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Tissue culture method of red-leaf sacred bamboo

A technique for tissue culture and Nandina, applied in the field of plant cultivation, can solve problems such as restricting the large-scale promotion of red-leaf Nandina, and achieve the effects of fast reproduction, high survival rate, and broad market prospects.

Active Publication Date: 2011-08-03
ZHEJIANG SENHE SEED +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic reports on tissue culture regeneration of Nandina, and there is no report on the tissue culture of Nandina, which limits the large-scale promotion of Nandina to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Select healthy plants with typical ornamental properties from the red-leaf nandina seedlings as materials, spray 800 times carbendazim on the whole plant every 8 days one month before collecting new branches, and cut them in the morning on sunny days from March to May every year. Take new branches, remove the pinnate leaves, wash the dust on the surface with tap water, then soak in detergent solution for 7 minutes, and then rinse with tap water for 0.5h for later use;

[0022] 2) On the ultra-clean workbench, soak the cleaned branches with 75% alcohol solution for 25s, pour off the alcohol, rinse with sterile water for 5 times, then add 0.1% mercury liter solution to soak for 9 minutes, shake it constantly, and then Pour off the mercuric chloride solution and rinse it with sterile water 8 times for later use;

[0023] 3) Use a sterilized blade to peel off the shoot tip on the branch, cut off the tip of the shoot tip with a length of 5mm, insert it into the prepared d...

Embodiment 2

[0027] In step 1), spray 700 times of carbendazim on the whole plant every 7 days, soak in detergent solution for 5 minutes, and rinse with tap water for 0.3 hours; in step 2), the alcohol concentration is 70%, soaking time is 30 seconds, pour off the alcohol and use sterile water Rinse 4 times, then add 0.05% mercuric chloride solution, soak for 10 min, shake continuously during this period, pour off the mercuric chloride solution and rinse with sterile water 7 times; in step 3), cut off the part with a tip length of 3mm, culture medium The formula is MS medium, add 0.5mg·L -1 BA, 0.15mg·L -1 Made with IBA, 2% sucrose and 0.8% agar until the pH of the medium is 5.8; the culture temperature in step 4) is 27°C, the light time is 11h / d, and the light intensity is 2000Lx; The shoots were cut off and divided into 0.6cm stem segments; the proliferation medium formula was MS medium with 0.5 mg·L -1 BA, 0.15 mg L -1 Made with IBA, 4% sucrose and 0.8% agar until the pH of the med...

Embodiment 3

[0028] Example 3: In step 1), the whole plant was sprayed with 900 times carbendazim every 9 days, soaked in detergent solution for 10 minutes, and rinsed with tap water for 0.6 hours; in step 2), the alcohol concentration was 80%, the soaking time was 20s, and then 0.15 % mercuric chloride solution, soaking time is 9 min, shake continuously during this period, pour off the mercuric chloride solution and rinse with sterile water for 7 times; in step 3), cut off the part with a tip length of 4mm, and the medium formula is MS medium 1.5 mg·L -1 BA, 0.05mg L -1 Made with IBA, 5% sucrose and 0.5% agar until the pH of the medium is 5.9; the culture temperature in step 4) is 26°C, the light time is 11h / d, and the light intensity is 1600Lx; The shoots were cut off and divided into 1.0cm stem segments; the proliferation medium formula was MS medium with 1.5mg L -1BA, 0.05mg L -1 Made with IBA, 5% sucrose and 0.6% agar until the pH of the medium was 5.9; in step 5), the stems wer...

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PUM

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Abstract

The invention discloses a tissue culture method of red-leaf sacred bamboo, which belongs to the technical field of plant cultivation. The method comprises the following steps of: (1) selecting a healthy plant with typical ornamental character performance to serve as a material from red-leaf sacred bamboo seedling plants, cutting new sprouts and removing pinnately compound leaves in the morning ofa sunny day, and cleaning for later use; (2) soaking, disinfecting and flushing a cleaned branch with an alcohol solution and a mercury bichloride solution in sequence on an ultra-clean workbench; (3) stripping a stem apex in the branch, cutting a tip part and inoculating into a culture medium; (4) culturing for a certain period of time and transferring a plurality of indefinite buds formed by differentiation and proliferation of the stem apex into an enrichment medium for culturing; and (5) culturing for a certain period of time, cutting immature stems growing on the indefinite buds and culturing on a rooting medium to form a robust tissue culture rooted seedling. The tissue culture method has the advantages of high survival rate, high reproduction speed, multiplication coefficient of upto 7.3 and rooting rate of up to 87 percent. The obtained red-leaf sacred bamboo has high quality and wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, in particular to a tissue culture method for red-leaf nandina. Background technique [0002] Red-leaf Nandina belongs to Berberidaceae Berberidaceae Nandina Nandina A new cultivated variety of "Zhejiang Senhe Seed Industry Co., Ltd." was selected from the Nantian bamboo cultivation group. Red-leaved nandina is an evergreen shrub with a plant height of about 80cm. It grows in clusters and has few branches. It has dense branches and leaves and a compact tree shape. The common nandina is 8-10cm in length; the leaves are compound leaves with two rounds and three leaflets, with obtuse apex, cuneate base, entire margin, thin leathery, 3-5cm long, 1.5-2cm wide, and 1.5-3cm petiole. Red-leaved Nandina likes half-shade, and can grow normally under strong light. It likes warm climate, fertile, moist and well-drained soil; the leaves that are exposed to direct sunlight in winter all turn red, an...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 郑勇平邵春荣王春张俊林
Owner ZHEJIANG SENHE SEED
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