Method for breeding spring cabbage stalk and maintaining springness of inbred line

A technology of head cabbage and inbred lines, applied in horticultural methods, botany equipment and methods, plant cells, etc., can solve the problems of plant death, breeding obstacles, heading, low temperature resistance, poor light resistance, etc. Achieve the effect of increasing the number of genotype plants

Inactive Publication Date: 2009-07-08
NORTHWEST A & F UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heading cabbage is a typical green-body vernalized biennial crop. Only when the plant vegetative body reaches a certain volume and undergoes a certain low temperature induction can it pass vernalization. Therefore, only one growth period can be completed in a year, which is suitable for early-maturing spring crops that are suitable for spring cultivation. The selection of hybrid first-generation varieties and their parents is extremely unfavorable and brings breeding obstacles, making it difficult to breed spring cabbage cultivated in spring
If the parental inbred lines of head cabbage cultivated in spring are often screened in autumn, the spring sex of the cultivated varieties in spring will be seriously degraded, and the adaptability of spring head cabbage varieties will be weakened in spring, such as easy to show head head and low temperature tolerance. , poor resistance to low light, and prone to early bolting
Although scientists have researched that the method of propagating axillary bud cuttings of head cabbage in spring can retain the spring nature of parental inbred lines, the cultivation period of axillary bud cutting seedlings is long. It is very low, and the cost is also high; even if the surviving seedlings are cultivated in autumn, the seedlings will be too long, the plants will be aging, and the damage of autumn diseases will cause the plants to die prematurely, and the inbred line materials selected for breeding will be lost.
This has just had a strong impact on the breeding process of spring cabbage suitable for spring cultivars

Method used

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  • Method for breeding spring cabbage stalk and maintaining springness of inbred line
  • Method for breeding spring cabbage stalk and maintaining springness of inbred line
  • Method for breeding spring cabbage stalk and maintaining springness of inbred line

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preparation example Construction

[0030] 3) Preparation of root medium for adventitious bud induction

[0031] The formula of the adventitious bud induction root medium is: add 3% sucrose (mass), 0.8% agar (mass) and 0.5-1.0mg / L alpha-naphthaleneacetic acid (NAA) to MS medium.

[0032] 4) Differentiation induction of adventitious buds

[0033] Inoculate the explants on the adventitious bud differentiation induction medium in the culture bottle, cultivate them at a temperature of 25°C, light for 12h-14h per day, and a light intensity of 2000-2500Lx, and induce adventitious buds after 20-30 days (d) .

[0034] 5) rooting induction of adventitious buds

[0035] When the adventitious buds grow to a height of 1.5-2.5 cm, cut them from the base of the adventitious buds and transfer them to the medium for inducing adventitious buds in a culture bottle, at a temperature of 25°C, light for 10h-12h a day, and the light intensity is 2000-2500Lx Cultivate under certain conditions, induce rooting after 15-20 days (d), a...

Embodiment 1

[0041] This embodiment selects the early generation of the male parent (inbred line YCF51-99-835168) of the spring cabbage variety "Qingan 50" (national variety identification) newly bred by the applicant's spring cabbage breeding laboratory as the experimental material .

[0042] 1) Selection of explants

[0043] The early generation of spring cabbage inbred line YCF51-99-835168 was raised in a cold bed on December 20, and the seedlings were separated once with 3 true leaves. When the seedlings had 6-7 true leaves, they were planted in mid-March. open field. The field is normally managed according to the open-field cultivation of spring cabbage. When the plant grows and the leaf balls are mature ( Picture 1-1 ), the field selects plants that meet the breeding objectives and have excellent agronomic traits ( Figure 1-2 ), select its leaf bulb as explants for plant regeneration in vitro culture ( Figure 1-3 ).

[0044] 2) Induction culture of regenerated plants

[0045]...

Embodiment 2

[0054] This embodiment selects the early generation of the female parent (inbred line BMP01-88-561336) of the spring cabbage variety "Qingan 50" (national variety identification) newly bred by the applicant's spring cabbage breeding laboratory as the experimental material .

[0055] 1) Selection of explants

[0056] The early generation field culture and explant selection method of the female parent inbred line BMP01-88-561336 is the same as the early generation of the male parent inbred line YCF51-99-835168 (see Example 1).

[0057] 2) Induction culture of regenerated plants

[0058] ①Induction of adventitious buds

[0059] The outer green leaves of the early-generation leaf balls of the selected spring cabbage inbred line BMP01-88-561336 were selected as explants for in vitro culture. Collect the leaves and bring them back to the laboratory, rinse them with running water for 3 times, then soak them in 75% alcohol for 50 seconds (s), disinfect them with 0.1% mercury liter ...

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Abstract

The invention discloses a propagation method for keeping springness of an inbred line of a spring common head cabbage breed. The method comprises the following steps: selecting external-layer green leaves of mature leaf heads of the spring common head cabbage inbred line in spring, inoculating the leaves on an adventitious bud differentiation induction culture medium according to a certain size for culture, and inoculating adventitious buds to an adventitious root differentiation induction culture medium for culture when the adventitious buds grow to 1.5-2.5cm high to obtain complete regenerated seedlings; then transplanting the regenerated seedlings to culture soil for culture, and permanently planting the seedlings in a field (open field) when the seedlings grow to 5-7 true leaves and are adaptively hardened for 7 days, and by which the survival rate of the seedlings reaches over 95%. The method helps improve the breeding effect of the spring common head cabbage, ensure the spring common head cabbage inbred line to complete a heading stage in spring and agronomic character selection of plants of the spring common head cabbage, maintain the characters (springness) of the selected plants unchanged, ensure the inbred line to complete a life cycle, greatly improve the number of the plants with the same genotype of the inbred line, and proliferate the number of the plants over 50 times.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and relates to a method for realizing spring variety breeding of crops by cultivating biennial plant tissues, in particular to a breeding method for spring cabbage breeding to maintain the spring sex of inbred lines. Background technique [0002] Heading cabbage (Brassica oleracea.L, Var.capitata L.) is an important vegetable in the genus Brassica, Brassicaceae. It has the characteristics of high quality, rich nutrition, edible variety, storage and transportation resistance, and is widely cultivated all over the world. According to reports, in 2006, the sown area of ​​head cabbage in China reached 937,300 hm 2 , occupies an important position in domestic vegetable cultivation and annual supply. Heading cabbage has strong adaptability, and can be divided into spring, summer, autumn and other multi-seasons and multi-crop listings within a year, which plays a key role in alleviating the off-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 张恩慧许忠民杨安平程永安张高翔马青山李宏伟朱守亮
Owner NORTHWEST A & F UNIV
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