Plant regeneration method of humid euphoriba somatic embryogenesis path and culture medium for said method

A technology of culture medium and proliferation medium, which is applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve the problem that callus has difficulty in long-term subculture differentiation ability, low reproduction efficiency, and difficulty in realizing industrial production, etc. To achieve the effect of improving callus induction rate and somatic embryo differentiation rate and good proliferation effect

Inactive Publication Date: 2008-07-02
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to subculture and maintain the differentiation ability for long-term subculture and maintain the differentiation ability of the callus produced by the Dijin tissue or cell culture under the above-mentioned in vitro conditions, so it is difficult to be the recipient of genetic transformation
At the same time, the prior art reports that the tissue culture of broccoli has low reproductive efficiency and is difficult to realize factory production.

Method used

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  • Plant regeneration method of humid euphoriba somatic embryogenesis path and culture medium for said method
  • Plant regeneration method of humid euphoriba somatic embryogenesis path and culture medium for said method
  • Plant regeneration method of humid euphoriba somatic embryogenesis path and culture medium for said method

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Effect test

Embodiment 1

[0040] Test material selection, medium design and inoculation culture

[0041] 1. Source of test materials and their treatment:

[0042]The test materials of the present invention are selected from the buds and stems of the brocade band, collected from the flower test base of Huazhong Agricultural University, Wuhan City, Hubei Province, and each plant of the brocade is a genotype. According to the difference in differentiation rate, the applicant will collect six brocade plants and number them PTC, PT1, PT2, PT3, PT4, PT5 respectively. The stems of Radix chinensis collected in the field are sterilized according to conventional methods (refer to: Compiled by Li Mingjun, Plant Tissue Culture, China Agricultural Publishing House, 1992 edition), inoculate the stems of Radix radiata after disinfection in MS basic medium+6-BA1.0mg / L medium to induce axillary bud germination to obtain aseptic seedlings, and inoculate them on MS basic medium + 6-BA1.5mg / L + IAA0.01mg / L medium to pro...

Embodiment 2

[0060] Effects of Different Concentrations of 2,4-D on Callus Induction of Petiole of Radix Digitata

[0061] In plant tissue culture, 2,4-D is a widely used auxin growth regulator, and it is recognized as the most effective auxin for initiating cell dedifferentiation, forming callus or organogenesis .

[0062] After cultured for about 1 week, a small amount of white callus can be seen at the enlarged incision. After about 1 month, two types of callus can be observed, one is white and translucent, firmer, with Glossy with few adventitious roots (see figure 1 A), most of the callus can produce somatic embryos in subsequent subcultures. The other is white, loose and vigorous growth or water-stained or brown. The state of this callus is difficult to adjust, and somatic embryos cannot be produced during differentiation. In the method of the present invention, the petioles of the six genotypes tested can all produce callus on the medium of four different concentrations of 2,4-D,...

Embodiment 3

[0067] Effects of Different Concentrations of BA and NAA on the Induction of Somatic Embryos from Petiole Callus

[0068] Phytohormones play an important role in somatic embryo differentiation and seedling formation, and can stimulate somatic cells to form embryos by changing the level of auxin or the ratio of auxin / cytokinin. In order to explore the effects of BA and NAA on somatic embryo induction, the callus derived from genotype coded PTC (after subculture twice) was inoculated on different somatic embryo induction media (see Table 3).

[0069] Different concentrations of 6-BA and NAA and their interaction had significant effects on somatic embryo induction. LSD analysis showed that there were significant differences between BA1.5mg / L and BA1.0mg / L and BA2.0mg / L, but there was no significant difference between BA1.0mg / L and BA2.0mg / L. When the NAA concentration is within a certain range (0.01-0.05mg / L), with the increase of BA concentration, the somatic embryo differentia...

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Abstract

The present invention relates to plant tissue and cell culture technology, and is especially the plant regeneration process via humid euphorbia somatic embryogenesis path. The process includes the following steps: A. culturing humid euphorbia explant in culture medicine with inducing agent and inducing callus from the explant; B. inoculating the callus to proliferation culture medium for proliferation; C. inoculating well developed callus to somatic embryo inducing culture medium to induce somatic embryo differentiation or generate fragile embryonic callus, and inoculating the fragile embryonic callus to other culture medium for secondary culture or differentiation; D. inoculating the un-mature somatic embryo to somatic embryo proliferating culture medium for proliferation and further differentiation; and E. inoculating the mature somatic embryo to seedling culture medium to culture complete plant. The present invention also relates to the different culture mediums for the process.

Description

technical field [0001] The invention belongs to the technical field of plant tissue and cell culture, and is related to the rapid propagation of garden plants, and specifically relates to a method for regenerating a Radix Polygonum plant through somatic cell generation using petioles and stems of Radix Radix Radix as explants and the method used in the method culture medium. Background technique [0002] Parthenocissus tricuspidata Planch, also known as wall-climbing tiger, belongs to the genus Parthenocissus tricuspidata Planch. The plant grows rapidly, has strong climbing ability, thick green leaves in summer, purple leaves in autumn, wide adaptability and good sand-fixing ability. cover vegetation. [0003] In the prior art, the plant regeneration method of Dijin somatic cell generation pathway is usually such that immature embryos or cotyledon leaves and petioles are inoculated on the induction medium to induce callus, and the induced callus is subcultured, Then trans...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 包满珠阳永学刘国锋张俊卫高丽萍胡惠蓉叶要妹
Owner HUAZHONG AGRI UNIV
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