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Method for regenerating plants of Chinese rose by using leaves as explants

A technology of explants and plants, applied in the direction of plant regeneration, botany equipment and methods, horticultural methods, etc., to achieve good multiplication effect

Inactive Publication Date: 2011-01-19
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the induction of somatic embryos in rose 'Samansha' at home and abroad.

Method used

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  • Method for regenerating plants of Chinese rose by using leaves as explants
  • Method for regenerating plants of Chinese rose by using leaves as explants
  • Method for regenerating plants of Chinese rose by using leaves as explants

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Test material selection, medium design and inoculation culture

[0034] 1. The source and treatment of test materials:

[0035] The test material among the present invention is selected from the unexpanded leaves of Chinese rose 'Samansa', collected from the flower test base of Huazhong Agricultural University in Wuhan City, Hubei Province, and adopts the unexpanded leaves of the excellent Chinese rose 'Samansa' without damage by diseases and insect pests. Compound leaves. The original source of this variety is a public sale variety purchased from the Hubei Provincial Flower and Seedling Market on Xiongchu Street, Wuchang, Wuhan City, Hubei Province. The unexpanded compound leaves of rose 'Samansha' collected in the field were sterilized by conventional methods (Gao Liping. Establishment of plant regeneration system of rose variety 'Samansha' and research on genetic transformation mediated by Agrobacterium tumefaciens. [Ph.D. Dissertation ].Wuhan: Huazhong Agricultura...

Embodiment 2

[0050] Effects of Different Concentrations of 2,4-D on Somatic Embryogenesis of Rose 'Samansha'

[0051] In plant tissue culture, 2,4-D is a widely used auxin growth regulator, and it is recognized as the most effective auxin for initiating cell dedifferentiation, forming callus or organogenesis . The experimental results of different concentrations of 2,4-D on the somatic embryogenesis of rose 'Samansha' are shown in Table 2: when the concentration of 2,4-D is 0-2 mg / L, the petiole of the leaflet expands, and at Browning and death during the continuous culture; when the concentration of 2,4-D was 3mg / L and 4mg / L, somatic embryogenesis occurred at the petiole of the leaflet; when the concentration of 2,4-D was 5-8mg / L, The small leaves have adventitious roots. Therefore, 3mg / L 2,4-D is the optimal hormone concentration for the induction of somatic embryos in rose 'Samansha'.

[0052] Table 2 Effects of different concentrations of 2,4-D on somatic embryogenesis of rose 'Sama...

Embodiment 3

[0055] Effects of different concentration combinations of 2, 4-D and 6-BA on the growth of somatic embryos in rose 'Samansha'

[0056] Somatic embryos were inoculated on MS medium containing different concentrations of 2,4-D (0, 0.5, 1.0 mg / L) and 6-6-BA (0, 0.05 mg / L), and the glucose concentration was 60 g / L , the plant gel is 2.5g / L. Cultivate under light conditions. After 4 weeks, the effects of exogenous hormones on the growth status of somatic embryos were compared. The results are shown in Table 3: when the concentration of 2,4-D was too low, the growth potential of the somatic embryos was weak, the proliferation was slow and the callus was serious. Somatic embryos grow best on the medium containing 1.0mg / L 2,4-D, 0.05mg / L 6-BA, somatic embryos grow vigorously, proliferate obviously and rapidly, and the proportion of somatic embryo tissue reaches 90% Therefore, it is suitable for the proliferation and preservation of rose somatic embryos.

[0057] Table 3 Effects of...

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Abstract

The invention belongs to the technical field of plant tissue and cell culture, and in particular relates to a plant regeneration method by using leaves of Chinese rose as explants through a somatic cell embryogenesis path. The method comprises the following three culture stages: (1) culturing the Chinese rose explants on an induction medium, and directly inducing somatic embryos from the explants, wherein the explants are wrapped leaves of Chinese rose respectively; (2) inoculating the inoculate on a propagation culture medium for propagation and accelerating further differentiation thereof; and (3) inoculating the somatic embryos subjected to propagation culture to a somatic embryo seedling culture medium and a rooting culture medium and regenerating complete plants. The method has the advantages of wide sources of explants, convenient material selection and the like. The prepared somatic embryos are agrobacterium-mediated excellent receptors through genetic transformation and can be used for research on genetic transformation of the Chinese rose. The invention also relates to components of culture media for the method.

Description

technical field [0001] The invention belongs to the technical field of plant tissue and cell culture, and specifically relates to a method for regenerating rose plants through somatic embryogenesis by using rose leaves as explants. Background technique [0002] Rosa hybrida is a perennial evergreen woody plant belonging to the genus Rosa of the family Rosaceae. Rose has a long history of cultivation and is widely planted by countries all over the world. It is a very important ornamental flower with important commercial value and plays an important role in landscaping. Rose is known as the queen of flowers because of its graceful posture, rich and gorgeous flower colors, rich fragrance, long flowering period, strong adaptability, easy reproduction and convenient management. Roses can be used in a wide range of ways. For example, vine roses can be used to decorate corridors and arches, tree roses can be used to decorate main roads, shrub roses can be used to make hedges, poly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 包满珠刘国锋包颖邢文张俊卫宁国贵傅小鹏
Owner HUAZHONG AGRI UNIV
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