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Electroporation conversion method for breeding high-temperature pleuratus ferulae

A technology of electro-excitation transformation and Asafoetida mushrooms is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of shortening the growth cycle, saving cultivation costs, and excellent biological characteristics.

Inactive Publication Date: 2015-02-25
XINJIANG NORMAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This has brought more capital investment and production difficulty to artificial cultivation

Method used

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  • Electroporation conversion method for breeding high-temperature pleuratus ferulae
  • Electroporation conversion method for breeding high-temperature pleuratus ferulae
  • Electroporation conversion method for breeding high-temperature pleuratus ferulae

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Embodiment 1

[0020] Example 1, as attached Figures 1 to 5 As shown, the method for breeding high temperature type Ferula mushroom by this electric excitation transformation method is carried out according to the following steps:

[0021] The first step, the establishment of the protoplast preparation and regeneration system of Asafoetida mushrooms: inoculate the selected Asafoetida mushrooms to further expand the culture of mushroom species; prepare protoplasts by enzymatic hydrolysis, and after obtaining relatively pure protoplasts, microscopic examination, using The best conditions for preparing protoplasts were as follows: under the condition of 0.6 mol / L KCl osmotic stabilizer, 0.1 g of 8-day-old mycelium of Asafoetida was prepared in 0.8 ml mixed enzymatic hydrolysis solution. Protoplasts, the mixed enzymatic hydrolysis solution is an aqueous solution containing 1.5% isolated enzyme, 0.5% cellulase, and 2% lysozyme, and the protoplasts were obtained by enzymatic hydrolysis at 35 ...

Embodiment 2

[0026] Embodiment 2, the difference with Example 1 is: the high temperature type Ferula mushroom strain of above-mentioned embodiment 1 gained is in the existing well-known Ferula mushroom growth environment that daytime temperature is 25 ℃, night temperature is 17 ℃. The fruiting test (same as the existing known production method of Asafoetida mushroom) was carried out, and the fruiting time was advanced by 30 days, from 120 days to 90 days. Among them, it takes about 15 days from entering the shed to the appearance of the primordium, and 5-7 days from the young mushroom to the adult mushroom.

[0027] In the present invention: unless otherwise specified, the unit of percentage % is mass percentage.

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Abstract

The invention relates to the technical field of cultivating edible fungus varieties, and relates to a method for breeding high-temperature type Ferulicum ferulae by electric shock transformation, which includes the first step of preparing the protoplasts of Ferula ferulae and the establishment of a regeneration system, and the second step of exogenous gene The third step is the introduction of exogenous genes into the protoplasts of Ferulica feruli by electric shock method, the fourth step is the selection of excellent regenerated strains, and the fifth step is the identification of transformants. Compared with the prior art, the present invention obtains a new germplasm of high-temperature type ferulae ferulae with fast growth rate and normal fruiting without low-temperature stimulation. The fruiting temperature is 25°C during the day and 17°C at night, and The mushroom time was advanced 30 days. In terms of economic benefits, the invention effectively improves the quality of Ferulicum ferulae, not only shortens the growth cycle and saves cultivation costs, but also enables off-season production, which really contributes to increasing farmers' income.

Description

technical field [0001] The invention relates to the technical field of cultivating edible fungus varieties, and relates to a method for breeding high-temperature type Ferulicum ferulae by electric shock transformation. Background technique [0002] Asafoetida ( Pleurotus ferulae ) The Chinese name is Junggar ferulae, which is a low-temperature mushroom that requires fresh air and is suitable for cultivation in humid environments in warm winter and cool summer regions. The optimum temperature for mycelium growth is 25°C to 28°C, and mycelium stops growing at 35°C to 36°C. The differentiation temperature of the fruiting bodies of Ferulicum ferulae can be grown at 0°C to 15°C, and the fruiting bodies can grow at 5°C to 18°C, and the growth is faster and the mushroom quality is better at 13°C to 15°C. Asafoetida must be stimulated by low temperature to pass physiological maturity, otherwise it is difficult to produce mushrooms. The water content of the third stage of ferulic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/15C12R1/645
Inventor 曾卫军孙艺铎祝长青曹兴芹李学斌王勇迟庆杰刘凤卫
Owner XINJIANG NORMAL UNIVERSITY
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