Method for regenerating Chinese rose plant by using immature seed as explant

A rose plant technology, applied in the field of rose plant regeneration through somatic embryogenesis, to achieve good multiplication effect

Inactive Publication Date: 2012-05-23
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the induction of somatic embryos in rose 'Ziwu' at home and abroad.

Method used

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  • Method for regenerating Chinese rose plant by using immature seed as explant
  • Method for regenerating Chinese rose plant by using immature seed as explant
  • Method for regenerating Chinese rose plant by using immature seed as explant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Test material selection, medium design and inoculation culture

[0029] 1. The source and treatment of test materials:

[0030] The test material among the present invention is selected from and takes to grow robustly in the flower base of Huazhong Agricultural University, the excellent rose variety "Ziwu" without disease and insect pests (the original source variety is purchased from the flower nursery stock market of Hubei Academy of Agricultural Sciences, Wuchang, Wuhan City, Hubei Province. unripe fruit (8 weeks after free pollination). Fruit processing method: cut open the collected immature fruit of the Chinese rose 'Ziwu', take out the seeds inside, and select plump seeds that can sink in clear water. After rinsing with tap water for 30 minutes, put them into the mixed solution (concentrated sulfuric acid by volume: water=3:2) to soak the seeds for 30 minutes. Disinfect with 70% alcohol for 40 seconds on the ultra-clean workbench, then disinfect with 0.1% mercu...

Embodiment 2

[0045] Effects of different concentration combinations of 2, 4-D and 6-BA on somatic embryogenesis in rose 'Ziwu'

[0046] The immature zygotic embryos of rose 'Ziwu' were used as explants to induce somatic embryogenesis. When the concentration of 2,4-D was 2mg / L, the highest induction rate of 'Ziwu' somatic embryos was 11.11%. Some immature zygotic embryos that did not generate somatic embryos were browned and died, and some produced non-embryogenic callus. The experimental results are shown in Table 2:

[0047] Table 2 Effects of different concentration combinations of 2, 4-D and 6-BA on somatic embryogenesis in rose 'Ziwu'

[0048]

Embodiment 3

[0050] Effects of Different Media on Germination of Somatic Embryos and Plantlet Regeneration of Rose 'Ziwu'

[0051] The effects of different media on the germination and plant regeneration of rose 'Ziwu' somatic embryos are shown in Table 3: In the somatic embryo germination test, there were significant differences in the effects of 9 media on the germination of rose somatic embryos . On MS+0.5mg / L 6-BA+3%Glucose+0.25%GEL medium, the germination rate of 'Ziwu' somatic embryos was the highest, reaching 86.67%, and the seedling rate per unit of somatic embryos was as high as 98.88%. 6-BA did not significantly promote the germination of somatic embryos and plant regeneration of rose 'Ziwu', and the effect of 6-BA was better than that of TDZ. During the plant regeneration process of somatic embryos of rose 'Ziwu', roots and buds occur simultaneously, and a complete plant with roots and buds can be formed without rooting culture.

[0052] Table 3 Effects of different seedling g...

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Abstract

The invention belongs to the technical field of tissue and cell culture of plants and in particular relates to a method for regenerating a plant in Chinese rose somatic embryogenesis. The method comprises the follow three cultivating stages of: (1) cultivating a Chinese rose explant on an induction culture medium and directly inducing somatic embryo from the explant, wherein the explant is an immature zygotic embryo of a Chinese rose; (2) inoculating the somatic embryo to a proliferating culture medium for proliferation and promoting the further differentiation of the somatic embryo; and (3) inoculating the somatic embryo subjected to the proliferating culture to a somatic embryo seedling culture medium so as to regenerate an integrated plant. In the Chinese rose somatic embryo subjected to isolated culture, roots and shoots can grow at the same time during the regeneration of the plant, so that the integrated plant can be formed without rooting culture. The method has the advantages of wide sources of the explant, readily available raw materials and the like. The obtained somatic embryo is a good agrobactrium rhizogenes-mediated and genetically transformed receptor. The method can also be applied to the research of the genetic transformation of Chinese roses.

Description

technical field [0001] The invention belongs to the technical field of plant tissue and cell culture, and specifically relates to a method for regenerating rose plants through somatic embryogenesis by using immature zygotic embryos of roses as explants. Background technique [0002] Rosa hybrida is a perennial evergreen woody plant belonging to the genus Rosa of the family Rosaceae. Rose has a long history of cultivation and is widely planted by countries all over the world. It is a very important ornamental flower with important commercial value and plays an important role in landscaping. Rose is known as the queen of flowers because of its graceful posture, rich and gorgeous flower colors, rich fragrance, long flowering period, strong adaptability, easy reproduction and convenient management. Roses can be used in a wide range of ways. For example, vine roses can be used to decorate corridors and arches, tree roses can be used to decorate main roads, shrub roses can be use...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 包满珠刘国锋包颖李贝张俊卫宁国贵傅小鹏
Owner HUAZHONG AGRI UNIV
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