Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tissue culture disinfection and sterilization formula of ormosia henryi prain and tissue culture method of ormosia henryi prain

A technology for disinfection and sterilization, and flower palm wood, which is applied in the fields of botanical equipment and methods, horticultural methods, horticulture, etc., to achieve the effects of reducing the pollution rate, achieving remarkable tissue culture effect, and improving tissue culture survival rate.

Inactive Publication Date: 2015-12-23
GUIZHOU UNIV
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a sterilizing formula and a tissue culture method for the tissue culture of japonica, aiming at solving the problems of serious pollution, extremely low survival rate and difficulty in induction in the tissue culture of japonica explants, so as to Overcome the deficiencies of existing technical problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture disinfection and sterilization formula of ormosia henryi prain and tissue culture method of ormosia henryi prain
  • Tissue culture disinfection and sterilization formula of ormosia henryi prain and tissue culture method of ormosia henryi prain
  • Tissue culture disinfection and sterilization formula of ormosia henryi prain and tissue culture method of ormosia henryi prain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: A disinfection and sterilization formula for tissue culture of palm tree, including 70% alcohol, 0.1% mercury liters, 70% alcohol and adding 0.5 ml / L of plant tissue culture antibacterial agent to the value-added medium.

Embodiment 2

[0057] Example 2: A disinfection and sterilization formula for tissue culture of palm tree, including 70% alcohol, 50mg / L rifampicin and 50mg / L chloramphenicol mixture, and 0.1% mercury.

Embodiment 3

[0058] Example 3: A disinfection and sterilization formula for tissue culture of palm tree, including 70% alcohol, a mixture of 50 mg / L rifampicin and 50 mg / L chloramphenicol, and 0.1% mercury.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a tissue culture disinfection and sterilization formula of ormosia henryi prain and a tissue culture method of the ormosia henryi prain. Seed seedling stems are disinfected by 70% ethanol for 30 seconds, sterilized by 0.1% mercury bichloride for 8 minutes, disinfected by the 70% ethanol for 30 seconds and treated by 0.5ml / L of a plant tissue culture antibacterial agent to achieve the best disinfection effect; the optimal collection time of seedling explants is April; the stems are immersed by the 70% ethanol for 30 seconds, immersed by a mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 8 minutes to achieve the best disinfection effect; and leaves are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 6 minutes to achieve the best disinfection effect. Leaves of grown plants are relatively suitable for collecting in April, and are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 12 minutes. According to the tissue culture disinfection and sterilization formula and the tissue culture method, seed seedlings, seedlings and grown plant tissues of ormosia henryi prain explants are disinfected by different disinfection methods, and the relatively good survival rate can be obtained; and sources of ormosia henryi prain tissue culture multiplication materials are greatly expanded, and the tissue culture effect of the ormosia henryi prain can be greatly improved by controlling disinfection time.

Description

Technical field [0001] The invention belongs to the technical field of tissue culture, and specifically relates to a sterilization formula for tissue culture of palm tree and a method for tissue culture of palm tree. Background technique [0002] Palmwood ( Ormosiahenryi Prain) is an evergreen tree of the leguminous red bean genus, also known as rosewood and odorifera. It is a national secondary key protected plant. It is 5-13 meters high and has odd-pinnate compound leaves, oblong or oblong oblanceolate. Evergreen in all seasons, no obvious deciduous phenomenon; branchlets and leaf backs are densely gray-yellow pubescent; flowering period from June to July, yellow-white, raceme-like florets; pods are flat, dark brown when mature, and seeds are red. The wood of palm tree is dense, beautiful in texture, shiny, corrosion-resistant, easy to cut, and smooth in cut surface. It is a precious timber species. Because of its smooth bark and beautiful appearance, it has become a high-qual...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 韦小丽乔栋张兰安常蓉
Owner GUIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products