated Paecilomyces lilacinus YES-2.
[0081] 2. Expand training
[0082] Formula of special liquid medium for Paecilomyces lilacinus YES-2: maltose 20g, yeast 5g, K 2 HPO 4 0.5g, MgSO 4 0.1g, KCl0.1g, FeSO 4 0.001g, ZnSO 4 ·7H 2 O5mg, dilute to 1L with water, pH6-7.
[0083] Inoculate the Paecilomyces lilacinus YES-2 activated in step 1 into the special liquid medium for Paecilomyces lilacinus YES-2, and culture it at 26° C. and 180 rpm for 150 h, and the Paecilomyces lilacinus YES-2 is expanded.
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