Quick cultivation method for rhizoma bletillae tissue culture seedlings

A cultivation method and technology for tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as inability to start, lack of seedlings, and difficulty in large-scale development, saving time, high differentiation rate, short time effect

Inactive Publication Date: 2015-07-01
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Driven by profit, growers began to seek white and artificial cultivation, but due to the extreme shortage of seedlings, it was difficult to develop on a large scale, or even start

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Aseptic germination of white and seed

[0018] 1. Preparation of culture medium

[0019] Using C medium, add each mother liquor in turn, NAA 1.0mg / L, potato 200g / L (cut into pieces and boil for 0.5h, only take juice), banana 40g / L for beating, sucrose 30g / L, agar 4g / L. Constant volume, measure the pH value to about 6.0.

[0020] 2. White and capsule / seed disinfection

[0021] This operation was performed on a clean bench. If the capsules of Baiji are intact and uncracked, wipe the capsules of Baiji thoroughly with 75% alcohol, or soak for 30s, then pour out the alcohol; if the capsules are cracked, wrap the seeds in a small bag made of high-density silk stockings, and the disinfection method is the same forward.

[0022] The above-mentioned capsules / seeds were then sterilized with 0.2% mercuric chloride for 10 minutes, and fully rinsed with sterile water for 6 times. Let the capsules / seeds dry completely on a clean bench.

[0023] 3. White and seed inoculation ...

Embodiment 2

[0035] On December 18, 2013, the experiment of aseptic germination of Baiji seeds was carried out in the 6301-6303 laboratory of Yijianji Building, Yunnan University of Traditional Chinese Medicine. Select 10 capsules and sow 400 bottles in total. Because of the small size of the white and the seeds, and the inconsistent maturity among the capsules, the specific emergence rate can only be roughly estimated to be greater than 80%.

[0036] After cultivating for more than three months, on March 25, 2014, the white and seedlings that had grown to a height of 2 to 3 centimeters were transferred to the strong seedling medium for subculture in clumps of 3 to 4.

[0037] After more than three months of cultivation, on July 15, 2014, the Baiji seedlings that had grown pseudobulbs and roots were sent to the Kunming Xiaoshao planting base. After ten days of hardening, the seedlings were planted in the ground, covered with pine needles and built with shade nets. After two months, most ...

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PUM

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Abstract

The invention discloses a quick cultivation method for rhizoma bletillae tissue culture seedlings. The method comprises the following steps: selecting intact non-cracked rhizoma bletillae capsules and disinfecting the capsules by 75% alcohol for 30 seconds; then soaking and disinfecting the capsules by using 0.2% mercury bichloride for 10 minutes; washing 6 times by sterile water; fully and thoroughly washing, airing and uniformly spreading on a culture medium to cultivate; and when the seeds germinate to obtain seedlings which are about 2cm long, inoculating 3-4 seedlings as a cluster to a strong sprout culture medium to be cultured. Compared with the conventional tissue culture seedlings obtained by sterile germination of rhizoma bletillae seeds, the method just needs subculture once, so that the time consumed is short and at least three months are saved.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, and in particular relates to a rapid cultivation method for tissue-cultured seedlings of baijiu. Background technique [0002] Baiji is a perennial herb. Pseudobulb tuberous, white, plump, with finger-like divergences. The flowers are large and beautiful, purple-red, suitable for cultivation and viewing. Pseudobulb contains ingredients such as white gelatin, starch, volatile oil, etc. It has the effects of astringent, nourishing the lungs, hemostasis, and detumescence. It can be taken orally and applied externally. Ulcer bleeding embolism. Baiji is an increasingly endangered wild plant. The conditions for seed germination are harsh, and it needs to live in symbiosis with fungi. In addition, it has a long seedling period and is sensitive to the environment. Therefore, it is difficult to effectively reproduce through seeds under natural environmental conditions. Wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李宏哲蔡忠虎熊浩宇张超彭安顺杨林芳夏光惠姚雪娇岳蕾
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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