Tissue culture disinfection and sterilization formula of ormosia henryi prain and tissue culture method of ormosia henryi prain

Abstract

The invention discloses a tissue culture disinfection and sterilization formula of ormosia henryi prain and a tissue culture method of the ormosia henryi prain. Seed seedling stems are disinfected by 70% ethanol for 30 seconds, sterilized by 0.1% mercury bichloride for 8 minutes, disinfected by the 70% ethanol for 30 seconds and treated by 0.5ml/L of a plant tissue culture antibacterial agent to achieve the best disinfection effect; the optimal collection time of seedling explants is April; the stems are immersed by the 70% ethanol for 30 seconds, immersed by a mixed solution (containing 50mg/L rifampicin and 50mg/L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 8 minutes to achieve the best disinfection effect; and leaves are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg/L rifampicin and 50mg/L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 6 minutes to achieve the best disinfection effect. Leaves of grown plants are relatively suitable for collecting in April, and are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg/L rifampicin and 50mg/L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 12 minutes. According to the tissue culture disinfection and sterilization formula and the tissue culture method, seed seedlings, seedlings and grown plant tissues of ormosia henryi prain explants are disinfected by different disinfection methods, and the relatively good survival rate can be obtained; and sources of ormosia henryi prain tissue culture multiplication materials are greatly expanded, and the tissue culture effect of the ormosia henryi prain can be greatly improved by controlling disinfection time.

Description

Technical field [0001] The invention belongs to the technical field of tissue culture, and specifically relates to a sterilization formula for tissue culture of palm tree and a method for tissue culture of palm tree. Background technique [0002] Palmwood ( Ormosiahenryi Prain) is an evergreen tree of the leguminous red bean genus, also known as rosewood and odorifera. It is a national secondary key protected plant. It is 5-13 meters high and has odd-pinnate compound leaves, oblong or oblong oblanceolate. Evergreen in all seasons, no obvious deciduous phenomenon; branchlets and leaf backs are densely gray-yellow pubescent; flowering period from June to July, yellow-white, raceme-like florets; pods are flat, dark brown when mature, and seeds are red. The wood of palm tree is dense, beautiful in texture, shiny, corrosion-resistant, easy to cut, and smooth in cut surface. It is a precious timber species. Because of its smooth bark and beautiful appearance, it has become a high-qual...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a sterilizing formula and a tissue culture method for the tissue culture of japonica, aiming at solving the problems of serious pollution, extremely low survival rate and difficulty in induction in the tissue culture of japonica explants, so as to Overcome the deficiencies of existing technical problems

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