Cinnamomum camphora tissue culture method

A technology of tissue culture and borneol camphor, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of seedling variation, limited number of good-type seedling breeding, and low survival rate of transplanting

Inactive Publication Date: 2015-07-01
江西北辰德天然生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The breeding of borneol camphor usually includes seed propagation and cutting propagation. Because there are very few naturally distributed borneol camphor trees, there are variations in seedlings after sowing and propagation; cutting propagation also results in a very limited number of good-type seedlings due to the lack of good-type resources.
Therefore, if the method of tissue culture can be used to propagate the excellent type seedlings of borneol camphor, it will be possible to better solve the problems of slow propagation speed of excellent seedlings of borneol camphor and low survival rate of transplanting.

Method used

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  • Cinnamomum camphora tissue culture method
  • Cinnamomum camphora tissue culture method

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Embodiment 1

[0021] In the embodiment of the present invention, the material is taken from the fresh semi-lignified branches of the excellent type of borneol camphor in Ji'an City, and the time of collection is April 30. experiment method:

[0022] (1) Culture conditions

[0023] Both MS basic medium and improved MS medium contain 3% sucrose, 0.8% carrageenan, pH 5.5, placed at a temperature of 26-28°C, light intensity of 1500-2000lx, and cultivated under light for 12 hours a day.

[0024] (2) Proliferation and culture research

[0025] In the following research experiments, experiments 1, 2, 3, and 4 were each treated with 200 replicates, each replicated with 1 multiplication unit (1 stem segment with 2-3 nodes). Each proliferation cycle is 35 days.

[0026] 1. Effect of different pH value medium on proliferation

[0027] The medium for the two treatments used the modified MS + 6-BA (6-benzylaminoadenine) 0.8 mg / L + NAA (naphthalene acetic acid) 0.05 mg / L medium. However, when prepar...

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Abstract

The invention discloses a cinnamomum camphora tissue culture method. The method comprises the following steps: picking new sprouted semi-lignified branches of cinnamomum camphora in the same year in April to May each year, scissoring branches with 2-3 leaves, removing the leaves, oscillating and washing in sterile water for 3-5 minutes, treating by using 70% alcohol for 1-2 minutes, placing in 0.1% mercury bichloride for 6-10 minutes, and cleaning by using sterile water for 3 times; inoculating the treated semi-lignified branches into an adventitious bud induction medium, thereby obtaining an aseptic explant, culturing under illumination conditions, and thus forming callus and adventitious buds for expanding propagation; culturing in a mulitiplication expanding propagation culture medium and a root induction medium, and cultivating to obtain rooting seedlings; and transplanting the cultured cinnamomum camphora rooting seedlings, wherein the survival rate is over 95 percent.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a borneol camphor tissue culture method. Background technique [0002] Camphor tree (Cinnamomum comphora) is an evergreen tree species of the genus Cinnamon in the family Lauraceae, and it is a native species in Ji'an City. After years of research, researchers from the Ji'an Forestry Institute have classified camphor trees into borneol camphor, cinnamon camphor, camphor camphor, oil camphor, and different camphor trees according to the chemical components contained in camphor trees. Among the above chemical types, borneol camphor is the tree species with the least natural distribution and the highest economic value. The natural d-borneol camphor oil extracted through borneol coppice plantation is widely used in industries such as medicine, flavors and fragrances and cosmetics, and has a broad market prospect. [0003] The breeding of borneol camphor usually includes seed propagation a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 胡庆彭建军
Owner 江西北辰德天然生物科技有限公司
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