A kind of tissue culture disinfection and sterilization formula and tissue culture method of flower palm wood

Abstract

The invention discloses a tissue culture disinfection and sterilization formula of ormosia henryi prain and a tissue culture method of the ormosia henryi prain. Seed seedling stems are disinfected by 70% ethanol for 30 seconds, sterilized by 0.1% mercury bichloride for 8 minutes, disinfected by the 70% ethanol for 30 seconds and treated by 0.5ml / L of a plant tissue culture antibacterial agent to achieve the best disinfection effect; the optimal collection time of seedling explants is April; the stems are immersed by the 70% ethanol for 30 seconds, immersed by a mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 8 minutes to achieve the best disinfection effect; and leaves are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 6 minutes to achieve the best disinfection effect. Leaves of grown plants are relatively suitable for collecting in April, and are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 12 minutes. According to the tissue culture disinfection and sterilization formula and the tissue culture method, seed seedlings, seedlings and grown plant tissues of ormosia henryi prain explants are disinfected by different disinfection methods, and the relatively good survival rate can be obtained; and sources of ormosia henryi prain tissue culture multiplication materials are greatly expanded, and the tissue culture effect of the ormosia henryi prain can be greatly improved by controlling disinfection time.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a formula for disinfecting and sterilizing tissue culture of japonica, and also relates to a tissue culture method of japonica. Background technique [0002] Flower palm (Ormosia henryi Prain) is an evergreen tree belonging to the family Fabaceae ormosia, also known as rosewood and stinky tung wood. It is a national second-class key protected plant. Oblong and oblanceolate, evergreen in four seasons, without obvious leaf fall; branchlets and leaf backs are densely grayish-yellow pubescent; flowering period is June-July, yellowish-white, racemes; pods are flat, dark brown when mature, red seeds . The palm wood is dense, beautiful in texture, shiny, corrosion-resistant, easy to cut, and smooth in cut surface. It is a precious timber tree species, and because of its green and smooth bark, it has become a high-quality landscaping tree species, suitable for street ...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a sterilizing formula and a tissue culture method for the tissue culture of japonica, aiming at solving the problems of serious pollution, extremely low survival rate and difficulty in induction in the tissue culture of japonica explants, so as to Overcome the deficiencies of existing technical problems

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